The guqin, a seven-string Chinese zither invented three thousand years ago, is made of natural organic materials. For optimal acoustics, ancient makers used highly selected materials and experimented with complex manipulations. The sound box was carved from aged wood and artificially aged wood. The urushi lacquer contained organic additives, inorganic additives, and volume-extending particles. The silk string was coated with collagen, starch, and herbal extracts. Here, we review guqin-making materials and methods in ancient records and compare them to current chemical knowledge and analytical evidence. Understanding the technical art history of Chinese guqin is critical for its conservation and revival in the 21st century.