A micromachined integratable thermal reactor

Abstract

This paper presents a micromachined thermal reactor. Silicon substrate remains unheated because of thermal isolation design, during thermal cycling of the reaction chamber. Employed side-heating concept has significantly improved in-chamber temperature uniformity. Finite-element-analysis is carried out to optimise the thermal performance. Experimental results have proved that the thermal reactor can easily be integrated with other non-thermal components. Integration of the device with other components or modules of miniaturised total analysis systems (pTAS) is very promising .

PCR thermal management in an integrated Lab on Chip

“…In symmetrical conditions, the model can be considered as one dimensional and thermal resistances on both sides of the PCR chamber can be replaced by equalent thermal resistance R th . Temperature distribution of this simplified one dimensional PCR system can be given by [4] x l…”

“…Polymerase chain reaction (PCR) chamber technology for DNA amplification is well established and has been around for almost two decades. Over the years many groups have developed miniaturized PCR chips using MEMS technology [1][2][3][4], temperature uniformity has been improved tremendously and miniaturization has played a key role in this advancement. In our previous work [3][4], we had reported silicon and plastic MEMS PCR technologies with very good DNA amplification results.…”