SOME PHYSIOLOGICAL CONCEPTS, such physiology of filtration and absorption in the different nephron segments, are so detailed that they can be a challenge to be memorized. This article describes an exercise that solidifies learning as students manipulate, using paper models, "transporters" and "electrolytes" in the basolateral and luminal membranes of "nephron cells." Most important is the opportunity for the students to apply and test their own knowledge related to changes that occur in physiological processes, such as excess of salt or water intake or blood volume alterations, or pathology, such as syndrome of inappropriate antidiuretic hormone secretion, among many other applications.
Description of the ManipulativeThis study was applied to first-year medical students at the end of renal physiology lectures at Virginia Tech Carilion Medical School. The protocol was granted exempt status by the Virginia Tech Institutional Review Board (IRB no. 14-039). Students working in self-assigned groups of four or five were given packets containing all necessary materials. Figures 1-3 show examples of one cell, some transporters, and plasma used in this initial study. Complete material is available upon request. Each packet included paper diagrams (8.5 ϫ 11 in.) of the major cell types of the nephron (see Fig. 1 for the early proximal tubule). Small pieces of color-coded cardstock were constructed to represent the major transporters and ion channels present within the various epithelial cells of the nephron (see Fig. 2 for examples of some transporters). Strips of grid paper were used to represent several prominent ions, which were color coded to match the transporters. Ions used in the demonstration included Na ϩ , Cl Ϫ , K ϩ , HCO 3Ϫ , water, amino acids, and glucose, where each box of the grid paper represented 10% of the filtered load of the respective ions (Fig. 3).One important benefit of this teaching methodology is the ability to adapt the manipulative to best fit the instructor's learning objectives. Lecturers may add or subtract pieces from the model to best suit their own classroom needs. In the work described here, we did not cover the transport of Mg 2ϩ , Ca 2ϩ , urea, organic anions, organic cations, carbohydrates, urate, and ammonia, but they can easily be included using the manipulative.
Use of the ManipulativePart 1: basics of the nephron manipulative. Initially, groups were asked to place the cell types of the nephron in sequential order, beginning with the early proximal convoluted tubule and ending with cells of the collecting duct. Groups were then challenged to place the pieces representing the various transporters and ion channels present in the tubular epithelium in their correct locations. Correct placement required students to know not only the appropriate region of the nephron (e.g., the early proximal tubule, late proximal tubule, early distal tubule, and collecting duct) but also the correct side of the epithelial cell (i.e., basolateral or lumen interface). This setup allowed students to asses...