A method for evaluating drug penetration and absorption through isolated buccal mucosa with highly accuracy and reproducibility

Oral Mucosa Models to Evaluate Drug Permeability

“…To solve this problem, in a recent paper [ 33 ], the authors utilized an MTT assay to evaluate the mucosa viability before and after permeability tests, thus comparing the cell viability of five different mucosal models extracted and preserved under different modalities and conditions. In particular, the media utilized were phosphate buffer solution (PBS), Kreb’s bicarbonate Ringer’s solution (KRP), KRP+ 1% fetal bovine serum (FBS), and KRP+ 1% FBS in a CO 2 atmosphere.…”

“…A solution of MTT was added to each well, and after 4 h of incubation, the cells were lysed, and the formazan crystals were solubilized by DMSO. Thus, the absorbance at 540 nm was measured in each well, and the viability was determined relative to fresh mucosa, which was assumed to be 100% viable [ 33 ].…”

“…Moreover, the authors reported that in the presence of selected cryoprotectants (20% glycerol and 20% trehalose), the mucosae, which were frozen at −80 °C and thawed at 37 °C, exhibited preserved integrity and biological viability for 21 days. Therefore, this study aimed to identify the experimental conditions to standardize the process of isolating, maintaining, and determining the viability of mucosa and thus improve the accuracy of permeability studies [ 33 ].…”