A high-performance liquid chromatographic assay for determination of cisplatin in plasma, cancer cell, and tumor samples

Lopez-Flores, Angelica; Jurado, Rafael; Garcı́a-López, Patricia

doi:10.1016/j.vascn.2005.06.005

Cited by 54 publications

(53 citation statements)

References 17 publications

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“…The amount of cisplatin released and the uptake into the HeLa cells was determined using HPLC, as reported earlier (Lopez-Flores et al, 2005). The HeLa cells (1.0 × 10 6 cells/ well) were treated with CS-cisplatin-Fe 3 O 4 with a final concentration of 50 μg/mL and were incubated for 3, 6, or 12 h in a 5% CO 2 atmosphere at 37 o C. The untreated cells samples were incubated for the same period.…”

Section: In Vitro Release Of Cisplatin and Drug Uptakementioning

confidence: 99%

Chitosan-Coated Fe3O4 Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

Arum¹,

Oh²,

Kang³

et al. 2015

Fisheries and aquatic sciences

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A synthesis method for a chitosan-coated magnetic drug-delivery system of cisplatin is proposed. Here, cisplatin was conjugated to the surface of Magnetite (Fe 3 O 4 ) nanoparticles via a (3-Aminopropyl)-trimethoxysilane (APTS) coupling agent. To reduce the cytotoxic effect of cisplatin, the magnetic drug was then encapsulated in chitosan (CS-cisplatin-Fe 3 O 4 ) through the water/oil (W/O) emulsion method. The CS-cisplatin-Fe 3 O 4 nanoparticles were synthesized in a spherical shape with a diameter of 190 nm. The cytotoxicity assay was performed using HeLa cells. The cisplatin uptake of the cells was determined using High Performance Liquid Chromatography (HPLC) to calculate the drug content. The controlled release of cisplatin was demonstrated by regulating the dissolution and diffusion of the drug through the chitosan matrix.

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Section: In Vitro Release Of Cisplatin and Drug Uptakementioning

confidence: 99%

Chitosan-Coated Fe3O4 Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

Arum¹,

Oh²,

Kang³

et al. 2015

Fisheries and aquatic sciences

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“…21,[42][43][44][45] Those studies used DDTC for derivatization. The derivatization efficiency was higher than 80%.…”

Section: Pretreatmentmentioning

confidence: 99%

Quantitative determination of platinum derived from cisplatin in human plasma ultrafiltrate using derivatization with diethyldithiocarbamate and liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Tang¹,

Tang²,

Zhan³

et al. 2013

Anal. Methods

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a This paper describes a method for the determination of platinum derived from cisplatin in human plasma ultrafiltrate using liquid chromatography tandem mass spectrometry (LC-MS/MS). Cisplatin and the internal standard trans-diamminedichloropalladium(II) (TDPD) were chelated with diethyldithiocarbamate (DDTC) and separated with an isocratic elution on a C18 column. The method was validated with intra-/inter-batch accuracy and precision (relative standard deviation, RSD, %), matrix effect, specificity, limit of quantification (LOQ), linearity and stability. The linear range was 1.0-100.0 ng mL À1 and the LOQ was 1.0 ng mL À1. The intra-/inter-batch accuracy and precision were 92.2-111.0%/94.7-106.0% and 3.9-8.8%/2.1-9.3%, respectively. This sensitive and interference-free method can permit reliable and accurate analysis of platinum derived from cisplatin in human plasma ultrafiltrate.

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“…After that they were counted with a haemocytometer and lysed with buffer (Tris 100 mM, EDTA 5 mM, NaCl 200 mM, SDS 0.2%, at pH 8.0) for 3 h at 55˚C. The intracellular concentration of cisplatin was estimated by the High Performance Liquid Chromatographic (HPLC) method previously validated and reported (13). Briefly, the homogenate was ultrafiltered and derivatized with DDTC in 0.1 N NaOH.…”

Section: Intracellular Cisplatin Accumulation Hela and Caski Human Cmentioning

confidence: 99%

“…The homogenate was ultrafiltrated, derivatizated with 20 μl of DDTC and extracted with 160 μl of chloroform. Finally, 20 μl of the chloroform layer was injected into a chromatographic system and the cisplatin concentration was determined using a method previously validated and reported (13).…”

Section: Intracellular Cisplatin Accumulation Hela and Caski Human Cmentioning

confidence: 99%

Cisplatin cytotoxicity is increased by mifepristone in cervical carcinoma: An in vitro and in vivo study

Jurado

Lopez-Flores²,

Álvarez³

et al. 2009

Oncol Rep

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Abstract. We investigated the ability of mifepristone, an anti-progestin drug, to modulate the cytotoxic effect of cisplatin in two cervical cancer cell lines and in human xenograft cervical tumors. The effect of cisplatin alone or combined with mifepristone on cellular proliferation was studied with the XTT assay which use a tetrazolium dye {sodium3'-[1-(phenylamino-carbonyl)-3,4-tetrazolium],XTT}. Before and after treatment with mifepristone, the intracellular accumulation of cisplatin in cancer cells and tumors of mice was evaluated by HPLC. The expression of Bcl-2 and Bax genes was also assessed by a reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. In addition, single agents and combination treatment in vivo studies were performed with the xenograft cervical model. Tumor measurements were carried out weekly. Analysis of the data by the isobologram method shows a synergistic antiproliferative effect produced by the combination of mifepristone with cisplatin only in the HeLa cervical cancer cell line but not in CaSki cells. The effect of mifepristone on cytotoxicity of cisplatin could be mediated, at least partially, by an increase of intracellular cisplatin accumulation, but not by changes in Bcl-2/Bax gene relation expression in these cells. In vivo studies showed that the combination of these agents has a significant antitumor activity against HeLa xenograft tumors. Our results suggest that mifepristone can improve the efficacy of the antiproliferative effect of cisplatin in vitro and in vivo. This anti-hormonal drug therapy may be a useful candidate for further evaluation in combination with other antineoplastic drugs in the treatment of cancer, particularly with cisplatin.

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A high-performance liquid chromatographic assay for determination of cisplatin in plasma, cancer cell, and tumor samples

Cited by 54 publications

References 17 publications

Chitosan-Coated Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

Chitosan-Coated Fe<sub>3</sub>O<sub>4</sub> Magnetic Nanoparticles as Carrier of Cisplatin for Drug Delivery

Quantitative determination of platinum derived from cisplatin in human plasma ultrafiltrate using derivatization with diethyldithiocarbamate and liquid chromatography coupled with electrospray ionization tandem mass spectrometry

Cisplatin cytotoxicity is increased by mifepristone in cervical carcinoma: An in vitro and in vivo study

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