A human-origin probiotic cocktail ameliorates aging-related leaky gut and inflammation via modulating the microbiota/taurine/tight junction axis

Ahmadi, Shokouh; Wang, Shaohua; Nagpal, Ravinder; Wang, Bo; Jain, Shalini; Razazan, Atefeh; Mishra, Sidharth Prasad; Zhu, Xuewei; Zhan, Wei; Kavanagh, Kylie; Yadav, Hariom

doi:10.1172/jci.insight.132055

Cited by 130 publications

(98 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increase in Enterococcus species in the gut has been linked to decreased adiposity as a result of increased energy expenditure (Quan et al, 2019). A recent study showed that a probiotic cocktail of Lactobacillus and Enterococcus prevented dietinduced inflammation and leaky gut by increasing bile acid hydrolase activity (Ahmadi et al, 2020). Overall, this data supports the independent roles of FGF15 and VSG in the modulation of gut microbiota and future studies will dissect these roles on metabolism and energy expenditure.…”

Section: Discussionmentioning

confidence: 63%

Intestinal-derived FGF15 preserves muscle and bone mass following sleeve gastrectomy

Bozadjieva-Kramer

Shin

Shao

et al. 2020

Preprint

View full text Add to dashboard Cite

Bariatric surgeries such as the Vertical Sleeve Gastrectomy (VSG) are invasive, but provide the most effective long-term metabolic improvements in obese and Type 2 diabetic patients. These powerful effects of manipulating the gastrointestinal tract point to an important role of gastrointestinal signals in regulating both energy balance and metabolism. To that end, we have used mouse models of VSG to identify key gut signals that mediate these beneficial effects. Preliminary data from our rodent model of VSG led us to hypothesize a potential role for the hormone Fibroblast-Growth Factor15/19 (mouse/human ortholog) which pharmacologically can regulate many aspects of energy homeostasis and glucose handling. FGF15 is expressed in ileal enterocytes of the small intestine and is released postprandially. Like many other gut hormones, postprandial plasma levels in humans and ileal FGF15 expression in mice increase after VSG. We generated intestinal-specific FGF15 knock out (VilCreERT2;Fgf15 f/f ) mice and controls, which were maintained on 60% high-fat diet. Interestingly, ablation of intestinal FGF15 in adult mice results in little change to body weight or glucose regulation when challenged with a high-fat diet. Unlike what we had predicted, intestinal-specific FGF15 knock out mice lost more weight after VSG and this was a result of increased lean tissue loss compared to control mice. Further, the loss of bone mineral density observed after VSG in control mice was increased in intestinal-specific FGF15 knock out mice. Finally the effect of VSG to reduce hepatic cholesterol was also absent in intestinal-specific FGF15 knock out mice. These data point to an important role for intestinal FGF15 to protect the organism from deleterious effects of rapid weight loss that occurs after VSG.

show abstract

Section: Discussionmentioning

confidence: 63%

Intestinal-derived FGF15 preserves muscle and bone mass following sleeve gastrectomy

Bozadjieva-Kramer

Shin

Shao

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Gut microbiome analysis . Gut microbiome was examined as per our previously described methods [ 66 , 67 , 68 , 69 , 70 ]. Briefly, bacterial genomic DNA from fecal specimens was extracted by using a Qiagen DNA Stool Mini Kit (Qiagen, Valencia, CA, USA) with a slight modification described previously [ 66 ].…”

Section: Methodsmentioning

confidence: 99%

Role of TRP Channels in Shaping the Gut Microbiome

et al. 2020

Self Cite

View full text Add to dashboard Cite

Transient receptor potential (TRP) channel family proteins are sensors for pain, which sense a variety of thermal and noxious chemicals. Sensory neurons innervating the gut abundantly express TRPA1 and TRPV1 channels and are in close proximity of gut microbes. Emerging evidence indicates a bi-directional gut–brain cross-talk in several entero-neuronal pathologies; however, the direct evidence of TRP channels interacting with gut microbial populations is lacking. Herein, we examine whether and how the knockout (KO) of TRPA1 and TRPV1 channels individually or combined TRPA1/V1 double-knockout (dKO) impacts the gut microbiome in mice. We detect distinct microbiome clusters among the three KO mouse models versus wild-type (WT) mice. All three TRP-KO models have reduced microbial diversity, harbor higher abundance of Bacteroidetes, and a reduced proportion of Firmicutes. Specifically distinct arrays in the KO models are determined mainly by S24-7, Bacteroidaceae, Clostridiales, Prevotellaceae, Helicobacteriaceae, Rikenellaceae, and Ruminococcaceae. A1KO mice have lower Prevotella, Desulfovibrio, Bacteroides, Helicobacter and higher Rikenellaceae and Tenericutes; V1KO mice demonstrate higher Ruminococcaceae, Lachnospiraceae, Ruminococcus, Desulfovibrio and Mucispirillum; and A1V1dKO mice exhibit higher Bacteroidetes, Bacteroides and S24-7 and lower Firmicutes, Ruminococcaceae, Oscillospira, Lactobacillus and Sutterella abundance. Furthermore, the abundance of taxa involved in biosynthesis of lipids and primary and secondary bile acids is higher while that of fatty acid biosynthesis-associated taxa is lower in all KO groups. To our knowledge, this is the first study demonstrating distinct gut microbiome signatures in TRPA1, V1 and dKO models and should facilitate prospective studies exploring novel diagnostic/ therapeutic modalities regarding the pathophysiology of TRP channel proteins.

show abstract

“…Gut microbiome analysis. Gut microbiome was examined as per our previously described methods [45][46][47][48][49]. Briefly, bacterial genomic DNA from fecal specimens was extracted by using Qiagen DNA Stool Mini Kit (Qiagen, Valencia, CA, United States) with slight modification described previously [45].…”

Section: Methodsmentioning

confidence: 99%

Role of TRP Channels in Shaping Gut Microbiome

Nagpal¹,

Mishra²,

Deep³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Transient receptor potential (TRP) channel family proteins are sensors for pain, which sense variety of thermal and noxious chemicals. Sensory neurons innervating the gut abundantly express TRPA1 and TRPV1 channels and are in close proximity of gut microbes. Emerging evidence indicates a bi-directional gut-brain cross-talk in several entero-neuronal pathologies; however, the direct evidence of TRP channels interacting with gut microbial populations is lacking. Herein, we examine whether and how the knockout (KO) of TRPA1 and TRPV1 channels individually or combined TRPA1/V1 double-knockout (dKO) impacts the gut microbiome in mice. We detect distinct microbiome clusters among the three KO mouse models versus wild-type (WT) mice. All three TRP-KO models have reduced microbial diversity, harbor higher abundance of Bacteroidetes, and reduced proportion of Firmicutes. Specifically distinct arrays in the KO models are determined mainly by S24-7, Bacteroidaceae, Clostridiales, Prevotellaceae, Helicobacteriaceae, Rikenellaceae, and Ruminococcaceae. A1KO mice have lower Prevotella, Desulfovibrio, Bacteroides, Helicobacter and higher Rikenellaceae and Tenericutes; V1KO mice demonstrate higher Ruminococcaceae, Lachnospiraceae, Ruminococcus, Desulfovibrio and Mucispirillum; while A1V1dKO mice exhibit higher Bacteroidetes, Bacteroides and S24-7 and lower Firmicutes, Ruminococcaceae, Oscillospira, Lactobacillus and Sutterella abundance. Also, the abundance of taxa involved in biosynthesis of lipids and primary and secondary bile acids is higher while that of fatty acid biosynthesis-associated taxa is lower in all KO groups. To our knowledge, this is the first study demonstrating distinct gut microbiome signatures in TRPA1, V1 and dKO models and should facilitate prospective studies exploring novel diagnostic/ therapeutic modalities regarding the pathophysiology of TRP channel proteins.

show abstract

A human-origin probiotic cocktail ameliorates aging-related leaky gut and inflammation via modulating the microbiota/taurine/tight junction axis

Cited by 130 publications

References 87 publications

Intestinal-derived FGF15 preserves muscle and bone mass following sleeve gastrectomy

Intestinal-derived FGF15 preserves muscle and bone mass following sleeve gastrectomy

Role of TRP Channels in Shaping the Gut Microbiome

Role of TRP Channels in Shaping Gut Microbiome

Contact Info

Product

Resources

About