A highly sensitive and selective high pressure liquid chromatography with tandem mass spectrometry (HPLC/MS-MS) method for the direct peptide reactivity assay (DPRA)

Zhang, Fagen; Erskine, Tim; Klapacz, Joanna; Settivari, Raja S.; Marty, Sue

doi:10.1016/j.vascn.2018.07.004

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…BMIM, BMPY, and NBuPY, which were also classified by the prediction model as nonsensitizers, did not deplete the cysteine peptide but did alter the retention time of a portion of the lysine peptide resulting in a split peak. Complications, such as noncovalent interactions, weak binding, or similarity in column retention times between the test article and the peptide in the HPLC/UV‐based DPRA can lead to interference with the peptide peak and false prediction/classification of test chemicals (Zhang et al, 2018). The split peak observed with all of the butylated ILs in this study suggests a weak interaction with the lysine peptide, as the proportion of peptide impacted increased with incubation time.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of skin sensitization induced by four ionic liquids

Frawley

Germolec

Johnson³

et al. 2021

J of Applied Toxicology

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Ionic liquids (ILs) are synthetic solvents used as replacements for volatile organic solvents. Human exposure occurs through dermal or oral routes. In rodents, several ILs were reported to induce dermal toxicity, irritation, and sensitization. Due to the potential for occupational exposure, and industrial use as nonvolatile solvents, 1‐ethyl‐3‐methylimidazolium chloride (EMIM, 6.25% to 50% v/v), 1‐butyl‐3‐methylimidazolium chloride (BMIM, 3.12% to 12.5% v/v), 1‐butyl‐1‐methylpyrrolidinium chloride (BMPY, 0.825% to 6.25% v/v), and N‐butylpyridinium chloride (NBuPY, 0.825% to 12.5% v/v) were nominated to the National Toxicology Program and evaluated for skin sensitization. The test compound was applied to the ears of female BALB/c mice daily for 3 days in a primary irritancy (IRR)/local lymph node assay (LLNA). Sensitization was assessed in vitro in the direct peptide reactivity assay (DPRA), KeratinoSens™ assay, and human cell line activation test (h‐CLAT). In the LLNA, the butylated ILs, BMIM, and BMPY were more potent than NBuPY (butylated) or EMIM (ethylated), which was neither an irritant nor a sensitizer. NBuPY induced skin irritation in vivo at ≥3.12% (p ≤ 0.01), and sensitization in vitro in the KeratinoSens™ assay and h‐CLAT, but was negative for sensitization in vivo and in the DPRA. Although SI3 was not achieved, dermal treatment with 12.5% BMIM or 6.25% BMPY increased (p ≤ 0.01) lymph node cell proliferation in the LLNA. In vitro, BMIM was positive for sensitization in the h‐CLAT, and BMPY was positive in the h‐CLAT and KeratinoSens™ assay; both were negative in the DPRA. Integrated data analyses, weighted toward in vivo data, suggested that BMIM and BMPY may induce weak to mild sensitization.

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Section: Discussionmentioning

confidence: 99%

Evaluation of skin sensitization induced by four ionic liquids

Frawley

Germolec

Johnson³

et al. 2021

J of Applied Toxicology

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“…Similarly, several in chemico reactivity assays that can be used to assess the MIE in the skin sensitization AOP have been reported in the literature (Aptula et al, 2006;Natsch and Gfeller, 2008;Avonto et al, 2015;Nepal et al, 2018;Zhang et al, 2018). In the present study, we compared and evaluated the utility of two alternative in chemico approaches, DPRA and ADRA, to support the discrimination between skin sensitizers and non-sensitizers as well as photosensitizers.…”

Section: Discussionmentioning

confidence: 96%

Comparison of in chemico skin sensitization methods and development of an in chemico skin photosensitization assay

Patel¹

2019

ALTEX

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“…Direct peptide reactivity assay is considered an "in chemico" assay for skin sensitization testing, measuring the phenomenon of haptenation, a process whereby small molecules turn into immunogenic after binding to larger ones, a consequence of the reaction of the ACI with certain proteins (aminoacids residues) [64]. In order to improve sensibility and selectivity for accurately identifying the sensitization potential of some ACIs, another study upgraded the analytical domains of the assay by developing and validating a high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC/MS-MS)-based direct peptide reaction (DPR) assay [65].…”

Section: General Requirements For the Assessment Of Toxicological Datamentioning

confidence: 99%

Safety Assessment of Nanomaterials in Cosmetics: Focus on Dermal and Hair Dyes Products

Coimbra

Sousa-Oliveira²,

Ferreira-Faria³

et al. 2022

Cosmetics

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Nanomaterials use in cosmetics is markedly enhancing, so their exposure and toxicity are important parameters to consider for their risk assessment. This review article provides an overview of the active cosmetic ingredients used for cosmetic application, including dermal cosmetics and also hair dye cosmetics, as well as their safety assessment, enriched with a compilation of the safety assessment tests available to evaluate the different types of toxicity. In fact, despite the increase in research and the number of papers published in the field of nanotechnology, the related safety assessment is still insufficient. To elucidate the possible effects that nanosized particles can have on living systems, more studies reproducing similar conditions to what happens in vivo should be conducted, particularly considering the complex interactions of the biological systems and active cosmetic ingredients to achieve newer, safer, and more efficient nanomaterials. Toward this end, ecological issues and the toxicological pattern should also be a study target.

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A highly sensitive and selective high pressure liquid chromatography with tandem mass spectrometry (HPLC/MS-MS) method for the direct peptide reactivity assay (DPRA)

Cited by 9 publications

References 40 publications

Evaluation of skin sensitization induced by four ionic liquids

Evaluation of skin sensitization induced by four ionic liquids

Comparison of in chemico skin sensitization methods and development of an in chemico skin photosensitization assay

Safety Assessment of Nanomaterials in Cosmetics: Focus on Dermal and Hair Dyes Products

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