2015
DOI: 10.1074/mcp.o115.052209
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Abstract: Antibodies are key reagents in biology and medicine, but commercial sources are rarely recombinant and thus do not provide a permanent and renewable resource. Here, we describe an industrialized platform to generate antigens and validated recombinant antibodies for 346 transcription factors (TFs) and 211 epigenetic antigens. We describe an optimized automated phage display and antigen expression pipeline that in aggregate produced about 3000 sequenced Fragment antigen-binding domain that had high affinity (typ… Show more

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Cited by 101 publications
(126 citation statements)
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“…antibody selections (Hornsby et al 2015;Marcon et al 2015), annotated domains or full-length proteins have previously been the most commonly used antigens for synthetic antibody production (Schofield et al 2007;Colwill and Graslund 2011;Hornsby et al 2015;Huang et al 2015). However, although this provides a straightforward approach to choosing antigens, for our purposes, we sought to exclude annotated RNA-binding domains as antigens in order to prevent the production of antibodies that would disrupt RBP-RNA interactions and, for 75% of the RNA-binding RNP complex proteins, were left with no other annotated domains from which to choose.…”
Section: Cdr-l3 Cdr-h1 Cdr-h2mentioning
confidence: 99%
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“…antibody selections (Hornsby et al 2015;Marcon et al 2015), annotated domains or full-length proteins have previously been the most commonly used antigens for synthetic antibody production (Schofield et al 2007;Colwill and Graslund 2011;Hornsby et al 2015;Huang et al 2015). However, although this provides a straightforward approach to choosing antigens, for our purposes, we sought to exclude annotated RNA-binding domains as antigens in order to prevent the production of antibodies that would disrupt RBP-RNA interactions and, for 75% of the RNA-binding RNP complex proteins, were left with no other annotated domains from which to choose.…”
Section: Cdr-l3 Cdr-h1 Cdr-h2mentioning
confidence: 99%
“…In particular, we wanted to select regions that are likely to fold independently into stable structures, since such regions are required to optimize the chances of yielding antibodies by phage display approaches (Hornsby et al 2015). First, we searched for annotated domains since these have served as effective antigens for synthetic antibody production in other studies (Colwill and Graslund 2011;Huang et al 2015).…”
Section: Computationally Guided Identification Of Antigenic Protein Rmentioning
confidence: 99%
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