Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.03.014 View full text
Hao Jiang, Huiming Yuan, Yu Liang, Simin Xia, Qun Zhao, Qi Wu, Lihua Zhang, Zhen Liang, Yukui Zhang

Abstract: a b s t r a c tIn this work, a novel kind of N-vinyl-2-pyrrolidinone (NVP) modified poly acrylic ester microspheres was prepared, followed by trypsin immobilization to prepare a hydrophilic immobilized enzyme reactor (IMER), to achieve highly efficient protein digestion with low peptide residue. The nonspecific adsorption of peptides on such an IMER was evaluated by the in sequence digestion of bovine serum albumin (BSA) and myoglobin. Without NVP modification, both proteins could be identified after digestio…

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