A guide to the optogenetic regulation of endogenous molecules

Manoilov, K. Yu.; Verkhusha, Vladislav V.; Shcherbakova, Daria M.

doi:10.1038/s41592-021-01240-1

Cited by 24 publications

(12 citation statements)

References 94 publications

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“…Many light-modulated actuators have been described that do not rely on opsin proteins. While this Primer is focused on the opsin-based toolbox, the reader might find more information about non-opsin-based optogenetic tools in several excellent recent reviews 17,18 .…”

Section: [H2] Selecting the Correct Actuatormentioning

confidence: 99%

Optogenetics for light control of biological systems

Emiliani

Entcheva

Hedrich

et al. 2022

Nat Rev Methods Primers

172

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Optogenetic techniques have been developed to allow control over the activity of selected cells within a highly heterogeneous tissue, using a combination of genetic engineering and light. Optogenetics employs natural and engineered photoreceptors, mostly of microbial origin, to be genetically introduced into the cells of interest. As a result, cells that are naturally light-insensitive can be made photosensitive and addressable by illumination and precisely controllable in time and space. The selectivity of expression and subcellular targeting in the host is enabled by applying control elements such as promoters, enhancers, and specific targeting sequences to the employed photoreceptorencoding DNA. This powerful approach allows precise characterization and manipulation of cellular

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Section: [H2] Selecting the Correct Actuatormentioning

confidence: 99%

Optogenetics for light control of biological systems

Emiliani

Entcheva

Hedrich

et al. 2022

Nat Rev Methods Primers

172

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“…Phytochromes have the advantage to induce protein dimerization in response to red light, with far-red light reversing the reaction, therefore acting like an active ON-OFF toggle switch (Figure 5B). Each photoreceptor has distinct sensitivity and kinetics of dimerization; thus, they can be engineered to achieve various optogenetic properties in terms of speed of action, reversibility and responsiveness [77]. Like the rapamycin-inducible method, it is now possible to manipulate the levels of many PI species using various enzymatic modules at different intracellular membranes (see Table 1).…”

Section: Optogenetics: Light-induced Genetic Systems For Acute Manipu...mentioning

confidence: 99%

Experimental manipulation of phosphoinositide lipids: from cells to organisms

Doumane¹,

Caillaud²,

Jaillais³

2022

Trends in Cell Biology

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“…In terms of spatial control, the light-controlled approach has become a topic of growing interest during the past couple of years due to its unprecedented spatiotemporal precision. [9][10][11] The majority of such methods rely on the installation of light-sensitive moieties on biological macromolecules for the optogenetic [12][13][14] or photochemical [15][16][17][18] regulation of gene and protein activity. Among these strategies, photocaging of nucleic acids allows to modulate the expression or localization of the target gene in a sitespecific manner.…”

Section: Introductionmentioning

confidence: 99%

A Remotely Controlled Nanosystem for Spatiotemporally Specific Gene Regulation and Combinational Tumor Therapy

Chai

Chen

et al. 2023

Angew Chem Int Ed

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The dearth of technologies that allow gene modulation and therapy with high spatiotemporal precision remains a bottleneck in biomedical research and applications. Here we present a near-infrared (NIR) light-controlled nanosystem that allows spatiotemporally controlled regulation of gene expression and thus combinational tumor therapy. The nanosystem is built by engineering of an enzyme-activatable antisense oligonucleotide and further combination with an upconversion nanoparticle-based photodynamic system and a mitochondria localization signal. The system relies on photodynamic effect-induced translocation of a DNA repair enzyme from nucleus into mitochondria, which enables spatially selective gene regulation via enzymatic reactions. We demonstrate that the NIR light-induced mitochondrial photodamage and gene regulation enable enhanced antitumor effect. Our approach may enable the specific gene regulation and tumor treatment with high precision both spatially and temporally.

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A guide to the optogenetic regulation of endogenous molecules

Cited by 24 publications

References 94 publications

Optogenetics for light control of biological systems

Optogenetics for light control of biological systems

Experimental manipulation of phosphoinositide lipids: from cells to organisms

A Remotely Controlled Nanosystem for Spatiotemporally Specific Gene Regulation and Combinational Tumor Therapy

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