A High Throughput System for Long Term Application of Intermittent Cyclic Hydrostatic Pressure on Cells in Culture

Abstract

The process of bone remodeling is governed by mechanical stresses and strains. Studies on the effects of mechanical stimulation on cell response are often difficult to compare as the nature of the stimuli and differences in parameters applied vary greatly. Experimental systems for the investigation of mechanical stimuli are mostly limited in throughput or flexibility and often the sum of several stimuli is applied. In this work, a flexible system that allows the investigation of cell response to isolated intermittent cyclic hydrostatic pressure (icHP) on a high throughput level is shown. Human bone derived cells were cultivated with or without mechanical stimulus in the presence or absence of chemical cues triggering osteogenesis for 7-10 days. Cell proliferation and osteogenic differentiation were evaluated by cell counting and immunohistochemical staining for bone alkaline phosphatase as well as collagen 1, respectively. In either medium, both cell proliferation and level of differentiation were increased when the cultures were mechanically stimulated. These initial results therefore qualify the present system for studies on the effects of isolated icHP on cell fate and encourage further investigations on the details behind the observed effects.

Poromicromechanics reveals that physiological bone strains induce osteocyte-stimulating lacunar pressure

“…( 2010 ) MLO-Y4 osteocyte-like cells (i.e., deriving from cells extracted from transgenic mice) 68 kPa 0.5 Hz (triangular wave form), for 1 or 2 h Decreased osteocyte apoptosis, increase in intracellular calcium (after 40 s, may be related to osteoblast activity), of RANKL/OPG ratio (after 2 h), and of COX-2 mRNA level (after 1 h) Rottmar et al. ( 2011 ) Human bone- derived cells, from the hip marrow 1–11 kPa 30 min stimulation, 7 h, 30 min break Increased osteogenic differentiation and proliferation Henstock et al. ( 2013 ) Cells contained in whole femurs of chick foetuses 0–279 kPa 0.0001–2 Hz Increased volume of diaphysial collar …”

A Comprehensive Review on the Role of Various Materials in the Osteogenic Differentiation of Mesenchymal Stem Cells with a Special Focus on the Association of Heat Shock Proteins and Nanoparticles

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