A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

Carvalho, Karine Maria Martins Bezerra; Nava, R.A.; França, Marcelo Santucci; Medeiros, Marco Alberto; Camarão, G.C.; Juliano, Luiz

doi:10.1590/s0100-879x1999000100007

Cited by 2 publications

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References 17 publications

(16 reference statements)

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“…6,22 In the gastrointestinal tract, CD10 expression is limited to microvilli of the small intestinal and biliary epithelium, where it regulates the concentration of bradykinin, cholecystokinin, and other bioactive substances in the postsecretory modification of bile. 4,5,24 The immunostaining pattern of CD10 in the intestinal villi, gallbladder, and bile ducts is consistently strong with continuous apical expression on the brush border, 14,21,30 whereas in hepatocytes CD10 expression is discontinuous, with patchy apical labeling with a canalicular distribution. 4,6,13,21 A few reports also showed that CD10 expression is mostly preserved in benign lesions such as hepatic bile duct adenoma, 36 pyloric gland adenoma of the gallbladder, 24 and benign intrahepatic bile ducts.…”

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confidence: 99%

Diagnostic Utility of CD10 in Benign and Malignant Extrahepatic Bile Duct Lesions

Tretiakova

Antic

Westerhoff

et al. 2012

American Journal of Surgical Pathology

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CD10, a cell surface enzyme with neutral metalloendopeptidase activity, is a marker for intestinal epithelial brush border. It is also present in normal bile ducts and gallbladder epithelia but is absent in cholangiocarcinomas. However, the expression profile of CD10 in benign and malignant extrahepatic biliary lesions has not been studied. In this study, 69 biopsies, 9 resections, and 9 cell blocks prepared from fine-needle aspirations of the extrahepatic bile ducts from 86 patients were studied immunohistochemically for CD10 expression. The majority of cases contained normal biliary epithelium (NL, n=64), along with foci of benign or malignant lesions in various combinations. Benign lesions included reactive atypia (n=35), low-grade dysplasia of unknown significance (n=21), and bile duct adenoma (BDA, n=1). Malignant lesions included high-grade dysplasia (HGD, n=45) and invasive adenocarcinoma (IC, n=30). As expected, the NL showed strong continuous staining at the apical surface in all cases. Benign lesions were also CD10 positive in all but 3 cases; however, the staining pattern was discontinuous, with positive cells varying from 20% to 80%. None of the malignant lesions showed CD10 immunoreactivity, except for 2 HGD cases and 1 IC case, which exhibited focal staining. The Pearson χ2 and Fisher exact tests showed significant statistical difference in CD10 expression among the study groups (P<0.001). Our findings suggest that absence of CD10 expression in strips of atypical biliary epithelial cells may be a phenotype associated with malignant transformation and may serve as a useful marker to aid in the evaluation of bile duct biopsies, in which distinction between benign and malignant lesions on biopsies or cytology specimens can be extremely challenging because of limited sampling, crush artifact, and frequent inflammatory/reactive changes.

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“…A metallopeptidase from human liver which degrades bradykinin and atrial natriuretic peptide was purified to homogeneity by Carvalho et al (32). Another possibility is the interference of the Thimet oligopeptidase (EC 3.4.24.15), which has been reported to be the major liver kininase in the rat (33).…”

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confidence: 99%

A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity

Alves

Araujo

Juliano

et al. 2005

Braz J Med Biol Res

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A continuous assay using internally quenched fluorescent peptides with the general sequence Abz-peptidyl-(Dnp)P-OH (Abz = ortho-aminobenzoic acid; Dnp = 2,4-dinitrophenyl) was optimized for the measurement of angiotensin I-converting enzyme (ACE) in human plasma and rat tissues. Abz-FRK(Dnp)P-OH, which was cleaved at the Arg-Lys bond by ACE, was used for the enzyme evaluation in human plasma. Enzymatic activity was monitored by continuous recording of the fluorescence (lambda ex = 320 nm and lambda em = 420 nm) at 37 degrees C, in 0.1 M Tris-HCl buffer, pH 7.0, with 50 mM NaCl and 10 microM ZnCl2. The assays can be performed directly in the cuvette of the fluorimeter and the hydrolysis followed for 5 to 10 min. ACE measurements in the plasma of 80 healthy patients with Hip-His-Leu and with Abz-FRK(Dnp)P-OH correlated closely (r = 0.90, P < 0.001). The specificity of the assay was demonstrated by the complete inhibition of hydrolysis by 0.5 microM lisinopril or captopril. Abz-FRK(Dnp)P-OH cleavage by ACE was monitored in rat lung, kidney, heart, and liver homogenates in the presence of a cocktail of inhibitors containing trans-epoxy-succinyl-L-leucylamido-(4-guanido)-butene, pepstatin, phenyl-methylsulfonyl fluoride, N-tosyl-L-phenylalanyl-chloromethyl ketone, and N-tosyl-lysyl-chloromethyl ketone to prevent undesirable hydrolysis. ACE activity in lung, heart and kidney homogenates, but not in liver homogenates, was completely abolished by 0.5 microM lisinopril or captopril. The advantages of the method are the procedural simplicity and the high sensitivity providing a rapid assay for ACE determinations.

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A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

Cited by 2 publications

References 17 publications

Diagnostic Utility of CD10 in Benign and Malignant Extrahepatic Bile Duct Lesions

A continuous fluorescent assay for the determination of plasma and tissue angiotensin I-converting enzyme activity

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