A Highly Sensitive, Dual-Readout Assay Based on Gold Nanoparticles for Organophosphorus and Carbamate Pesticides

Liu, Dingbin; Chen, Wenwen; Wei, Jinhua; Li, Xuebing; Wang, Zhuo; Jiang, Xingyu

doi:10.1021/ac300545p

Cited by 397 publications

(173 citation statements)

References 49 publications

(61 reference statements)

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“…These impurities are far more potent anti-cholinesterase agents, which inhibit free and immobilized AChE in a concentration-dependent manner [32,33]. This phenomenon was also observed by others [34,35]. With increasing the malathion concentration, the produced current of ATCl on the AChE-MWCNT-CA-NPG electrode decreases (Fig.…”

Section: Calibration Curve For Malathion Determinationsupporting

confidence: 70%

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Ding

Zhang

Jia

et al. 2014

Sensors and Actuators B: Chemical

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a b s t r a c tAn electrochemical sensing platform based on assembly of carbon nanotubes on a nanoporous gold electrode is described for highly sensitive detection of organophosphate pesticides. The nanoporous gold film (NPG) electrode is fabricated by an alloying/dealloying process, which possess high electroactive surface area and is an excellent substrate for sensor design. The NPG functionalized with cysteamine allows the immobilization of carbon nanotubes on the electrode with the self-assembly technique. The carboxylated carbon nanotubes are further linkered with acetylcholinesterase (AChE) for amperometric sensing of pesticides. The immobilized AChE, as a model, shows excellent activity to its substrate and allows a quantitative measurement of organophosphate pesticides. Under the optimal experimental conditions, the inhibition of malathion is proportional to its concentration in the range of 0.001-0.5 g mL −1 with a detection limit of 0.5 ng mL −1 . The proposed method shows good reproducibility and high stability, which provides a new avenue for electrochemical biosensor design.

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Section: Calibration Curve For Malathion Determinationsupporting

confidence: 70%

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Ding

Zhang

Jia

et al. 2014

Sensors and Actuators B: Chemical

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“…12−26 However, the establishment of colorimetric assays for PFCs has not been reported yet. Actually, the applications of colorimetric assays based on Au NPs for environmental pollutants are scarce, and only very few colorimetric assays have been designed for TNT, 27,28 organophosphorus and carbamate pesticides, 29 and phthalates. 30 Selective isolation of fluorinated targets from convoluted mixtures has become increasingly popular using liquid−liquid separations, solid−liquid separations (such as fluorous solidphase extraction), and fluorous chromatography.…”

mentioning

confidence: 99%

Sensitive Colorimetric Visualization of Perfluorinated Compounds Using Poly(ethylene glycol) and Perfluorinated Thiols Modified Gold Nanoparticles

et al. 2014

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In this work, we have developed a novel sensing strategy employing mixed poly(ethylene glycol)-terminated (PEG-thiols) and perfluoroalkyl-terminated (F-thiols) alkanethiols modified gold nanoparticles (Au@PEG-F NPs) as a probe to detect perfluorinated compounds (PFCs) from water samples. PEG-thiols with high density and long carbon chains make the Au NPs probe well-dispersed in solution and stable even in high concentration of salt solution; F-thiols provide specific fluorous−fluorous interactions to PFCs, which results in adsorption of PFCs on Au@PEG-F NPs. The adsorbed PFCs cause the aggregation of Au@PEG-F NPs probes and thus induce the insolubility of probes and precipitation directly from reaction solution due to the superhydrophobicity of perfluorocarbon monolayers, leading to color and absorbance response of the assay to PFCs. The preparation of the Au@PEG-F NPs probe is very simple, and the colorimetric assay based on this mechanism for the detection of PFCs is selective and convenient. Combined with UV−vis spectrophotometry, the assay demonstrates good sensitivities to PFCs with wide linear range. In the designed concentration range, the response of the colorimetric assay to longchain PFCs (perfluoroalkyl chain ≥7) is discerned even as the concentration of these PFCs is as low as 10 μg L −1 . This low-cost and sensitive assay shows great potential to measure total PFCs in water samples. To the best of our knowledge, this is the first application of the specific fluorous−fluorous interactions and Au NPs based probes for colorimetric recognition for PFCs.

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“…Recently, the organic energy acceptor was replaced with gold nanoparticles to render the nanometal surface energy transfer (NSET) mechanism. 21,28 Compared to FRET, NSET has a higher energy transfer rate from the organic donor to the AuNPs acceptor and a longer quenching distance.…”

Section: -25mentioning

confidence: 99%

Highly Sensitive and Naked Eye Dual-readout Method for ʟ-Cysteine Detection Based on the NSET of Fluorophore Functionalized Gold Nanoparticles

Fu¹,

Liu²,

Wu³

et al. 2014

Bulletin of the Korean Chemical Society

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A simple, highly sensitive and selective method based on the rhodamine B-covered gold nanoparticle with dual-readout (colorimetric and fluorometric) detection for L-cysteine is proposed. A mechanism is that citratestabilized AuNPs were modified with RB by electrostatic interaction, which enables the nanometal surface energy transfer (NSET) from the RB to the AuNPs, quenching the fluorescence. In the presence of L-cysteine, it was used as a competitor in the NSET by the strongly Au-S bonding to release RB from the Au surface and recover the fluorescence, and the red-to-purple color change quickly, which was monitored simply by the naked eye. Under the optimum conditions, the detection limit is as low as 10 nM. The method possessed the advantages of simplicity, rapidity and sensitivity at the same time. The method was also successfully applied to the determination of L-cysteine in human urine samples, and the results were satisfying.

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A Highly Sensitive, Dual-Readout Assay Based on Gold Nanoparticles for Organophosphorus and Carbamate Pesticides

Cited by 397 publications

References 49 publications

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Assembly of carbon nanotubes on a nanoporous gold electrode for acetylcholinesterase biosensor design

Sensitive Colorimetric Visualization of Perfluorinated Compounds Using Poly(ethylene glycol) and Perfluorinated Thiols Modified Gold Nanoparticles

Highly Sensitive and Naked Eye Dual-readout Method for ʟ-Cysteine Detection Based on the NSET of Fluorophore Functionalized Gold Nanoparticles

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