A Genetic Linkage Map of Atlantic Halibut (Hippoglossus hippoglossus L.)

Abstract

A genetic linkage map has been constructed for Atlantic halibut on the basis of 258 microsatellites and 346 AFLPs. Twenty-four linkage groups were identified, consistent with the 24 chromosomes seen in chromosome spreads. The total map distance is 1562.2 cM in the female and 1459.6 cM in the male with an average resolution of 4.3 and 3.5 cM, respectively. Using diploid gynogens, we estimated centromere locations in 19 of 24 linkage groups. Overall recombination in the female was approximately twice that of the male; however, this trend was not consistent along the linkage groups. In the centromeric regions, females had 11-17.5 times the recombination of the males, whereas this trend reversed toward the distal end with males having three times the recombination of the females. Correspondingly, in the male, markers clustered toward the centromeric region with 50% of markers within 20 cM of the putative centromere, whereas 35% of markers in the female were found between 60 and 80 cM from the putative centromere. Limited interspecies comparisons within Japanese flounder and Tetraodon nigroviridis revealed blocks of conservation in sequence and marker order, although regions of chromosomal rearrangement were also apparent.

Detection of Growth-Related Quantitative Trait Loci and High-Resolution Genetic Linkage Maps Using Simple Sequence Repeat Markers in the Kelp Grouper (Epinephelus bruneus)

“…Differences in recombination rates between sexes have been identified in many species; for example, humans (Dib et al 1996), dogs (Wong et al 2010), crocodiles (Miles et al 2009), and fish. In fish, recombination rates have generally been reported to be higher in females compared to males ranging from 3.25:1 in rainbow trout (Sakamoto et al 2000), 7.4:1 in the Japanese flounder (Coimbra et al 2003), 1.37:1 in Atlantic salmon (Lien et al 2011), 2.2:1 in the silver carp (Guo et al 2013), 2:1 in the Atlantic halibut (Reid et al 2007), 1.5:1 in the kelp grouper ), 1.03:1 in the orange-spotted grouper (You et al 2013), and 1.19:1 in the white grouper (Dor et al 2014). In this study, the recombination rate ratio between females and males was 1.12:1, which was lower than previous reports.…”

A Gene-Based Genetic Linkage Map of the Collared Flycatcher (Ficedula albicollis) Reveals Extensive Synteny and Gene-Order Conservation During 100 Million Years of Avian Evolution

“…In the two latter systems, microsatellite markers developed in closely related domestic animals could be used across species. In sticklebacks, anonymous, species-specific microsatellites were used and random approaches for map development have also included the use of microsatellites, RAPD, or AFLP markers in a number of other species (Reid et al 2007;Troggio et al 2007).The development of the collared flycatcher map represents an application of a new approach to genetic mapping in natural populations. For several reasons, it benefited from an earlier systematic effort to develop a set of conserved reference markers evenly spread across the avian genome (Backström et al 2008).…”

Advances in genomics for flatfish aquaculture

“…Moreover, genetic maps provide a useful tool to study genome organization and for gathering information about syntenic relationships across species and identification of putative candidate genes associated with QTLs (Castaño-Sanchez et al 2010;Sarropoulou et al 2008). Although genetic maps have been developed for turbot and Atlantic halibut (Reid et al 2007;Bouza et al 2007), the recent application of NGS has allowed the identification of a high number of genetic markers useful for high-density maps. In the turbot, for instance, the first generation map had 26 linkage groups (LG) based on 242 anonymous microsatellites and with an average marker distance of 6.5 centimorgan (cM) (Bouza et al 2007).…”