A HPLC Method for Determination of Ursolic Acid and Betulinic Acids from their Methanolic Extracts of Vitex Negundo Linn

Taralkar, S. V.; Chattopadhyay, Sudip

doi:10.4172/2155-9872.1000134

Cited by 32 publications

(18 citation statements)

References 10 publications

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“…The absorbance of curcumin was detected at a wavelength of 425 nm with a 0.8 mL/min flow rate. Similarly, RosA or UrsA were also extracted by the same method as the one used for curcumin, whereas there were slight changes in the detection: RosA and UrsA absorbances were detected at the wavelengths of 280 and 210 nm, respectively, with a flow rate of 1.0 mL/min [22,23]. The sample solution (20 μL) was injected to HPLC and analyzed until 10 min.…”

Section: Methodsmentioning

confidence: 99%

Potential Albumin-Based Antioxidant Nanoformulations for Ocular Protection against Oxidative Stress

et al. 2019

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Amongst various drug administration methods, ophthalmic drug delivery has been a useful way for the treatment of eye-related diseases. However, therapeutic efficacy of ocular therapy for anterior or posterior eye segments through topical administration is considerably challenged by the number of anatomical and physiological barriers in the eyes affecting ocular bioavailability. In this respect, advanced biocompatible nanoformulations make it possible to improve drug delivery to the target sites and enhance ocular bioavailability of ophthalmic medicines. Various ocular diseases have been reported to be related to oxidative stresses in tissues, and polyphenolic compounds have been known for their antioxidant activities in various tissues, including the eyes. Despite drug efficacy, poor water solubility and intrinsic color of the compounds limit the drug’s inclusion into the development of ocular medicine. In the present study, we investigated the antioxidant protectant efficacy of rosmarinic or ursolic acid in the retinal epithelial cells, as compared to those of curcumin, by forming nanospheres with bovine serum albumin. Our results demonstrate that antioxidant-containing nanoformulations provide a significantly higher drug solubility and decreased ROS (reactive oxygen species) production in the retinal epithelial cells. Finally, we also found that albumin-based nanoformulations could improve bioavailability and increase antioxidant activity of rosmarinic or ursolic acid in the retina to be applied as efficient ocular protectant.

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Section: Methodsmentioning

confidence: 99%

Potential Albumin-Based Antioxidant Nanoformulations for Ocular Protection against Oxidative Stress

et al. 2019

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“…Because of the low volatility of the ana lytes, their gas chromatographic separation is per formed at elevated temperatures (above 300°C) or after preliminary derivatization [8], which makes the analysis laborious and long. Among the versions of liq uid chromatography, the widest application was found by reversed phase HPLC with spectrophotometric detection [9][10][11]. Because of the absence of chro mophore groups intensely absorbing in the near UV spectrum region in triterpenoid molecules, they are detected at the wavelengths 205-210 nm against intense solvent absorption, which hinders the applica tion of gradient elution and deteriorates the reproduc ibility of the results of analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of triterpenoids from birch bark by liquid chromatography-tandem mass spectrometry

2014

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Pentacyclic triterpenoids from birch bark are of great interest as biologically active substances and raw materials for producing a wide range of drugs. In this work, a method for the highly sensitive determina tion of betulin, betulinic acid, lupeol, and erythrodiol in plant extracts based on a combination of high per formance liquid chromatography with tandem mass spectrometric detection is proposed. Mass spectra under the conditions of atmospheric pressure chemical ionization and collision induced dissociation spectra of analytes are obtained. The conditions for mass spectrometric detection in the multiple reaction monitoring mode are optimized. The calculated detection limits are in the range 0.7-1.8 µg/L.

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“…[53] Plantago major L Phytochemicals isolated from Plantago major contain five classes of biologically active compounds: aucubin [80] H HO H COOH Figure 3 Ursolic acid. [81] ( Figure 4) belongs to class iridoid glycoside, baicalein, baicalin and luteolin are derivatives of flavons ( Figure 5), and vanillic acid ( Figure 6) belongs to benzoic compounds and derivatives of cinnamic acid ( Figure 7) like caffeic acid, chlorogenic acid, ferulic acid, p-coumaric acid, triterpenes ursolic acid ( Figure 3) and oleanolic acid ( Figure 8). Invitro antiviral activity of these compounds has been checked against three types of human adenovirus at different concentrations.…”

Section: Justicia Adhatoda Lmentioning

confidence: 99%

A review on antiviral activity of the Himalayan medicinal plants traditionally used to treat bronchitis and related symptoms

Amber

Adnan

Tariq

et al. 2016

Journal of Pharmacy and Pharmacology

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Objectives Bronchitis is a common respiratory tract infection of humans mainly caused by influenza virus, rhinovirus, adenovirus, coronavirus and respiratory syncytial virus. The aim of this review was to gather fragmented literature on ethnomedicinal plants used against bronchitis in the Himalayan region and their in-vitro validation against bronchitis causing viral pathogens. Key findings Present review contains ethnomedicines of total 55 plants from different countries of the Himalayas. Most of the literature reported was from India followed by Pakistan, China and Nepal. Familiarly used plant families for bronchitis treatment in the Himalayan region were Leguminosae (six plants) and Lamiaceae (five plants). Leaves and roots were the most common parts used in ethnomedicines against bronchitis. Of these 55 plants, only six plants have been studied in vitro against viral pathogens causing bronchitis. Different compounds like monoterpenoids, flavonoids, triterpenoids, iridoid glycosides, sesquiterpenes, benzoic and phenolic compounds were reportedly isolated from these plant extracts having strong antiviral potential. Summary The Himalayan regions possess variety of ethnomedicinal plants used against respiratory diseases, but still there are only few studies related with their in-vitro validation. We invite the attention of researchers for detailed ethnopharmacological and phytochemical studies on unexplored plants used to treat bronchitis for the development of novel antiviral drugs.

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A HPLC Method for Determination of Ursolic Acid and Betulinic Acids from their Methanolic Extracts of Vitex Negundo Linn

Cited by 32 publications

References 10 publications

Potential Albumin-Based Antioxidant Nanoformulations for Ocular Protection against Oxidative Stress

Potential Albumin-Based Antioxidant Nanoformulations for Ocular Protection against Oxidative Stress

Determination of triterpenoids from birch bark by liquid chromatography-tandem mass spectrometry

A review on antiviral activity of the Himalayan medicinal plants traditionally used to treat bronchitis and related symptoms

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