A method of isolating and analysing drugs from cancer cells for preclinical research

Placha, Wojciech; Zagajewski, Jacek; Suder, Piotr; Piwowar, M.

doi:10.1016/j.chroma.2022.463500

Cited by 1 publication

(2 citation statements)

References 39 publications

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“…Caution in making this hypothesis was dictated by the effect of atorvastatin. It was verified [31] by analyzing the absorbed drug into melanoma cells using HPLC, confirmed by mass analysis, that pravastatin was the only one tested that did not increase the absorption of docetaxel into melanoma cells. In the case of the use of atorvastatin with docetaxel, an increased amount of docetaxel was observed in melanoma cells in all cell types derived from different stages of cancer progression.…”

Section: Discussionmentioning

confidence: 84%

“…The cells were counted using a Countess Invitrogen TM counter. The preparation of samples for RP-HPLC analysis and the applied analysis using the RP-HPLC method are described in [31]. Mass spectrometry analysis of the selected active compounds was carried out (description in the Supplementary Materials).…”

Section: Analysis Of the Absorption Of Pharmaceuticals Into Human Mel...mentioning

confidence: 99%

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The Blocking of Drug Resistance Channels by Selected Hydrophobic Statins in Chemoresistance Human Melanoma

Placha,

Suder,

Panek

et al. 2023

Biomolecules

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Despite the development of modern drugs, drug resistance in oncology remains the main factor limiting the curability of patients. This paper shows the use of a group of hydrophobic statins to inhibit drug resistance (Pgp protein). In a chemoresistance melanoma cell model, viability, necroptosis with DNA damage, the absorption of the applied pharmaceuticals, and the functional activity of the ABCB1 drug transporter after administration of docetaxel or docetaxel with a selected hydrophobic statin were studied. Taxol-resistant human melanoma cells from three stages of development were used as a model: both A375P and WM239A metastatic lines and radial growth phase WM35 cells. An animal model (Mus musculus SCID) was developed for the A375P cell line. The results show that hydrophobic statins administered with docetaxel increase the accumulation of the drug in the tumor cell a.o. by blocking the ABCB1 channel. They reduce taxol-induced drug resistance. The tumor size reduction was observed after the drug combination was administrated. It was shown that the structural similarity of statins is of secondary importance, e.g., pravastatin and simvastatin. Using cytostatics in the presence of hydrophobic statins increases their effectiveness while reducing their overall toxicity.

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Section: Discussionmentioning

confidence: 84%

Section: Analysis Of the Absorption Of Pharmaceuticals Into Human Mel...mentioning

confidence: 99%

The Blocking of Drug Resistance Channels by Selected Hydrophobic Statins in Chemoresistance Human Melanoma

Placha,

Suder,

Panek

et al. 2023

Biomolecules

View full text Add to dashboard Cite

show abstract

A method of isolating and analysing drugs from cancer cells for preclinical research

Cited by 1 publication

References 39 publications

The Blocking of Drug Resistance Channels by Selected Hydrophobic Statins in Chemoresistance Human Melanoma

The Blocking of Drug Resistance Channels by Selected Hydrophobic Statins in Chemoresistance Human Melanoma

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