A genetic linkage map of restriction fragment length polymorphism loci for <i>Brassica rapa</i> (syn. <i>campestris</i>)

Chyi, Y.-S.; Hoenecke, M. E.; Sernyk, J. L.

doi:10.1139/g92-115

Cited by 106 publications

(24 citation statements)

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“…Several flowering-time genes from Arabidopsis (2 loci/2 probes), B. rapa (7 loci/6 probes), and BrMyrosinase, BrDFRI, BrGST, BrSAM, and BrSLP (10 loci/5 probes) functional genes were mapped on this linkage map. The detection of 1.31 loci per RFLP probe (520/396) closely matches that reported (1.27 and 1.34 from 220 and 269 probes, respectively) for previous B. rapa maps (Song et al 1991;Chyi et al 1992). In contrast, Udall et al (2005) observed a higher level of polymorphism with codominant and dominant segregation in the amphidiploid B. napus, where there are twice as many potential loci.…”

Section: Discussionsupporting

confidence: 88%

“…The high degree of neutral DNA polymorphisms of most Brassica species (Figdore et al 1988) has facilitated the development of molecular linkage maps, with at least 15 described to date for B. oleracea (Slocum et al 1990;Kianian and Quiros 1992;Lan et al 2000), B. rapa (Song et al 1991;Chyi et al 1992;Teutenico and Osborn 1994), B. nigra (Lagercrantz and Lydiate 1996), B. Juncea (Cheung et al 1997;Pradhan et al 2003), and B. napus (Landry et al 1991;Uzunova et al 1995). Where common sets of DNA markers and/or parental genotypes have been used, it has been possible to designate linkage groups according to a common nomenclature (Parkin et al 1995(Parkin et al , 2005Butruille et al 1999;Sebastian et al 2000).…”

Section: T He Genus Brassica Includes Oilseed Vegetablementioning

confidence: 99%

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A Sequence-Tagged Linkage Map ofBrassica rapa

et al. 2006

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A detailed genetic linkage map of Brassica rapa has been constructed containing 545 sequence-tagged loci covering 1287 cM, with an average mapping interval of 2.4 cM. The loci were identified using a combination of 520 RFLP and 25 PCR-based markers. RFLP probes were derived from 359 B. rapa EST clones and amplification products of 11 B. rapa and 26 Arabidopsis. Including 21 SSR markers provided anchors to previously published linkage maps for B. rapa and B. napus and is followed as the referenced mapping of R1-R10. The sequence-tagged markers allowed interpretation of the pattern of chromosome duplications within the B. rapa genome and comparison with Arabidopsis. A total of 62 EST markers showing a single RFLP band were mapped through 10 linkage groups, indicating that these can be valuable anchoring markers for chromosome-based genome sequencing of B. rapa. Other RFLP probes gave rise to 2-5 loci, inferring that B. rapa genome duplication is a general phenomenon through 10 chromosomes. The map includes five loci of FLC paralogues, which represent the previously reported BrFLC-1, -2, -3, and -5 and additionally identified BrFLC3 paralogues derived from local segmental duplication on R3.

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Section: Discussionsupporting

confidence: 88%

Section: T He Genus Brassica Includes Oilseed Vegetablementioning

confidence: 99%

A Sequence-Tagged Linkage Map ofBrassica rapa

et al. 2006

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“…The Brassica probes tested would be suitable for characterizing the addition and trisomic lines developed from the current material CHENG et al 1993CHENG et al , 1994aCHENG et al ,b, 1995a. Various Brassica probes have been used in the analysis of B. campestris-alboglabra addition lines or segregating F, populations, which has yielded a number of linkage maps for B. oleracea (SLOCUM et al 1990;HU and QUIROS 1991;LANDRY et al 1992;KIANIAN and QUIROS 1992b;FIGDORE et al 1993;KENNARD et al 1994;TEUTONICO and OSBORN 1994) and for B. campestris (SONG et al 1991;CHYI et al 1992;TEUTONICO and OSBORN 1994). The combination of RFLP markers with isozyme and morphological traits has been instrumental in constructing linkage maps for B. campestris and B. oleracea (HOSAKA et al 1990;QUIROS 1990, 1991a;KIANIAN and QUIROS 1992b).…”

Section: Discussionmentioning

confidence: 99%

“…Linkage maps, of variable saturation extents, have been developed for the A and C genomes of Brassica when applying restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and DNA fingerprinting (QUIROS et al 1987(QUIROS et al , 1994DELSENY et al 1990;HOSAKA et al 1990;SLOCUM et al 1990; HU and QUIROS 1991; MC- GRATH andQUIROS 1991a, 1992;SONG et al 1988SONG et al , 1991CHYI et al 1992; KIANIAN and QUIROS 1992a,b;LANDRY et al 1992;KENNARD et al 1994;POULSEN et al 1994). In addition to the linkage mapping of different molecular markers, these investigations gave us insight into the occurrence of gene duplications, chromosome structural changes and intergenomic gene introgression.…”

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confidence: 99%

Characterization of the A and C Genomes of Brassica Campestrisand B. Alboglabra

Heneen¹,

Chen²,

Cheng³

et al. 2004

Hereditas

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Meiosis was studied in pollen mother cells of two Brassica cumpestris (AA) and one B. alboglabru (CC) accessions, their resynthesized B. napus amphidiploids (AACC), and digenomic hybrids produced (AAC). The two parental species exhibited different chromatin condensation patterns at diakinesis, which enabled the differentiation between genomes and the detection of auto-and allosyndesis in the amphidiploids and digenomic hybrids. Corresponding chromatin condensation differences were manifested at mitotic prometaphase. The diploid accessions were characterized as to glucosinolate composition, restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme patterns. All 20 RFLP probes and 9 RAPD primers tested gave discriminatory bands for the A and C genomes. For isozymes, 14 out of 17 analysed loci had alleles that discriminated between these genomes. In addition to the already established locations of flower colour, erucic acid content, and leucine aminopeptidase enzyme genes in the C genome, other genome-specific isozyme and DNA-based markers amounted to 89 and 112 in the two B. campestris accessions and 135 in the B. alboglabra accession. Sinigrin synthesis is an additional character controlled by the C genome, The combination of different marker systems and cytological techniques for the characterization of the A and C genomes and their constituent chromosomes has obvious implications for plant breeding, assessment of gene introgression in nature, and evolutionary studies. W.K. Heneen, Department of Plant Breeding Research, The Swedish University of Agricultural Sciences, S-268 31 Svalov, SwedenThe genus Brassica comprises a large number of crops that serve as sources of oilseed, and as vegetables and fodder. In economic terms, the diploid species B. campestris L. (2n = 20, genome AA) and B. oleracea L. (2n = 18, CC) and their amphidiploid B. napus L. (2n = 38, AACC) are globally the most important. An understanding of the organization of the A and C genomes at the chromosomal and molecular levels in the diploids and the amphidiploid is fundamental for phylogenetic studies, manipulation in plant breeding, and the assessment of interspecific gene introgression in nature.Characterization of the A and C genomes and their relations to other genomes in Brassica and related genera has been the subject of many detailed studies that were primarily based on cytological, biochemical, or molecular analysis. Beginning with the classical work of U (1935), hybridization and cytogenetical analysis have elucidated the close relatedness of the A and C genomes, their intraand intergenome chromosome homoeologies, and their apparent secondary polyploid origin ( H A R - BERD and MCARTHUR 1980;INOMATA 1980;PRAKASH and HINATA 1980; ARMSTRONG and CIECHOWSKI 1985; ATTIA and R~BBELEN 1986a,b; ATTIA et al. 1987;TAI and IKONEN 1988;CHOPRA and PRAKASH 1991;CHENG et al. 1994b). Recently it has been possible to characterize the different chromosomes in the A and C genomes through the use of banding t...

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“…Molecular mapping of B. rapa was initiated by Song et al (1991) using RFLP markers and a segregating F 2 population. Subsequently several additional maps were constructed based on different molecular markers and F 2 , recombinant inbred lines (RILs) or DH populations (Chyi et al 1992;Teutonico and Osborn 1994;Kole et al 1997;Suwabe et al 2006;Kim et al 2006;Choi et al 2007). The recent development of Brassica simple sequence repeat (SSR) markers facilitates the anchoring of linkage groups to one common reference map and allows the comparison of map positions in studies involving different populations (Suwabe et al 2006;Choi et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Mapping QTLs for mineral accumulation and shoot dry biomass under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Yuan

Zhang

et al. 2008

Plant Soil

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Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most important vegetables in China. Genetic dissection of leaf mineral accumulation and tolerance to Zn stress is important for the improvement of the nutritional quality of Chinese cabbage by breeding. A mapping population with 183 doubled haploid (DH) lines was used to study the genetics of mineral accumulation and the growth response to Zn. The genetic map was constructed based on 203 AFLPs, 58 SSRs, 22 SRAPs and four ESTPs. The concentration of 11 minerals was determined in leaves for 142 DH lines grown in an open field. In addition shoot dry biomass (SDB) under normal, deficient and excessive Zn nutritional conditions were investigated in hydroponics experiments. Ten QTLs, each explaining 11.1-17.1% of the Na, Mg, P, Al, Fe, Mn, Zn and Sr concentration variance, were identified by multiple-QTL model (MQM) mapping. One common QTL was found affecting SDB under normal, deficient and excessive Zn nutritional conditions. An additional QTL was detected for SDB under Zn excess stress only. These Plant Soil (2008) 310:25-40

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A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn. campestris)

Cited by 106 publications

References 0 publications

A Sequence-Tagged Linkage Map ofBrassica rapa

A Sequence-Tagged Linkage Map ofBrassica rapa

Characterization of the A and C Genomes of Brassica Campestrisand B. Alboglabra

Mapping QTLs for mineral accumulation and shoot dry biomass under different Zn nutritional conditions in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

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