A Group‐Specific PCR Assay for the Detection of <i>Helicobacteraceae</i> in Human Gut

Bohr, Ulrich R. M.; Primus, Anett; Zagoura, Alexandra; Glasbrenner, B.; Wex, Thomas; Malfertheiner, Peter

doi:10.1046/j.1523-5378.2002.00113.x

Cited by 43 publications

(38 citation statements)

References 35 publications

(47 reference statements)

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“…Basically, two types of PCR assays were developed: speciesspecific PCR assays that allow the identification of single Helicobacter species (27,30) and Helicobacter group-specific PCR assays which can detect several Helicobacter species in a single assay (2,25,27). In samples that are positive in a group-specific PCR a further step is required to identify the Helicobacter species.…”

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“…In samples that are positive in a group-specific PCR a further step is required to identify the Helicobacter species. DNA sequencing and restriction fragment length polymorphism (RFLP) analysis are commonly used for this purpose (2,27), although denaturing gradient gel electrophoresis has also been successfully used (25). Here, we used genusspecific PCR and consecutive DNA sequencing combined with RFLP analysis to evaluate the prevalence of Helicobacter infections and their spread in the SPF facility of our central animal laboratory.…”

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Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

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Infections with enterohepatic Helicobacter species (EHS) can change the results of animal experiments.However, there is little information about the prevalence of EHS in noncommercial animal facilities. The aim of this study was to investigate the prevalence and the spread of EHS in specific-pathogen-free (SPF) mice. Fecal samples of 40 mouse lines were analyzed for members of the family Helicobacteraceae using a group-specific PCR targeting the 16S rRNA gene. Additional experiments were carried out to evaluate the spread of EHS among mice harbored in different caging systems. Helicobacter species were detected in 87.5% of the mouse lines tested. Five different Helicobacter species were identified: H. ganmani, H. hepaticus, H. typhlonicus, and the putative Helicobacter species represented by the isolates hamster B and MIT 98-5357. Helicobacter infection did not spread between animals in neighboring cages when individually ventilated cages were used; in contrast, when the mice were reared in open-air cages, EHS were found to spread from cage to cage. However, the spread was prevented by adding polycarbonate filter tops to the cages. When Helicobacter-negative and infected mice shared the same cage, transmission of the infection occurred in 100% within 2 weeks. Furthermore, we found that mice from commercial breeding facilities may carry undetected Helicobacter infections. Taken together, we show that infection with EHS may frequently occur and spread easily in mice reared under SPF conditions despite extensive safety precautions. Moreover, there is a high prevalence of rather uncommon Helicobacter species that may be a consequence of the current routine procedures used for health screening of SPF mice.

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Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

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“…A number of research groups have associated this organism with vibrionic hepatitis and enteritis in poultry and with gastroenteritis, diarrhea, and liver and gall bladder disease in human patients (8,9,18,28). It has been suggested that H. pullorum also may play a role in Crohn's disease (1,4).…”

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Prevalence ofHelicobacter pullorumamong Patients with Gastrointestinal Disease and Clinically Healthy Persons

et al. 2005

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Feces from 531 patients with gastroenteritis and from 100 clinically healthy individuals were tested for Helicobacter pullorum by use of PCR. Samples positive by PCR were qualified for isolation. H. pullorum DNA was demonstrated to be present in feces from 4.3% of patients with gastrointestinal disease but also in feces from 4.0% of clinically healthy persons. One strain was isolated from one patient with gastrointestinal disease.

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“…Biopsy specimens from the terminal ileum and the colon of each patient were analyzed by a group-specific PCR assay with primers C97 (5Ј-GCTATGACGGGTATCC-3Ј) and H2 (5Ј-TCGCAATGAGTATTCCTCTT-3Ј) as previously described (2,3). In order to identify the Helicobacter species, amplification products were sequenced as described before (3).…”

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Identification of Enterohepatic Helicobacter Species in Patients Suffering from Inflammatory Bowel Disease

et al. 2004

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A Group‐Specific PCR Assay for the Detection of Helicobacteraceae in Human Gut

Abstract: The novel PCR assay described here is an important new tool in rapid and sensitive assessment for the presence of Helicobacteraceae in human gut.

Cited by 43 publications

References 35 publications

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Prevalence ofHelicobacter pullorumamong Patients with Gastrointestinal Disease and Clinically Healthy Persons

Identification of Enterohepatic Helicobacter Species in Patients Suffering from Inflammatory Bowel Disease

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