A hemolysis–hemagglutination assay for characterizing constitutive innate humoral immunity in wild and domestic birds

Matson, Kevin D.; Ricklefs, Robert E.; Klasing, Kirk C.

doi:10.1016/j.dci.2004.07.006

Cited by 380 publications

(548 citation statements)

References 36 publications

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“…However, both measures were comparable to those of a population from St. Louis, MO, USA (Matson et al 2005). …”

Section: Constitutive Defensesmentioning

confidence: 76%

“…Seventy minutes later, this procedure was repeated. Photographs were taken at these time points in particular to enable to score both agglutination and lysis in the same plasma sample (Matson et al 2005). Maximum agglutination was scored as the concentration of plasma in which the blood cell pellet remained completely intact after 20 min of tilting.…”

Section: Natural Antibody Levels and Lysis Activitymentioning

confidence: 99%

“…We used a rabbit red blood cell (RRBC) agglutination/ lysis assay to characterize natural antibody (NAb) levels (predominantly IgM) and complement/NAb-mediated lysis capacity of plasma (Matson et al 2005). In February-April 2003, we captured wild birds and took 50 ll blood samples from each animal; plasma was then stored at À20°C until assay (January 2004).…”

Section: Natural Antibody Levels and Lysis Activitymentioning

confidence: 99%

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Investment in immune defense is linked to pace of life in house sparrows

2006

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The evidence for a relationship between life history and immune defense is equivocal, although the basic premise is intuitively appealing: animals that live short lives and reproduce early and rapidly should not waste resources on defenses they might never use. One possible reason for a lack of strong support for this hypothesis could be the inherent complexity of the vertebrate immune system. Indeed, different components of the vertebrate immune system vary in their relative costs and benefits, and therefore only some defenses may complement variation in species' life history. To address this hypothesis, we compared multiple types of immune activity between two populations of house sparrows (Passer domesticus) with distinct life histories, one from Colon, Panama, which lay small clutches over an extended breeding season (i.e., slow-living) and the other from Princeton, New Jersey, which lay larger clutches in a smaller window of time (i.e., fast-living). We expected (a) that more costly types of immune defenses would be stronger in the slow-living sparrows and (2) that the slow-living sparrows would show a greater increase in whole-body energy expenditure after immune challenge compared to their fast-living counterparts. We found that secondary antibody response to a novel antigen was more rapid and energetic investment in immune activity was greater in slow-living sparrows. However, cellmediated immune activity was more robust in fast-living sparrows, and other measures of defense were not different between populations. These results provide partial support for a relationship between life history and immune defense in this species, but they also indicate that this relationship is not clear-cut. Further study is necessary to identify the influence of other factors, particular pathogen environment during development, on the architecture of the immune system of wild animals.

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“…However, both measures were comparable to those of a population from St. Louis, MO, USA (Matson et al 2005). …”

Section: Constitutive Defensesmentioning

confidence: 76%

Section: Natural Antibody Levels and Lysis Activitymentioning

confidence: 99%

Section: Natural Antibody Levels and Lysis Activitymentioning

confidence: 99%

See 1 more Smart Citation

Investment in immune defense is linked to pace of life in house sparrows

2006

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“…We also measured the activity of natural antibodies that circulate in normal individuals in the absence of exogenous antigenic stimulation as a measure of constitutive innate immune defences [14]. We followed the protocol by Matson et al [12] scaled to our sample sizes of plasma (between 15 and 25 ml). Plasma samples were serially diluted twofold with 0.01 M PBS (Sigma P3813) and incubated at 378C for 90 min with a 1 per cent rabbit blood cell suspension (Colorado Serum, CO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…We used two common assays of immune function [5,11,12] that have been related to offspring survival and disease resistance (e.g. [13]).…”

Section: Methodsmentioning

confidence: 99%

A trade-off between embryonic development rate and immune function of avian offspring is revealed by considering embryonic temperature

2011

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Long embryonic periods are assumed to reflect slower intrinsic development that are thought to trade off to allow enhanced physiological systems, such as immune function. Yet, the relatively rare studies of this trade-off in avian offspring have not found the expected trade-off. Theory and tests have not taken into account the strong extrinsic effects of temperature on embryonic periods of birds. Here, we show that length of the embryonic period did not explain variation in two measures of immune function when temperature was ignored, based on studies of 34 Passerine species in tropical Venezuela (23 species) and north temperate Arizona (11 species). Variation in immune function was explained when embryonic periods were corrected for average embryonic temperature, in order to better estimate intrinsic rates of development. Immune function of offspring trades off with intrinsic rates of embryonic development once the extrinsic effects of embryonic temperatures are taken into account.

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Ecoimmunology in the field: Measuring multiple dimensions of immune function with minimally invasive, field‐adapted techniques

Blackwell

García

2022

American J Hum Biol

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Immune function is multifaceted and characterizations based on single biomarkers may be uninformative or misleading, particularly when considered across ecological contexts. However, measuring the many facets of immunity in the field can be challenging, since many measures cannot be obtained on-site, necessitating sample preservation and transport. Here we assess state-of-the-art methods for measuring immunity, focusing on measures that require a minimal blood sample obtained from a finger prick, which can be: (1) dried on filter paper, (2) frozen in liquid nitrogen, or (3) stabilized with chemical reagents. Results:We review immune measures that can be obtained from point-of-care devices or from immunoassays of dried blood spots (DBSs), field methods for flow cytometry, the use of RNA or DNA sequencing and quantification, and the application of immune activation assays under field conditions. Conclusions: Stable protein products, such as immunoglobulins and C-

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A hemolysis–hemagglutination assay for characterizing constitutive innate humoral immunity in wild and domestic birds

Cited by 380 publications

References 36 publications

Investment in immune defense is linked to pace of life in house sparrows

Investment in immune defense is linked to pace of life in house sparrows

A trade-off between embryonic development rate and immune function of avian offspring is revealed by considering embryonic temperature

Ecoimmunology in the field: Measuring multiple dimensions of immune function with minimally invasive, field‐adapted techniques

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