A Highly Specific BODIPY-Based Fluorescent Probe for the Detection of Hypochlorous Acid

Abstract

A fluorescent probe, HKOCl-1, has been successfully developed for the detection of hypochlorous acid on the basis of a specific reaction with p-methoxyphenol. The formation of HOCl has been successfully detected not only in an abiotic system but also in an enzymatic system (myeloperoxidase/H2O2/Cl(-) system) and in living macrophage cells upon stimulation. This new probe might be used as an efficient tool for probing the roles HOCl plays in biological systems.

A BODIPY aldoxime-based chemodosimeter for highly selective and rapid detection of hypochlorous acid

“…6 Functional groups, sensitive to hypochlorite oxidation, such as p-methoxyphenol, 7 dibenzoylhydrazine, 6f thiol, 6d selenide, 6a-c oxime 8 and p-alkoxyaniline 9 have been extensively utilized in the probe design. Some of these probes, however, show pH dependency and function only under basic conditions (pH > 9.0), which is an undesired feature for cell imaging studies.…”

The Fluorescein-derived Dye Aminophenyl Fluorescein Is a Suitable Tool to Detect Hypobromous Acid (HOBr)-producing Activity in Eosinophils

“…Besides APF/HPF, different other fluorescent probes have been tested to specifically detect the halogenating activity of MPO, including sulfonaphthoaminophenyl fluorescein, tetramethylrhodamine, and p-methoxyphenol-coupled boron dipyrromethene (67)(68)(69). However some of these dyes are only partially applicable to flow cytometry and/or are not commercially available.…”

A novel fluorescent probe for imaging the process of HOCl oxidation and Cys/Hcy reduction in living cells

“…A control molecule CMOS-2 was also prepared by 3-acetyl-7-diethylaminocoumarin (CMAC) similarly. The structure of all these compounds have been convinced by 1 H NMR, 13 C NMR, and HR-MS (see ESI †).…”