A Henry's law method for generating bulk nanobubbles

Ferraro, Gianluca; Jadhav, Ananda J.; Barigou, Mostafa

doi:10.1039/d0nr03332d

Cited by 45 publications

(48 citation statements)

References 71 publications

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“…Despite such overwhelming applications, the fundamental understanding of nanobubble generation [9] and a unique characterization technique to distinguish nanobubbles from the particle and nanodroplet [10] are still under infancy. Broadly, nanobubble generation methods include acoustic cavitation [11] , porous membrane [12] , repeated compression and decompression [13] , [14] , electrolysis [15] , external electric field [16] and alternating magnetic field [17] . Several characterization techniques have been employed to detect nanobubbles.…”

Section: Introductionmentioning

confidence: 99%

Does salting-out effect nucleate nanobubbles in water: Spontaneous nucleation?

Agarwal

Trivedi

Nirmalkar

2022

Ultrasonics Sonochemistry

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Highlights Nanobubble density during salting-out effect increases with salting-out parameter. An ultrasound field with a high amplitude destroys the nanobubbles in salt solution. R.I. of nanobubbles in NaCl and AlCl 3 were estimated to be 1.012 and 1.302, respectively. The NBs during salting-out effect are observed to be stable only for up to three days.

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Section: Introductionmentioning

confidence: 99%

Does salting-out effect nucleate nanobubbles in water: Spontaneous nucleation?

Agarwal

Trivedi

Nirmalkar

2022

Ultrasonics Sonochemistry

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“…For the analysis of N-NBs in DMEM, a nanoparticle tracking analysis (NTA) method was used with a NTA instrument (NanoSight LM10-HSBFT14; Malvern, UK), which is widely used in the NB research eld 20,21 . It is a NB visualization technique that provides size, count and concentration measurements.…”

Section: Measurements Of Concentration and Size Of N-nbs In Dmemmentioning

confidence: 99%

Proliferative Effects of Nitrogen Nanobubbles on Fibroblasts

Heo¹,

Park²,

Lee³

et al. 2021

Preprint

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In recent years, the potential of nanobubbles (NBs) for biological activation has been actively investigated. In this study, we investigated the proliferative effects of nitrogen nanobubbles (N-NBs) on fibroblast cells using cell assays with image analysis and flow cytometry. A high concentration of N-NBs (more than 4 × 108 NBs/mL) was generated in Dulbecco’s modified Eagle’s medium (DMEM) using a gas-liquid mixing method. In image analysis, the cells were counted and compared, which showed an 11% improvement in proliferation in the culture medium with N-NBs. In flow cytometry, the decrease in the fluorescence intensity was analyzed, which revealed a 1.5% improvement in proliferation in the culture medium with N-NBs. This study represents the first successful attempt of directly generating quantified NBs in a culture medium for cell culture. The findings suggest that the N-NBs in the culture medium can facilitate cell proliferation.

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“…9 This prevents nanobubbles from rising to the free surface where the change in pressure of the atmosphere can cause a shi in the equilibrium between the pressure on the inside of the bubble and the pressure on the outside of the bubble, thereby causing the bubble to become unstable and burst. 9,11,14,15 Other explanations involve the physical chemistry of the nanobubbles. The electrically charged interface of the bubble creates strong repulsive forces between bubbles, which prevent bubble coalescence, bubble bursting, and Ostwald ripening.…”

Section: Introductionmentioning

confidence: 99%