A glycine-rich protein MoGrp1 functions as a novel splicing factor to regulate fungal virulence and growth in Magnaporthe oryzae

Gao, Xusheng; Yin, Changfa; Liu, Xinsen; Peng, Junbo; Deng, Chao; He, Dan; Shi, Wei; Zhao, Wensheng; Yang, Jun; Peng, You-Liang

doi:10.1186/s42483-018-0007-1

Cited by 24 publications

(28 citation statements)

References 70 publications

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“…To test whether this measurement works for secreted proteins, the fluorescence intensity of the P131 strain expressing control GFP alone was measured and the rate of secretion was calculated as 0.08, indicating that unfused GFP was not secreted into the liquid CM. Moreover, Slp1-GFP was secreted in the P131 strain at a rate of 0.36, while the nuclei-localized protein MoGRP1-GFP was secreted at a rate of 0.06, supporting the observation that Slp1-GFP but not MoGRP1-GFP was secreted into the liquid CM in the P131 strain [27] (Supplemental Fig. 2B).…”

Section: Resultssupporting

confidence: 72%

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Liu

Huang

et al. 2020

Preprint

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Plant fungal pathogens secrete numerous proteins into the apoplast at the plant–fungus contact sites to facilitate colonization. Only a few secreted proteins were functionally characterized in Magnaporthe oryzae, the fungal pathogen causing rice blast disease worldwide. ALG3 is an α-1, 3-mannosyltransferase function in N-glycan synthesis for secreted N-glycosylated proteins, and the Δalg3 mutants show strong defects in cell wall integrity and fungal virulence, indicating a potential effect on the secretion of multiple proteins. In this study, we compared the secretome of wild type and Δalg3 mutants, and identified 51 proteins that require ALG3 for proper secretion. These are predicted to be involved in metabolic processes, interspecies interactions, cell wall organization, and response to chemicals. The tested secreted proteins localized at the apoplast region surrounding the fungal infection hyphae. Moreover, the N-glycosylation of candidate proteins was significantly changed in the Δalg3 mutant, leading to the reduction of protein secretion and abnormal protein localization. Furthermore, we tested the function of two genes, one is a previously reported M. oryzae gene Invertase 1 (INV1) encoding a secreted invertase, and the other one is a gene encoding an Acid mammalian chinitase (AMCase). The fungal virulence was significantly reduced and the cell wall integrity was altered in the Δinv1 and Δamcase mutant strains. Elucidation of the comparative secretome of M. oryzae improves our understanding of the proteins that require ALG3 for secretion, and of their function in fungal virulence and cell wall integrity.

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Section: Resultssupporting

confidence: 72%

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Liu

Huang

et al. 2020

Preprint

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“…The important roles of a few splicing factors in the growth, development, and virulence of rice pathogens have been identified in recent genetic and transcriptomic studies. For example, the auxiliary splicing factor gene MoGRP1 (encoding a glycine-rich protein) modulates growth, development, virulence, and stress responses in rice blast fungus [ 169 ]. MoGRP1 interacts with spliceosome components and plays a crucial role in modulating hyphal growth and conidiation by regulating the normal splicing of the corresponding genes, such as MST7 and MoRAD6 .…”

Section: Biotic Stressmentioning

confidence: 99%

“…MoGRP1 interacts with spliceosome components and plays a crucial role in modulating hyphal growth and conidiation by regulating the normal splicing of the corresponding genes, such as MST7 and MoRAD6 . Furthermore, besides their impaired mycelial growth, conidiation, and virulence, the Mogrp1 mutant fungi are unable to cause lesions on rice leaves [ 169 ]. Similarly, the silencing of SR gene NlSRp54 greatly decreases the survival of virulent BPH on resistant Mudgo rice, implicating an essential role of AS in the BPH-rice interaction and possibly in the rice resistance breakdown [ 158 ].…”

Section: Biotic Stressmentioning

confidence: 99%

Stress-Induced Changes in Alternative Splicing Landscape in Rice: Functional Significance of Splice Isoforms in Stress Tolerance

Ganie

Reddy

2021

Biology

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Improvements in yield and quality of rice are crucial for global food security. However, global rice production is substantially hindered by various biotic and abiotic stresses. Making further improvements in rice yield is a major challenge to the rice research community, which can be accomplished through developing abiotic stress-resilient rice varieties and engineering durable agrochemical-independent pathogen resistance in high-yielding elite rice varieties. This, in turn, needs increased understanding of the mechanisms by which stresses affect rice growth and development. Alternative splicing (AS), a post-transcriptional gene regulatory mechanism, allows rapid changes in the transcriptome and can generate novel regulatory mechanisms to confer plasticity to plant growth and development. Mounting evidence indicates that AS has a prominent role in regulating rice growth and development under stress conditions. Several regulatory and structural genes and splicing factors of rice undergo different types of stress-induced AS events, and the functional significance of some of them in stress tolerance has been defined. Both rice and its pathogens use this complex regulatory mechanism to devise strategies against each other. This review covers the current understanding and evidence for the involvement of AS in biotic and abiotic stress-responsive genes, and its relevance to rice growth and development. Furthermore, we discuss implications of AS for the virulence of different rice pathogens and highlight the areas of further research and potential future avenues to develop climate-smart and disease-resistant rice varieties.

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“…Another study reported that Srk1 participates in the developmental stages of vegetative growth, sexual reproduction, and plant infection and is also required for pre‐mRNA AS and gene expression in F. graminearum (Wang et al ., ). MoGrp1, a novel splicing factor with poly(U)‐binding activity, plays a critical role in regulating fungal virulence, development, and stress responses (Gao et al ., ). These studies illustrate that posttranscriptional modification is required for fungal pathogenicity and development.…”

Section: Discussionmentioning

confidence: 97%

Arginine methylation is required for remodelling pre‐mRNA splicing and induction of autophagy in rice blast fungus

Li-ye

et al. 2019

New Phytologist

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Summary Protein arginine methyltransferases (PRMTs) regulate many physiological processes, including autophagy. However, the direct roles of the various PRMTs during autophagosome formation remain unclear. Here, we characterised the function of MoHMT1 in the rice blast fungus, Magnaporthe oryzae. Knockout of MoHMT1 results in inhibited growth and a decreased ability to cause disease lesions on rice seedlings. MoHMT1 catalyses the di‐methylation of arginine 247, 251, 261 and 271 residues of MoSNP1, a U1 small nuclear ribonucleoprotein (snRNP) component, likely in a manner dependent on direct interaction. RNA‐seq analysis revealed that alternative splicing of pre‐mRNAs of 558 genes, including the autophagy‐related (ATG) gene MoATG4, was altered in MoHMT1 deletion mutants, compared with wild‐type strains under normal growth conditions. During light exposure or nitrogen starvation, MoHMT1 localises to autophagosomes and MoHMT1 mutants display defects in autophagy induction. Under nitrogen starvation, six additional MoATG genes were identified with retained introns in their mRNA transcripts, corresponding with a significant reduction in transcripts of intron‐spliced isoforms in the MoHMT1 mutant strain. Our study shows that arginine methylation plays an essential role in accurate pre‐mRNA splicing necessary for a range of developmental processes, including autophagosome formation.

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A glycine-rich protein MoGrp1 functions as a novel splicing factor to regulate fungal virulence and growth in Magnaporthe oryzae

Cited by 24 publications

References 70 publications

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Comparative secretome ofMagnaporthe oryzaeidentified proteins involved in virulence and cell wall integrity

Stress-Induced Changes in Alternative Splicing Landscape in Rice: Functional Significance of Splice Isoforms in Stress Tolerance

Arginine methylation is required for remodelling pre‐mRNA splicing and induction of autophagy in rice blast fungus

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