A Mechanosensitive GPCR that Detects the Bloody Force

Iliff, Adam J.; Xu, X.Z. Shawn

doi:10.1016/j.cell.2018.04.001

Cited by 8 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More and more mechanosensitive ion channels have been identified in mammalian cells, such as TMEM family proteins, piezo channels, transient receptor potential (TRP) superfamily, and GPCR. These channels can sense different mechanical stimuli, such as osmotic pressure, shear force, and ECM stiffness. − These studies demonstrate that mechanical cues can alter the conformation of ion channels in cell membranes, result in the flow of ions into cytoplasm, regulate the reconstruction of filamentous actin network, and ultimately impact the fate of stem cells.…”

Section: Role Of Mechanical Factors In Cell Differentiationmentioning

confidence: 98%

Mechanical Roles of F-Actin in the Differentiation of Stem Cells: A Review

Fan

Zhao

et al. 2019

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

In the development and differentiation of stem cells, mechanical forces associated with filamentous actin (F-actin) play a crucial role. The present review aims to reveal the relationship among the chemical components, microscopic structures, mechanical properties, and biological functions of F-actin. Particular attention is given to the functions of the cytoplasmic and nuclear microfilament cytoskeleton and their regulation mechanisms in the differentiation of stem cells. The distributions of different types of actin monomers in mammal cells and the functions of actin-binding proteins are summarized. We discuss how the fate of stem cells is regulated by intra/extracellular mechanical and chemical cues associated with microfilament-related proteins, intercellular adhesion molecules, etc. In addition, we also address the differentiationinduced variation in the stiffness of stem cells and the correlation between the fate and geometric shape change of stem cells. This review not only deepens our understanding of the biophysical mechanisms underlying the fates of stem cells under different culture conditions but also provides inspirations for the tissue engineering of stem cells.

show abstract

Section: Role Of Mechanical Factors In Cell Differentiationmentioning

confidence: 98%

Mechanical Roles of F-Actin in the Differentiation of Stem Cells: A Review

Fan

Zhao

et al. 2019

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…Experimental evidence strongly supports the critical role of mechanical forces in the direct activation of these receptors. Mechanical stimuli can activate GPCRs without the involvement of their cognate agonists [ 35 , 36 ]. Supporting these observations, stimuli such as shear stress, hypotonic conditions and cell stretching, that alter membrane organization, have been reported as inducers of conformational transitions of GPCRs between an inactive to an activated state [ 35 , 37 , 38 ].…”

Section: Gpcrs Regulation and Functions Beyond The Classical Modulationmentioning

confidence: 99%

“…Mechanical stimuli can activate GPCRs without the involvement of their cognate agonists [ 35 , 36 ]. Supporting these observations, stimuli such as shear stress, hypotonic conditions and cell stretching, that alter membrane organization, have been reported as inducers of conformational transitions of GPCRs between an inactive to an activated state [ 35 , 37 , 38 ]. However, there are many aspects to be explored to further clarify how GPCRs might themselves be mechano-sensors and the mechanisms and functions behind this novel regulatory pathway.…”

Section: Gpcrs Regulation and Functions Beyond The Classical Modulationmentioning

confidence: 99%

“…Recent studies have shown that extracellular N-terminus within adhesive GPCRs can act as an anchor mechanism to the extracellular matrix (ECM), playing key roles in response to mechanical tension and in the control of their activity [ 37 , 38 ]. A recent revision approaches the different GPCRs which can be directly modulated by mechanical forces, highlighting the critical role of specific GPCRs in mechanotransduction such as adhesion GPCRs, APJ/apelin, AT 1 R, B 2 AR, B 2 R, ET 1 AR, GPR68, H 1 R, M 5 R PTH 1 R, all of them very relevant at vascular level [ 35 , 36 , 39 , 40 , 41 ]. Since most GPCRs contain at least one N-glycan chain in their extracellular domain, further investigation will be required to address their patho-physiological functions.…”

Section: Gpcrs Regulation and Functions Beyond The Classical Modulationmentioning

confidence: 99%

“…Supporting this idea, it has been demonstrated that mechanical perturbations can modulate GPCR conformational transitions [ 36 ]. Moreover, the response to shear stress can be directly modulated through the Gαq-coupled GPCR, GPR68 [ 35 , 41 ]. Interestingly, PKCζ, a protein that we have described as new interactor of Gαq [ 147 ], can be regulated by shear stress and activated by disturbed flow in atheroprone areas [ 148 ].…”

Section: Autophagy Between Nutrient Mechanical and Oxidative Stress: ...mentioning

confidence: 99%

See 2 more Smart Citations

Gq Signaling in Autophagy Control: Between Chemical and Mechanical Cues

et al. 2022

View full text Add to dashboard Cite

All processes in human physiology relies on homeostatic mechanisms which require the activation of specific control circuits to adapt the changes imposed by external stimuli. One of the critical modulators of homeostatic balance is autophagy, a catabolic process that is responsible of the destruction of long-lived proteins and organelles through a lysosome degradative pathway. Identification of the mechanism underlying autophagic flux is considered of great importance as both protective and detrimental functions are linked with deregulated autophagy. At the mechanistic and regulatory levels, autophagy is activated in response to diverse stress conditions (food deprivation, hyperthermia and hypoxia), even a novel perspective highlight the potential role of physical forces in autophagy modulation. To understand the crosstalk between all these controlling mechanisms could give us new clues about the specific contribution of autophagy in a wide range of diseases including vascular disorders, inflammation and cancer. Of note, any homeostatic control critically depends in at least two additional and poorly studied interdependent components: a receptor and its downstream effectors. Addressing the selective receptors involved in autophagy regulation is an open question and represents a new area of research in this field. G-protein coupled receptors (GPCRs) represent one of the largest and druggable targets membrane receptor protein superfamily. By exerting their action through G proteins, GPCRs play fundamental roles in the control of cellular homeostasis. Novel studies have shown Gαq, a subunit of heterotrimeric G proteins, as a core modulator of mTORC1 and autophagy, suggesting a fundamental contribution of Gαq-coupled GPCRs mechanisms in the control of this homeostatic feedback loop. To address how GPCR-G proteins machinery integrates the response to different stresses including oxidative conditions and mechanical stimuli, could provide deeper insight into new signaling pathways and open potential and novel therapeutic strategies in the modulation of different pathological conditions.

show abstract

Mechanical Force Remodeling the Adult Brain

Zhang

Liu

et al. 2023

Neurosci. Bull.

View full text Add to dashboard Cite

A Mechanosensitive GPCR that Detects the Bloody Force

Cited by 8 publications

References 8 publications

Mechanical Roles of F-Actin in the Differentiation of Stem Cells: A Review

Mechanical Roles of F-Actin in the Differentiation of Stem Cells: A Review

Gq Signaling in Autophagy Control: Between Chemical and Mechanical Cues

Mechanical Force Remodeling the Adult Brain

Contact Info

Product

Resources

About