A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

Polacchini, Alessio; Metelli, Giuliana; Francavilla, Ruggiero; Baj, Gabriele; Florean, M; Mascaretti, L; Tongiorgi, Enrico

doi:10.1038/srep17989

Cited by 190 publications

(181 citation statements)

References 60 publications

(99 reference statements)

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“…Similarly, the ELISA kits used to measure BDNF have different performance in terms of detection range, sensitivity, and inter-assay variations [62]. In the studies included in our meta-analysis, all the authors correctly specified the producers; however, it cannot be excluded that inter-kit variability plays an important role in BDNF measurements.…”

Section: Discussionmentioning

confidence: 99%

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Sandrini

Minno

Amadio

et al. 2018

IJMS

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Reduction in brain-derived neurotrophic factor (BDNF) expression in the brain as well as mutations in BDNF gene and/or of its receptor are associated to obesity in both human and animal models. However, the association between circulating levels of BDNF and obesity is still not defined. To answer this question, we performed a meta-analysis carrying out a systematic search in electronic databases. Ten studies (307 obese patients and 236 controls) were included in the analysis. Our data show that obese patients have levels of BDNF similar to those of controls (SMD: 0.01, 95% CI: −0.28, 0.30, p = 0.94). The lack of difference was further confirmed both in studies in which BDNF levels were assessed in serum (MD: −0.93 ng/mL, 95% CI: −3.34, 1.48, p = 0.45) and in plasma (MD: 0.15 ng/mL, 95% CI: −0.09, 0.39, p = 0.23). Data evaluation has shown that some bias might affect BDNF measurements (e.g., subject recruitment, procedures of sampling, handling, and storage), leading to a difficult interpretation of the results. Standardization of the procedures is still needed to reach strong, affordable, and reliable conclusions.

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Section: Discussionmentioning

confidence: 99%

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Sandrini

Minno

Amadio

et al. 2018

IJMS

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“…The only published reports that we could find with such claims presented no supporting data (Chen et al, 2006; Yoshida et al, 2012). Further, a recent report concerning claims of selectivity used dot blotting, instead of an ELISA approach, for the evaluation (Polacchini et al, 2015). For these reasons, we tested the ability of the BDNF Emax ImmunoAssay kit (Promega) to detect proBDNF and mature BDNF using purified human proBDNF and mature BDNF, and, under identical assay conditions, we found that only mature BDNF was being detected.…”

Section: Discussionmentioning

confidence: 99%

Activation of the sigma-1 receptor by haloperidol metabolites facilitates brain-derived neurotrophic factor secretion from human astroglia

Dalwadi

Kim

Schetz

2017

Neurochemistry International

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Glial cells play a critical role in neuronal support which includes the production and release of the neurotrophin brain-derived neurotrophic factor (BDNF). Activation of the sigma-1 receptor (S1R) has been shown to attenuate inflammatory stress-mediated brain injuries, and there is emerging evidence that this may involve a BDNF-dependent mechanism. In this report we studied S1R-mediated BDNF release from human astrocytic glial cells. Astrocytes express the S1R, which mediates BDNF release when stimulated with the prototypical S1R agonists 4-PPBP and (+)-SKF10047. This effect could be antagonized by a selective concentration of the S1R antagonist BD1063. Haloperidol is known to have high affinity interactions with the S1R, yet it was unable to facilitate BDNF release. Remarkably, however, two metabolites of haloperidol, haloperidol I and haloperidol II (reduced haloperidol), were discovered to facilitate BDNF secretion and this effect was antagonized by BD1063. Neither 4-PPBP, nor either of the haloperidol metabolites affected the level of BDNF mRNA as assessed by qPCR. These results demonstrate for the first time that haloperidol metabolites I and II facilitate the secretion of BDNF from astrocytes by acting as functionally selective S1R agonists.

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“…The manufacturer of the ELISA kit we use claims 13% cross-reactivity for recombinant human pro-BDNF. The Elisa kit we used has a very low cross-reactivity for proBDNF compared to the other available ELISA kits (see Polacchini et al [55] and Lim et al [56]),…”

Section: Methodsmentioning

confidence: 99%

BDNF Responses in Healthy Older Persons to 35 Minutes of Physical Exercise, Cognitive Training, and Mindfulness: Associations with Working Memory Function

Håkansson

Ledreux

Daffner

et al. 2016

JAD

155

117

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Brain-derived neurotrophic factor (BDNF) has a central role in brain plasticity by mediating changes in cortical thickness and synaptic density in response to physical activity and environmental enrichment. Previous studies suggest that physical exercise can augment BDNF levels, both in serum and the brain, but no other study has examined how different types of activities compare with physical exercise in their ability to affect BDNF levels. By using a balanced cross over experimental design, we exposed nineteen healthy older adults to 35-minute sessions of physical exercise, cognitive training, and mindfulness practice, and compared the resulting changes in mature BDNF levels between the three activities. We show that a single bout of physical exercise has significantly larger impact on serum BDNF levels than either cognitive training or mindfulness practice in the same persons. This is the first study on immediate BDNF effects of physical activity in older healthy humans and also the first study to demonstrate an association between serum BDNF responsivity to acute physical exercise and working memory function. We conclude that the BDNF increase we found after physical exercise more probably has a peripheral than a central origin, but that the association between post-intervention BDNF levels and cognitive function could have implications for BDNF responsivity in serum as a potential marker of cognitive health.

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A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

Cited by 190 publications

References 60 publications

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Association between Obesity and Circulating Brain-Derived Neurotrophic Factor (BDNF) Levels: Systematic Review of Literature and Meta-Analysis

Activation of the sigma-1 receptor by haloperidol metabolites facilitates brain-derived neurotrophic factor secretion from human astroglia

BDNF Responses in Healthy Older Persons to 35 Minutes of Physical Exercise, Cognitive Training, and Mindfulness: Associations with Working Memory Function

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