A microfabricated fluorescence-activated cell sorter

Fu, Anne Y.; Spence, Charles; Scherer, Axel; Arnold, Frances H.; Quake, Stephen R.

doi:10.1038/15095

Cited by 844 publications

(602 citation statements)

References 13 publications

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“…2h. These monodisperse conjugated polymer particles, both spherical or anisometric, could be excellently suited for fluorescent tagging or as tracers in biological experiments, such as flow cytometry 31 or in vivo microrheology 32 .…”

Section: Resultsmentioning

confidence: 99%

Monodisperse conjugated polymer particles by Suzuki–Miyaura dispersion polymerization

2012

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The self-assembly of colloidal building blocks into complex and hierarchical structures offers a versatile and powerful toolbox for the creation of new photonic and optoelectronic materials. However, well-defined and monodisperse colloids of semiconducting polymers, which would form excellent building blocks for such self-assembled materials, are not readily available. Here we report the first demonstration of a suzuki-miyaura dispersion polymerization; this method produces highly monodisperse submicrometer particles of a variety of semiconducting polymers. moreover, we show that these monodisperse particles readily self-assemble into photonic crystals that exhibit a pronounced photonic stopgap.

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Section: Resultsmentioning

confidence: 99%

Monodisperse conjugated polymer particles by Suzuki–Miyaura dispersion polymerization

2012

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“…Referred to as micro-Total Analysis Systems (m-TAS) or Lab-on-a-Chip (LOC), these devices perform techniques such as cell culturing, Fluorescence Activated Cell Sorting (FACS), 10 and Polymerase Chain Reaction (PCR) 11 on one small and disposable chip.…”

Section: Professor Luke P Lee Is L L O Y D D I S T I N G U I S H E Dmentioning

confidence: 99%

Microfluidics-based systems biology

2006

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Systems biology seeks to develop a complete understanding of cellular mechanisms by studying the functions of intra-and inter-cellular molecular interactions that trigger and coordinate cellular events. However, the complexity of biological systems causes accurate and precise systems biology experimentation to be a difficult task. Most biological experimentation focuses on highly detailed investigation of a single signaling mechanism, which lacks the throughput necessary to reconstruct the entirety of the biological system, while high-throughput testing often lacks the fidelity and detail necessary to fully comprehend the mechanisms of signal propagation. Systems biology experimentation, however, can benefit greatly from the progress in the development of microfluidic devices. Microfluidics provides the opportunity to study cells effectively on both a single-and multi-cellular level with high-resolution and localized application of experimental conditions with biomimetic physiological conditions. Additionally, the ability to massively array devices on a chip opens the door for high-throughput, high fidelity experimentation to aid in accurate and precise unraveling of the intertwined signaling systems that compose the inner workings of the cell.

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“…Third, reduction of the distance from the sample line to injection is imperative due to the cubic relation of this distance to particle dead time. Construction of micro fluidic flow cells has been achieved (20,21) and specialized versions of these cells with precisely designed micro fluidic sample lines and small S 0 distances may find their ideal application within rapid delivery for flow cytometry. Finally, the volume of the cytometer access valve contributes to the dead volume and pressure limitations imposed by the valves limit the use of sheath control and, therefore, small volume high-pressure valves are desirable.…”

Section: Proposed Improvements To the Systemmentioning

confidence: 99%

Nozzle design parameters and their effects on rapid sample delivery in flow cytometry

et al. 2002

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Background: Rapid kinetic and high throughput flow cytometry are emerging as valuable tools in biotechnology research applications ranging from mechanistic analysis of molecular assemblies to high throughput screening. Many of these new applications have been made possible by improved sample delivery capabilities, focusing increased attention on fluidic issues associated with rapid sample delivery. Methods: Using basic fluidic premises, we derived a model that predicted the effect of nozzle parameters during rapid sample delivery. We tested the model using the rapid mix flow cytometer and modifications were made to the equipment to optimize performance. Results: The model predicted that shorter nozzles with wide exit orifices decrease the delay before initial particle analysis and the fluidic stabilization time. Experimental

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A microfabricated fluorescence-activated cell sorter

Cited by 844 publications

References 13 publications

Monodisperse conjugated polymer particles by Suzuki–Miyaura dispersion polymerization

Monodisperse conjugated polymer particles by Suzuki–Miyaura dispersion polymerization

Microfluidics-based systems biology

Nozzle design parameters and their effects on rapid sample delivery in flow cytometry

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