A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development in<i>Arabidopsis</i>

Myouga, Fumiyoshi; Hosoda, Chieko; Umezawa, Taishi; Iizumi, Haruko; Kuromori, Takashi; Motohashi, Reiko; Shono, Yuriko; Nagata, Noriko; Ikeuchi, Masahiko; Shinozaki, Kazuo

doi:10.1105/tpc.108.061341

Cited by 279 publications

(292 citation statements)

References 76 publications

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“…Recent reports have revealed that mutations in several of the genes encoding TAC components cause deficiencies in PEP-dependent transcription (Pfalz et al, 2006;Garcia et al, 2008;Myouga et al, 2008). As shown in Figure 5, real-time RT-PCR and northern-blot hybridization showed that the expression pattern of plastid genes in ptac14-1 mutants resembles that observed in Drpo mutants (Serino and Maliga, 1998;Krause et al, 2000) and in mutants with lesions in PEP function (Hess et al, 1993;Allison et al, 1996;Hajdukiewicz et al, 1997;Chateigner-Boutin et al, 2008;Chi et al, 2008).…”

Section: Discussionmentioning

confidence: 88%

“…We detected the relative expression levels of chloroplast genes using quantitative realtime RT-PCR compared with those of the wild type. The psaB, psbA, psbB, petD, and rbcL genes were chosen as PEP-dependent genes (class I); rpoA, ycf2, and accD were chosen as NEP-dependent genes (class III); and rrn16, rrn23, atpB, and atpE were used as both PEP-and NEP-dependent genes (class II; ChateignerBoutin et al, 2008;Myouga et al, 2008;Yu et al, 2009a). Our results showed that the transcription level of class I genes in ptac14-1 was clearly decreased by 70% to 95%.…”

Section: Chloroplast Development Is Defective In Ptac14 Mutantsmentioning

confidence: 99%

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A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

et al. 2011

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The SET domain-containing protein, pTAC14, was previously identified as a component of the transcriptionally active chromosome (TAC) complexes. Here, we investigated the function of pTAC14 in the regulation of plastid-encoded bacterialtype RNA polymerase (PEP) activity and chloroplast development. The knockout of pTAC14 led to the blockage of thylakoid formation in Arabidopsis (Arabidopsis thaliana), and ptac14 was seedling lethal. Sequence and transcriptional analysis showed that pTAC14 encodes a specific protein in plants that is located in the chloroplast associated with the thylakoid and that its expression depends on light. In addition, the transcript levels of all investigated PEP-dependent genes were clearly reduced in the ptac14-1 mutants, while the accumulation of nucleus-encoded phage-type RNA polymerase-dependent transcripts was increased, indicating an important role of pTAC14 in maintaining PEP activity. pTAC14 was found to interact with pTAC12/ HEMERA, another component of TACs that is involved in phytochrome signaling. The data suggest that pTAC14 is essential for proper chloroplast development, most likely by affecting PEP activity and regulating PEP-dependent plastid gene transcription in Arabidopsis together with pTAC12.

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Section: Discussionmentioning

confidence: 88%

Section: Chloroplast Development Is Defective In Ptac14 Mutantsmentioning

confidence: 99%

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

et al. 2011

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“…Genes involved with removing radical oxygen species such as Iron Superoxide Dismutase1 (Myouga et al, 2008) and Catalase1 (Yoshida et al, 2003) showed declines in transcript and protein levels. An exception is GPX5 (a glutathione peroxidase), whose transcript levels increased by approximately 30% by 24 h of deprivation and whose protein abundance increased approximately 20%.…”

Section: Changes In the Levels Of Transcripts And Proteins Of Photosymentioning

confidence: 99%

The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii

Juergens

Deshpande²,

Lucker

et al. 2014

Plant Physiology

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The accumulation of carbon storage compounds by many unicellular algae after nutrient deprivation occurs despite declines in their photosynthetic apparatus. To understand the regulation and roles of photosynthesis during this potentially bioenergetically valuable process, we analyzed photosynthetic structure and function after nitrogen deprivation in the model alga Chlamydomonas reinhardtii. Transcriptomic, proteomic, metabolite, and lipid profiling and microscopic time course data were combined with multiple measures of photosynthetic function. Levels of transcripts and proteins of photosystems I and II and most antenna genes fell with differing trajectories; thylakoid membrane lipid levels decreased, while their proportions remained similar and thylakoid membrane organization appeared to be preserved. Cellular chlorophyll (Chl) content decreased more than 2-fold within 24 h, and we conclude from transcript protein and 13 C labeling rates that Chl synthesis was down-regulated both pre-and posttranslationally and that Chl levels fell because of a rapid cessation in synthesis and dilution by cellular growth rather than because of degradation. Photosynthetically driven oxygen production and the efficiency of photosystem II as well as P700 + reduction and electrochromic shift kinetics all decreased over the time course, without evidence of substantial energy overflow. The results also indicate that linear electron flow fell approximately 15% more than cyclic flow over the first 24 h. Comparing Calvin-Benson cycle transcript and enzyme levels with changes in photosynthetic 13 CO 2 incorporation rates also pointed to a coordinated multilevel down-regulation of photosynthetic fluxes during starch synthesis before the induction of high triacylglycerol accumulation rates.

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“…The plastid localization of FSD2 and FSD3 was confirmed using GFP fusions. FSD2 and FSD3 play key roles in early chloroplast development (Myouga et al, 2008). The 3 isoforms of Cu-ZnSOD in Arabidopsis have different subcellular locations.…”

Section: Introductionmentioning

confidence: 99%

The role of Mn-SOD and Fe-SOD genes in the response to low temperature in chs mutants of Arabidopsis

Gharari¹,

Nejad²,

Band³

et al. 2014

Turk J Bot

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To determine whether the expression of iron superoxide dismutase (Fe-SOD) and manganese superoxide dismutase (Mn-SOD) increase superoxide-scavenging capacity, and thereby improve the survival rate of chilling sensitive (chs) mutants of Arabidopsis, 4 chs mutant (chs1-1, chs1-2, chs2-1, and chs2-2) and wild-type plants were grown under low (chilling, 13 °C; cold, 4 °C) and normal growth (23 °C) temperatures. Photosynthetic parameters were investigated following treatment with chilling, cold, and normal growth conditions. Chlorophyll content and maximum quantum efficiency of PSII primary photochemistry (Fv/Fm) were reduced in plants grown at chilling stress. The degree of chilling sensitivity of chs1 mutant plants was significantly greater than that of wild-type and chs2 mutants, as measured by chlorophyll fluorescence value and chlorophyll content. MSD1 was expressed during chilling stress in chs mutant and WT plants, while expression of FSD2 and FSD3 SODs in chs mutants was not detected during any of the treatments. Our results suggest that MSD1 expression in chs1-chilled plants responds to chilling, and that the lack of expression of FSD2 and FSD3 genes in chs mutants grown at chilling temperature supports the hypothesis that chloroplasts might be damaged, due to the chs mutation, when they are chilled.

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A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development inArabidopsis

Cited by 279 publications

References 76 publications

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

The Regulation of Photosynthetic Structure and Function during Nitrogen Deprivation in Chlamydomonas reinhardtii

The role of Mn-SOD and Fe-SOD genes in the response to low temperature in chs mutants of Arabidopsis

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