A general method for selection of riboflavin-overproducing food grade micro-organisms

Burgess, Catherine M.; Smid, Eddy J.; Rutten, Ger; Sinderen, Douwe van

doi:10.1186/1475-2859-5-24

Cited by 111 publications

(104 citation statements)

References 47 publications

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“…For instance, the sequenced genome of Lactobacillus plantarum strain WCFS1 had an incomplete rib operon, which lacked the entire ribG and part of the ribB genes [27]. Further, this strain could not grow unless riboflavin was present [28]. However, several selected strains of L. plantarum contained the whole rib operon and could produce vitamin B2.…”

Section: Riboflavin Biosynthesismentioning

confidence: 99%

“…However, several selected strains of L. plantarum contained the whole rib operon and could produce vitamin B2. The L. plantarum strain NCDO 1752, and the recently sequenced L. plantarum strain JDMI and L. plantarum strains, for example, were isolated from cereals-derived products [28,29]. Furthermore, even in LAB strains that contained all rib genes, riboflavin production had to be confirmed by chemical analysis.…”

Section: Riboflavin Biosynthesismentioning

confidence: 99%

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Biosynthesis of Vitamins by Probiotic Bacteria

Gu¹,

Li²

2016

Probiotics and Prebiotics in Human Nutrition and Health

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Vitamins are important micronutrients that are often precursors to enzymes, which all living cells require to perform biochemical reactions. However, humans cannot produce many vitamins, so they have to be externally obtained. Using vitamin-producing microorganisms could be an organic and marketable solution to using pseudo-vitamins that are chemically produced, and could allow for the production of foods with higher levels of vitamins that could reduce unwanted side effects. Probiotic bacteria, as well as commensal bacteria found in the human gut, such as Lactobacillus and Bifidobacterium, can de novo synthesize and supply vitamins to human body. In humans, members of the gut microbiota are able to synthesize vitamin K, as well as most of the water-soluble B vitamins, such as cobalamin, folates, pyridoxine, riboflavin, and thiamine.

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Section: Riboflavin Biosynthesismentioning

confidence: 99%

Section: Riboflavin Biosynthesismentioning

confidence: 99%

Biosynthesis of Vitamins by Probiotic Bacteria

Gu¹,

Li²

2016

Probiotics and Prebiotics in Human Nutrition and Health

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“…First, studies of microbes selected or engineered to overproduce riboflavin show that GTP cyclohydrolase II does not normally control flux in the pathway, and that GTP supply does [44–46]. Second, there are no reports of feedback inhibition of GTP cyclohydrolase II enzymes although they have been thoroughly studied [7].…”

Section: Discussionmentioning

confidence: 99%

A directed-overflow and damage-control N-glycosidase in riboflavin biosynthesis

Frelin

Huang

Hasnain

et al. 2015

Biochemical Journal

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Plants and bacteria synthesize the essential human micronutrient riboflavin (vitamin B2) via the same multistep pathway. The early intermediates of this pathway are notoriously reactive, and may be overproduced in vivo because riboflavin biosynthesis enzymes lack feedback controls. Here we demonstrate disposal of riboflavin intermediates by COG3236 (DUF1768), a protein of previously unknown function that is fused to two different riboflavin pathway enzymes in plants and bacteria (RIBR and RibA, respectively). We present cheminformatic, biochemical, genetic, and genomic evidence to show that: (i) plant and bacterial COG3236 proteins cleave the N-glycosidic bond of the first two intermediates of riboflavin biosynthesis, yielding relatively innocuous products; (ii) certain COG3236 proteins are in a multienzyme riboflavin biosynthesis complex that gives them privileged access to riboflavin intermediates; and (iii) COG3236 action in Arabidopsis thaliana and Escherichia coli helps maintain flavin levels. COG3236 proteins thus illustrate two emerging principles in chemical biology: directed overflow metabolism, in which excess flux is diverted out of a pathway, and the pre-emption of damage from reactive metabolites.

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“…Roseoflavin is a toxic riboflavin analog shown to select for spontaneous mutations in Bacillus subtilis strains with constitutive overproduction of riboflavin 38 . Several natural riboflavin overproducers have been described: Lactococcus lactis , 39 L. plantarum , Leuconosctoc mesenteroides , and Propionibacterium freudenreichii 40 , 41 . Microbially produced vitamins thus represent a possible means of fortifying foods with additional micronutrients.…”

Section: Micronutrient Absorptionmentioning

confidence: 99%

Can prebiotics and probiotics improve therapeutic outcomes for undernourished individuals?

et al. 2013

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It has become clear in recent years that the human intestinal microbiota plays an important role in maintaining health and thus is an attractive target for clinical interventions. Scientists and clinicians have become increasingly interested in assessing the ability of probiotics and prebiotics to enhance the nutritional status of malnourished children, pregnant women, the elderly, and individuals with non-communicable disease-associated malnutrition. A workshop was held by the International Scientific Association for Probiotics and Prebiotics (ISAPP), drawing on the knowledge of experts from industry, medicine, and academia, with the objective to assess the status of our understanding of the link between the microbiome and under-nutrition, specifically in relation to probiotic and prebiotic treatments for under-nourished individuals. These discussions led to four recommendations: (1) The categories of malnourished individuals need to be differentiated To improve treatment outcomes, subjects should first be categorized based on the cause of malnutrition, additional health-concerns, differences in the gut microbiota, and sociological considerations.(2) Define a baseline “healthy” gut microbiota for each category Altered nutrient requirement (for example, in pregnancy and old age) and individual variation may change what constitutes a healthy gut microbiota for the individual.(3) Perform studies using model systems to test the effectiveness of potential probiotics and prebiotics against these specific categories These should illustrate how certain microbiota profiles can be altered, as members of different categories may respond differently to the same treatment.(4) Perform robust well-designed human studies with probiotics and/or prebiotics, with appropriate, defined primary outcomes and sample size These are critical to show efficacy and understand responder and non-responder outcomes. It is hoped that these recommendations will lead to new approaches that combat malnutrition. This report is the result of discussion during an expert workshop titled “How do the microbiota and probiotics and/or prebiotics influence poor nutritional status?” held during the 10th Meeting of the International Scientific Association for Probiotics and Prebiotics (ISAPP) in Cork, Ireland from October 1–3, 2012. The complete list of workshop attendees is shown in Table 1.

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A general method for selection of riboflavin-overproducing food grade micro-organisms

Abstract: Background: This study describes a strategy to select and isolate spontaneous riboflavinoverproducing strains of Lactobacillus (Lb.) plantarum, Leuconostoc (Lc.) mesenteroides and Propionibacterium (P.) freudenreichii.

Cited by 111 publications

References 47 publications

Biosynthesis of Vitamins by Probiotic Bacteria

Biosynthesis of Vitamins by Probiotic Bacteria

A directed-overflow and damage-control N-glycosidase in riboflavin biosynthesis

Can prebiotics and probiotics improve therapeutic outcomes for undernourished individuals?

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