A label-free and enzyme-free fluorescent aptasensor for amplified detection of kanamycin in milk sample based on target-triggered catalytic hairpin assembly

Cui, Wanling; Hu, Guodong; Lv, Enguang; Li, Chonghui; Wang, Zhenxing; Li, Qiang; Qian, Zhaohong; Wang, Jihua; Xu, Shicai; Wang, Rui

doi:10.1016/j.foodcont.2021.108654

Cited by 19 publications

(5 citation statements)

References 45 publications

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“…This MEF and PRET dual model nanoplatform could be flexibly controlled by temperature and has great potential in tracking and observing temperature-dependent transitions in biological and/or chemical processes. In future, by using the suitable nucleic acid strand and the editing complementary pairing principle, 30 this reversible PERT and MEF strategy may be applied to detect the molecular distance related events in living cell systems.…”

Section: Discussionmentioning

confidence: 99%

Temperature-sensitive metal-enhanced fluorescence and plasmon resonance energy transfer

Zhang,

Wang,

et al. 2024

Anal. Methods

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Section: Discussionmentioning

confidence: 99%

Temperature-sensitive metal-enhanced fluorescence and plasmon resonance energy transfer

Zhang,

Wang,

et al. 2024

Anal. Methods

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“…In order to facilitate testing, milk samples need to be processed. 29 First, dilute 10 ml of milk sample with 10 ml of ultra-pure water. The protein was precipitated with 20% acetic acid and the pH of the diluted milk sample was adjusted to 4.6.…”

Section: Methodsmentioning

confidence: 99%

A enzyme-free fluorescence quenching sensor for amplified detection of kanamycin in milk based on competitive triggering strategies

Bao,

Sang,

Yan

et al. 2024

RSC Adv.

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“…Then, adjust the pH to 7.0. Therewith, the sample was filtered by 0.22 μm filter membrane [ 48 ]. Finally, different concentrations of CAP (10 pM, 1 nM, 10 nM, and 100 nM) were added to the milk samples and detected by the proposed electrochemical aptasensor.…”

Section: Methodsmentioning

confidence: 99%

Few Layer Ti3C2 MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk

Li,

Xiong,

Zhao

et al. 2023

Molecules

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Quantitative detection of veterinary drug residues in animal-derived food is of great significance. In this work, a simple and label-free electrochemical aptasensor for the highly sensitive detection of chloramphenicol (CAP) in milk was successfully developed based on a new biosensing method, where the single- or few-layer Ti3C2 MXene nanosheets functionalized via the specific aptamer by self-assembly were used as electrode modifiers for a glassy carbon electrode (aptamer/Ti3C2 MXene/GCE). Differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscope (AFM), and so on were utilized for electrochemical and morphological characterization. Under the optimized conditions, the constructed aptasensor exhibited excellent performance with a wider linearity to CAP in the range from 10 fM to 1 μM and a low detection limit of 1 fM. Aptamer/Ti3C2 MXene/GCE demonstrated remarkable selectivity over other potentially interfering antibiotics, as well as exceptional reproducibility and stability. In addition, the aptasensor was successfully applied to determine CAP in milk with acceptable recovery values of 96.13% to 108.15% and relative standard deviations below 9%. Therefore, the proposed electrochemical aptasensor is an excellent alternative for determining CAP in food samples.

show abstract

A label-free and enzyme-free fluorescent aptasensor for amplified detection of kanamycin in milk sample based on target-triggered catalytic hairpin assembly

Cited by 19 publications

References 45 publications

Temperature-sensitive metal-enhanced fluorescence and plasmon resonance energy transfer

Temperature-sensitive metal-enhanced fluorescence and plasmon resonance energy transfer

A enzyme-free fluorescence quenching sensor for amplified detection of kanamycin in milk based on competitive triggering strategies

Few Layer Ti3C2 MXene-Based Label-Free Aptasensor for Ultrasensitive Determination of Chloramphenicol in Milk

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