A Lactobacillus nifS-like gene suppresses an Escherichia coli transaminase B mutation

Yeast as a navigational aid in genome analysis

“…S. cerevisiae does not fix N 2 yet the nifS homologue is an essential gene. Similar genes have now been found in a number of other bacteria (Leong-Morgenthaler et al .,1994; Sun & Setlow, 1993; Mehta & Christen, 1993), none of which fix nitrogen, and experimental and informatic analyses (Leong-Morgenthaler et al ., 1994; Ouzounis & Sander, 1993) suggest that they encode a class of transaminases that use pyridoxal phosphate as a co-factor. In the nitrogen-fixing bacteria, the nifS gene product is now known to be responsible for the insertion of the sulphur into the Fe-S centre of the nitrogenase, again using pyridoxal phosphate as a co-factor (Zheng et al ., 1993).…”

Yeast as a navigational aid in genome analysis

“…S. cerevisiae does not fix N 2 yet the nifS homologue is an essential gene. Similar genes have now been found in a number of other bacteria (Leong-Morgenthaler et al .,1994; Sun & Setlow, 1993; Mehta & Christen, 1993), none of which fix nitrogen, and experimental and informatic analyses (Leong-Morgenthaler et al ., 1994; Ouzounis & Sander, 1993) suggest that they encode a class of transaminases that use pyridoxal phosphate as a co-factor. In the nitrogen-fixing bacteria, the nifS gene product is now known to be responsible for the insertion of the sulphur into the Fe-S centre of the nitrogenase, again using pyridoxal phosphate as a co-factor (Zheng et al ., 1993).…”

IV. Yeast sequencing reports. New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, Found on a 12·5 kbp fragment of chromosome IV of Saccharomyces cerevisiae

“…vinelandii NifS was demonstrated to be a pyridoxal phosphate-dependent cysteine desulfurase that participates in the biosynthesis of the nitrogenase metalloclusters (Zheng et al, 1993), but NifS proteins also exhibit remarkable sequence similarity to pyridoxal phosphate-dependent enzymes from non-nitrogen-fixing organisms. In general, this group of enzymes mediates specific chemical reactions common to diverse metabolic pathways (Leong-Morgenthaler et al, 1994). The enzymatic functions of Nif proteins found so far and the presence of sequence similarities with proteins from non-nitrogen-fixing organisms (e.g.…”

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17).…”

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17). In addition, a gene homologous to the Anabaena nifS has recently been identified in the budding yeast Saccharomyces cerevisiae (24).…”

Yeast as a navigational aid in genome analysis

“…S. cerevisiae does not fix N 2 yet the nifS homologue is an essential gene. Similar genes have now been found in a number of other bacteria (Leong-Morgenthaler et al .,1994; Sun & Setlow, 1993; Mehta & Christen, 1993), none of which fix nitrogen, and experimental and informatic analyses (Leong-Morgenthaler et al ., 1994; Ouzounis & Sander, 1993) suggest that they encode a class of transaminases that use pyridoxal phosphate as a co-factor. In the nitrogen-fixing bacteria, the nifS gene product is now known to be responsible for the insertion of the sulphur into the Fe-S centre of the nitrogenase, again using pyridoxal phosphate as a co-factor (Zheng et al ., 1993).…”

IV. Yeast sequencing reports. New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, Found on a 12·5 kbp fragment of chromosome IV of Saccharomyces cerevisiae

“…vinelandii NifS was demonstrated to be a pyridoxal phosphate-dependent cysteine desulfurase that participates in the biosynthesis of the nitrogenase metalloclusters (Zheng et al, 1993), but NifS proteins also exhibit remarkable sequence similarity to pyridoxal phosphate-dependent enzymes from non-nitrogen-fixing organisms. In general, this group of enzymes mediates specific chemical reactions common to diverse metabolic pathways (Leong-Morgenthaler et al, 1994). The enzymatic functions of Nif proteins found so far and the presence of sequence similarities with proteins from non-nitrogen-fixing organisms (e.g.…”

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17).…”

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17). In addition, a gene homologous to the Anabaena nifS has recently been identified in the budding yeast Saccharomyces cerevisiae (24).…”

Yeast as a navigational aid in genome analysis

“…S. cerevisiae does not fix N 2 yet the nifS homologue is an essential gene. Similar genes have now been found in a number of other bacteria (Leong-Morgenthaler et al .,1994; Sun & Setlow, 1993; Mehta & Christen, 1993), none of which fix nitrogen, and experimental and informatic analyses (Leong-Morgenthaler et al ., 1994; Ouzounis & Sander, 1993) suggest that they encode a class of transaminases that use pyridoxal phosphate as a co-factor. In the nitrogen-fixing bacteria, the nifS gene product is now known to be responsible for the insertion of the sulphur into the Fe-S centre of the nitrogenase, again using pyridoxal phosphate as a co-factor (Zheng et al ., 1993).…”

IV. Yeast sequencing reports. New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, Found on a 12·5 kbp fragment of chromosome IV of Saccharomyces cerevisiae

“…vinelandii NifS was demonstrated to be a pyridoxal phosphate-dependent cysteine desulfurase that participates in the biosynthesis of the nitrogenase metalloclusters (Zheng et al, 1993), but NifS proteins also exhibit remarkable sequence similarity to pyridoxal phosphate-dependent enzymes from non-nitrogen-fixing organisms. In general, this group of enzymes mediates specific chemical reactions common to diverse metabolic pathways (Leong-Morgenthaler et al, 1994). The enzymatic functions of Nif proteins found so far and the presence of sequence similarities with proteins from non-nitrogen-fixing organisms (e.g.…”

Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17).…”

“…The nifS gene in L. bulganicus (which also does not fix nitrogen) was identified by its ability to suppress an E. coli ilvE mutation (17). While the mechanism of this suppression by NifS is not completely clear, it might be due to a weak transaminase activity of NifS (17). In addition, a gene homologous to the Anabaena nifS has recently been identified in the budding yeast Saccharomyces cerevisiae (24).…”