A method for long-term quantitative and fractional urine collection

Liu, Hsi-Yen; Anderson, Gary J.

doi:10.1016/s0022-3476(67)80424-4

Cited by 19 publications

(8 citation statements)

References 5 publications

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“…Urine collection was made by a small purpose-made bag attached via suction pump to a collecting bottle on ice [12]. Urine volume was measured 4-hourly and di vided into aliquots for estimation of AVP and osmo lality.…”

Section: Patients Materials and Methodsmentioning

confidence: 99%

Continuous Urine Collection in the Study of Vasopressin in the Newborn

1983

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We have evaluated the use of urinary vasopressin (AVP) measurements in human newborns to see if urinary AVP excretion is a true reflection of the plasma level. AVP was measured in 37 simultaneously collected plasma and urine samples. The correlation coefficient was 0.8 (p < 0.001). AVP extracted in duplicate from six urine samples was measured by bioassay and radioimmunoassay. The correlation coefficient was 0.85 (p < 0.05). To facilitate long-term urine collection we have developed a urine collecting tray to fit a standard incubator or cot. Urinary AVP was measured in 24-hour urine collections in 37 normal infants. AVP excretion was higher in the first 48 h of life than subsequently. Continuous 4-hour urine collections were made in 1 sick infant to assess AVP excretion when urine flow is variable.

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Section: Patients Materials and Methodsmentioning

confidence: 99%

Continuous Urine Collection in the Study of Vasopressin in the Newborn

1983

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“…Urine was collected by continuous aspiration (27) into a chilled weighed flask which was changed every 4 hr. A borate buffer trap was incorporated into the circuit to collect any ammonia resulting from bacterial decomposition.…”

Section: Urine Collectionmentioning

confidence: 99%

Nitrogen Metabolism in Preterm Infants Fed Human Donor Breast Milk: the Possible Essentiality of Glycine

et al. 1981

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SummaryNitrogen metabolism was studied in three preterm infants (mean gestation 32 wk) by the method of consecutive metabolic balance. The absorption and retention of nitrogen from breast milk was measured, and protein turnover, synthesis, and breakdown were calculated from isotopic plateau of urinary urea and ammonia using an intermittent oral administration of "N-glycine. Weight gain and nitrogen retention were compared with the weight gain and nitrogen accumulated for a foetus of equivalent gestational age in utero.The average composition of the milk was 289 + 19 K J dl-' and 1.44 + 24 g protein dl-'. The intake of energy was 572 + 61 KJ kg-' day -' and of nitrogen 447 + 99 mg kg-' day-'. Stool output of nitrogen was 100 + 32 mg kg-' day-' giving an absorption of 348 + 78 mg kg-' day-', as urinary excretion was 91 + 17 mg kg-' day-' retained nitrogen was 256 2 71 mg kg-' day-', or 56% of intake.The specific weight gain was 15.6 5 2.6 g kg-' day-' and 53% of this comprised lean tissue (range 34 to 89%). In all but one study the postnatal retention of nitrogen fell far short of calculated in utero accumulation.The results of protein turnover were surprising. In six of the eight studies urinary urea failed to become enriched at all. Protein turnover calculated from the ammonia plateau was 1.94

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“…The shaw (17). urine was continuously aspirated into a chilled glass flask using the method of Liu and Anderson (9). Only the three girls were restrained on a mattress.…”

Section: Accumulation Of Magnesium Zinc and Copper By Human Fetus Bmentioning

confidence: 99%