PLoS ONE volume 9, issue 6, Pe98431 2014 DOI: 10.1371/journal.pone.0098431 View full text
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Philippa M. Beard, Samantha J. Griffiths, Orland Gonzalez, Ismar R. Haga, Tali Pechenick Jowers, Danielle K. Reynolds, Jan Wildenhain, Hille Tekotte, Manfred Auer, Mike Tyers, Peter Ghazal, Ralf Zimmer, Jürgen Haas

Abstract: Vaccinia virus (VACV) is a large, cytoplasmic, double-stranded DNA virus that requires complex interactions with host proteins in order to replicate. To explore these interactions a functional high throughput small interfering RNA (siRNA) screen targeting 6719 druggable cellular genes was undertaken to identify host factors (HF) influencing the replication and spread of an eGFP-tagged VACV. The experimental design incorporated a low multiplicity of infection, thereby enhancing detection of cellular proteins i…

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