A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis

Bai, Yang; Shen, Yue; Zhang, Zhiqiang; Jia, Qianru; Xu, Mengyuan; Zhang, Ting; Hailing, Fang; Yu, Xu; Li, Li; Liu, Dongmei; Xiwu, Qi; Chen, Zhide; Wu, Shuang; Zhang, Qun; Chengyuan, Liang

doi:10.3390/ijms22020785

Cited by 5 publications

(7 citation statements)

References 88 publications

(74 reference statements)

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Section: Crispr/cas9 and Lipid Metabolic Engineeringmentioning

confidence: 99%

“…As a result of the deletion of Arabidopsis GPAT1 , SFAs content decreased and MUFAs content increased ( Bai et al, 2021 ). Plant height and cell length increased, but oil content decreased in gpat1 mutants ( Bai et al, 2021 ). In rapeseed, Bnlpat2 and Bnlpat5 single mutants increased the content of 18:0 and 20:0, and decreased the content of 18:1, 18:2, and 18:3 ( Zhang et al, 2019b ).…”

Section: Crispr/cas9 and Lipid Metabolic Engineeringmentioning

confidence: 99%

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Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis

Park

Kim²

2022

Front. Plant Sci.

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Triacylglycerol (TAG), which is a neutral lipid, has a structure in which three molecules of fatty acid (FA) are ester-bonded to one molecule of glycerol. TAG is important energy source for seed germination and seedling development in plants. Depending on the FA composition of the TAG, it is used as an edible oil or industrial material for cosmetics, soap, and lubricant. As the demand for plant oil is rising worldwide, either the type of FA must be changed or the total oil content of various plants must be increased. In this review, we discuss the regulation of FA metabolism by Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9, a recent genome-editing technology applicable to various plants. The development of plants with higher levels of oleic acid or lower levels of very long-chain fatty acids (VLCFAs) in seeds are discussed. In addition, the current status of research on acyltransferases, phospholipases, TAG lipases, and TAG synthesis in vegetative tissues is described. Finally, strategies for the application of CRISPR/Cas9 in lipid metabolism studies are mentioned.

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Section: Crispr/cas9 and Lipid Metabolic Engineeringmentioning

confidence: 99%

Section: Crispr/cas9 and Lipid Metabolic Engineeringmentioning

confidence: 99%

Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis

Park

Kim²

2022

Front. Plant Sci.

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“…At least 23 and two transcripts were up- and downregulated upon RAP2.12 overexpression, respectively, including AOX1b (5.1-fold induced). In addition, seeds from the prt6 mutant presented alterations in the mitochondrial transcripts [ 78 ] involved in central carbon and lipid metabolisms, such as QUA-QUINE STARCH ( QQS ) [ 111 ], GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 1 ( GPAT1 ) [ 112 ], and DUF581 CYCLIN-DEPENDENT KINASE ( AT1G74940 ), among which the latter was shown to interact with SNRK1 [ 113 ]. Moreover, AOX1a and NDB3 were downregulated in seeds from the prt6 mutant [ 78 ].…”

Section: Mitochondrial Retrograde Signaling and Crosstalk With Chloro...mentioning

confidence: 99%

Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses

Barreto

Koltun²,

Nonato³

et al. 2022

IJMS

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The interaction of mitochondria with cellular components evolved differently in plants and mammals; in plants, the organelle contains proteins such as ALTERNATIVE OXIDASES (AOXs), which, in conjunction with internal and external ALTERNATIVE NAD(P)H DEHYDROGENASES, allow canonical oxidative phosphorylation (OXPHOS) to be bypassed. Plant mitochondria also contain UNCOUPLING PROTEINS (UCPs) that bypass OXPHOS. Recent work revealed that OXPHOS bypass performed by AOXs and UCPs is linked with new mechanisms of mitochondrial retrograde signaling. AOX is functionally associated with the NO APICAL MERISTEM transcription factors, which mediate mitochondrial retrograde signaling, while UCP1 can regulate the plant oxygen-sensing mechanism via the PRT6 N-Degron. Here, we discuss the crosstalk or the independent action of AOXs and UCPs on mitochondrial retrograde signaling associated with abiotic stress responses. We also discuss how mitochondrial function and retrograde signaling mechanisms affect chloroplast function. Additionally, we discuss how mitochondrial inner membrane transporters can mediate mitochondrial communication with other organelles. Lastly, we review how mitochondrial metabolism can be used to improve crop resilience to environmental stresses. In this respect, we particularly focus on the contribution of Brazilian research groups to advances in the topic of mitochondrial metabolism and signaling.

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“…It has been noted that genetic dissection and the use of specific plant-type traits, including plant height, can improve the efficiency of soybean high-yield breeding ( Liu et al, 2020 ). Plant height, as a complex quantitative trait, is governed by abundant genes and a variety of hormones, such as gibberellin, auxin, and cytokinin ( Wang et al, 2017 , 2021 ; Zhang et al, 2017 ; Dong et al, 2019 ; Liao et al, 2019 ; Castorina and Consonni, 2020 ; Bai et al, 2021 ). During the last century’s “Green Revolution,” the Rht1 ( Reduced height 1 ) gene of wheat ( Rockwell and Hongay, 2019 ), the Sd1 ( Semi-dwarf 1 ) gene of rice ( Ren et al, 2019 ), and the D8 ( Dwarf-8 ) gene of maize ( Huang et al, 2019 ) all regulated plant height by adjusting gibberellic acid (GA) contents.…”

Section: Introductionmentioning

confidence: 99%

An APETALA2/ethylene responsive factor transcription factor GmCRF4a regulates plant height and auxin biosynthesis in soybean

Wang²,

Kong³

et al. 2022

Front. Plant Sci.

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Plant height is one of the key agronomic traits affecting soybean yield. The cytokinin response factors (CRFs), as a branch of the APETALA2/ethylene responsive factor (AP2/ERF) super gene family, have been reported to play important roles in regulating plant growth and development. However, their functions in soybean remain unknown. This study characterized a soybean CRF gene named GmCRF4a by comparing the performance of the homozygous Gmcrf4a-1 mutant, GmCRF4a overexpression (OX) and co-silencing (CS) lines. Phenotypic analysis showed that overexpression of GmCRF4a resulted in taller hypocotyls and epicotyls, more main stem nodes, and higher plant height. While down-regulation of GmCRF4a conferred shorter hypocotyls and epicotyls, as well as a reduction in plant height. The histological analysis results demonstrated that GmCRF4a promotes epicotyl elongation primarily by increasing cell length. Furthermore, GmCRF4a is required for the expression of GmYUCs genes to elevate endogenous auxin levels, which may subsequently enhance stem elongation. Taken together, these observations describe a novel regulatory mechanism in soybean, and provide the basis for elucidating the function of GmCRF4a in auxin biosynthesis pathway and plant heigh regulation in plants.

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A GPAT1 Mutation in Arabidopsis Enhances Plant Height but Impairs Seed Oil Biosynthesis

Cited by 5 publications

References 88 publications

Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis

Applications and prospects of genome editing in plant fatty acid and triacylglycerol biosynthesis

Metabolism and Signaling of Plant Mitochondria in Adaptation to Environmental Stresses

An APETALA2/ethylene responsive factor transcription factor GmCRF4a regulates plant height and auxin biosynthesis in soybean

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