A high density RFLP linkage map of sugar beet

Halldén, Christer; Hjerdin, A.; Rading, I. M.; Fridlundh, B.; Johannisdottir, G.; Tuvesson, Stig; Åkesson, Christina; Säll, Torbjörn; Nilsson, Nils-Otto

doi:10.1139/g96-081

Cited by 47 publications

(23 citation statements)

References 20 publications

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“…Sugar beet is a true diploid member of the Amaranthaceae family and has a haploid chromosome number of nine (half that of quinoa). The total length of the several dense linkage maps reported in sugar beet range from 621 cM (Halldén et al 1996) to 815 cM (Barzen et al 1995), roughly half the size predicted for the total length (1,700 cM) of the quinoa genetic map and between 61% and 80% of the genetic map we report for quinoa (1,020 cM). Considering the probable allotetraploid origin of quinoa, a genetic map roughly twice the size of those for diploid members of the family is not unexpected.…”

Section: Linkage Analysismentioning

confidence: 47%

A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers

et al. 2004

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Quinoa ( Chenopodium quinoa Willd.) is an important seed crop for human consumption in the Andean region of South America. It is the primary staple in areas too arid or saline for the major cereal crops. The objective of this project was to build the first genetic linkage map of quinoa. Selection of the mapping population was based on a preliminary genetic similarity analysis of four potential mapping parents. Breeding lines 'Ku-2' and '0654', a Chilean lowland type and a Peruvian Altiplano type, respectively, showed a low similarity coefficient of 0.31 and were selected to form an F(2) mapping population. The genetic map is based on 80 F(2) individuals from this population and consists of 230 amplified length polymorphism (AFLP), 19 simple-sequence repeat (SSR), and six randomly amplified polymorphic DNA markers. The map spans 1,020 cM and contains 35 linkage groups with an average marker density of 4.0 cM per marker. Clustering of AFLP markers was not observed. Additionally, we report the primer sequences and map locations for 19 SSR markers that will be valuable tools for future quinoa genome analysis. This map provides a key starting point for genetic dissection of agronomically important characteristics of quinoa, including seed saponin content, grain yield, maturity, and resistance to disease, frost, and drought. Current efforts are geared towards the generation of more than 200 mapped SSR markers and the development of several recombinant-inbred mapping populations.

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Section: Linkage Analysismentioning

confidence: 47%

A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers

et al. 2004

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“…1). Other authors have reported a non-random distribution of markers-often due to centrally located clusters-in chickpea (Winter et al 2000), sugar beet (HalldØn et al 1996) or barley (Langridge et al 1995). Sometimes the apparently random marker distribution is due to a low number of markers; when more markers are added to the map, clusters became evident, such as SSRs in soybean (Akkaya et al 1995;Cregan et al 1999).…”

Section: Discussionmentioning

confidence: 99%

An intersubspecific genetic map of Lens

et al. 2003

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A Lens map was developed based on the segregational analysis of five kinds of molecular and morphological genetic markers in 113 F(2) plants obtained from a single hybrid of Lens culinaris ssp. culinaris x L. c. ssp. orientalis. A total of 200 markers were used on the F(2) population, including 71 RAPDs, 39 ISSRs, 83 AFLPs, two SSRs and five morphological loci. The AFLP technique generated more polymorphic markers than any of the others, although AFLP markers also showed the highest proportion (29.1%) of distorted segregation. At a LOD score of 3.0, 161 markers were grouped into ten linkage groups covering 2,172.4 cM, with an average distance between markers of 15.87 cM. There were six large groups with 12 or more markers each, and four small groups with two or three markers each. Thirty-nine markers were unlinked. A tendency for markers to cluster in the central regions of large linkage groups was observed. Likewise, clusters of AFLP, ISSR or RAPD markers were also observed in some linkage groups, although RAPD markers were more evenly spaced along the linkage groups. In addition, two SSR, three RAPD and one ISSR markers segregated as codominant. ISSR markers are valuable tools for Lens genetic mapping and they have a high potential in the generation of saturated Lens maps.

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“…From this examination, genotypic data from seven individuals were excluded due to an extensive amount of genotypic errors. Loci showing a skewed segregation were examined for their effects on linked markers (Hallden et al 1996).…”

Section: Linkage Analysismentioning

confidence: 99%

An AFLP and RFLP linkage map and quantitative trait locus (QTL) analysis of growth traits in Salix

2002

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A genetic linkage map of Salix (2n = 38), composed of 325 AFLP and 38 RFLP markers has been constructed. The map was based on a population ( n = 87) derived from a cross between the male hybrid clone "Björn" ( Salix viminalis x Salix schwerinii) and the female clone "78183" ( S. viminalis). Three hundred fifty seven AFLPs corresponding to DNA polymorphisms heterozygous in one parent and null in the other were scored. A total of 87 RFLP probes, most (83) derived from the Populus genome, yielded 39 and 11 polymorphic loci segregating in a 1:1 and 1:2:1 ratio respectively. Two maps, one for each parent, were constructed according to the "two-way pseudo-testcross" mapping strategy. The S. viminalis x S. schwerinii map (2,404 cM) included 217 markers and formed 26 major linkage groups while S. viminalis (1,844 cM) consisted of 146 markers placed on 18 major groups. In addition, eight and 14 additional minor linkage groups composed of less than four markers (doubles and triplets) were obtained in the S. viminalis x S. schwerinii and the S. viminalis maps, respectively. Both maps provided 70-80% genome coverage with an average density of markers of 14 cM. To investigate possible homologies between the parental maps, 20 AFLPs and 11 RFLPs segregating in 3:1 or 1:2:1 ratios were included in the linkage analysis. Eight linkage groups homologous between the two maps were detected. The present genetic map was used to identify quantitative trait loci (QTLs) affecting growth-related traits. Eleven QTLs were identified; seven QTLs for height growth, one QTL for stem diameter, one QTL for the height: diameter ratio, one QTL for the number of vegetative buds during flowering time and one QTL for the number of shoots. The estimated magnitude of the QTL effect ranged from 14 to 22% of the total phenotypic variance. One QTL associated with height growth and one affecting the height: diameter ratio were overlapping in the same marker interval with the QTL affecting stem diameter. QTL stability over years was estimated for traits measured in multiple years. Generally, QTLs were only significant in a single year although two QTLs for height growth were close to reaching the significance level in 2 consecutive years.

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A high density RFLP linkage map of sugar beet

Cited by 47 publications

References 20 publications

A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers

A genetic linkage map of quinoa (Chenopodium quinoa) based on AFLP, RAPD, and SSR markers

An intersubspecific genetic map of Lens

An AFLP and RFLP linkage map and quantitative trait locus (QTL) analysis of growth traits in Salix

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