A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies

Tin, Christine; Yuan, Lijuan; Dexter, Rachel J.; Parra, Gabriel I.; Bui, Tammy; Green, Kim Y.; Sosnovtsev, Stanislav V.

doi:10.1016/j.jviromet.2017.06.017

Cited by 11 publications

(16 citation statements)

References 33 publications

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“…The assay showed the same sensitivity as the one observed when human sera were tested in parallel by the commercial ELISA (Supplemental Table 3). In addition, LIPS sensitivity has been extensively described in different studies and demonstrated to be at least as sensitive as plaque reduction neutralization test and ELISA (Burbelo et al, 2012; Tin et al, 2017; Crim et al, 2019). We therefore conclude that the different viruses we studied are not (or very rarely) transmitted to humans and cattle (<0.017% and <0.047%, respectively).…”

Section: Discussionmentioning

confidence: 99%

Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand

et al. 2019

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Emerging zoonoses caused by previously unknown agents are one of the most important challenges for human health because of their inherent inability to be predictable, conversely to emergences caused by previously known agents that could be targeted by routine surveillance programs. Emerging zoonotic infections either originate from increasing contacts between wildlife and human populations, or from the geographical expansion of hematophagous arthropods that act as vectors, this latter being more capable to impact large-scale human populations. While characterizing the viral communities from candidate vectors in high-risk geographical areas is a necessary initial step, the need to identify which viruses are able to spill over and those restricted to their hosts has recently emerged. We hypothesized that currently unknown tick-borne arboviruses could silently circulate in specific biotopes where mammals are highly exposed to tick bites, and implemented a strategy that combined high-throughput sequencing with broad-range serological techniques to both identify novel arboviruses and tick-specific viruses in a ticks/mammals interface in Thailand. The virome of Thai ticks belonging to the Rhipicephalus, Amblyomma, Dermacentor, Hyalomma, and Haemaphysalis genera identified numerous viruses, among which several viruses could be candidates for future emergence as regards to their phylogenetic relatedness with known tick-borne arboviruses. Luciferase immunoprecipitation system targeting external viral proteins of viruses identified among the Orthomyxoviridae, Phenuiviridae, Flaviviridae, Rhabdoviridae, and Chuviridae families was used to screen human and cattle Thai populations highly exposed to tick bites. Although no positive serum was detected for any of the six viruses selected, suggesting that these viruses are not infecting these vertebrates, or at very low prevalence (upper estimate 0.017% and 0.047% in humans and cattle, respectively), the virome of Thai ticks presents an extremely rich viral diversity, among which novel tick-borne arboviruses are probably hidden and could pose a public health concern if they emerge. The strategy developed in this pilot study, starting from the inventory of viral communities of hematophagous arthropods to end by the identification of viruses able (or likely unable) to infect vertebrates, is the first step in the prediction of putative new emergences and could easily be transposed to other reservoirs/vectors/susceptible hosts interfaces.

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Section: Discussionmentioning

confidence: 99%

Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand

et al. 2019

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“…As a sensory component of hybrids, various biospecific polypeptides were applied—antibodies or their fragments [ 111 , 112 , 113 , 114 ], antigens [ 115 , 116 , 117 , 118 ], polypeptides with special binding ability [ 119 , 120 , 121 , 122 ], GFP and its color variants [ 123 , 124 , 125 , 126 , 127 , 128 , 129 ], etc. Based on the phenomenon of assembling split luciferase derivatives, a whole spectrum of homogeneous methods has been developed [ 125 , 126 , 127 , 128 , 129 ] and applied for studying protein–protein/ligand interaction [ 130 , 131 , 132 , 133 ], protein activity [ 134 , 135 ], and protein folding [ 136 , 137 ].…”

Section: Ctz-dependent Luciferase Analytical Applicationmentioning

confidence: 99%

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Krasitskaya

Bashmakova

Frank

2020

IJMS

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: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

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“…antibodies) against potentially pathogenic bacteria in the HGM transplants, all piglets received 60 ml gamma-irradiated, non-heat treated porcine serum (Rocky Mountain Biologicals) via three intraperitoneal injections at 24, 30 and 36 h post derivation. The porcine serum was screened using a luciferase immunoprecipitation system assay [53], and no antibodies against a broad range of genotype of HuNoVs, including GI. [45].…”

Section: Gnotobiotic Pigs and Treatmentsmentioning

confidence: 99%

Enhanced GII.4 human norovirus infection in gnotobiotic pigs transplanted with a human gut microbiota

Lei

Twitchell

Ramesh

et al. 2019

Journal of General Virology

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The role of commensal microbiota in enteric viral infections has been explored extensively, but the interaction between human gut microbiota (HGM) and human norovirus (HuNoV) is poorly understood. In this study, we established an HGM-Transplanted gnotobiotic (Gn) pig model of HuNoV infection and disease, using an infant stool as HGM transplant and a HuNoV GII.4/2006b strain for virus inoculation. Compared to germ-free Gn pigs, HuNoV inoculation in HGMT Gn pigs resulted in increased HuNoV shedding, characterized by significantly higher shedding titres on post inoculation day (PID) 3, 4, 6, 8 and 9, and significantly longer mean duration of virus shedding. In addition, virus titres were significantly higher in duodenum and distal ileum of HGMT Gn pigs on PID10, while comparable and transient HuNoV viremia was detected in both groups. 16S rRNA gene sequencing demonstrated that HuNoV infection dramatically altered intestinal microbiota in HGMT Gn pigs at the phylum (Proteobacteria, Firmicutes and Bacteroidetes) and genus (Enterococcus, Bifidobacterium, Clostridium, Ruminococcus, Anaerococcus, Bacteroides and Lactobacillus) levels. In summary, enhanced GII.4 HuNoV infection was observed in the presence of HGM, and host microbiota was susceptible to disruption upon HuNoV infection.

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A Luciferase Immunoprecipitation System (LIPS) assay for profiling human norovirus antibodies

Cited by 11 publications

References 33 publications

Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand

Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

Enhanced GII.4 human norovirus infection in gnotobiotic pigs transplanted with a human gut microbiota

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