A general method for identifying major hybrid male sterility genes in Drosophila

Zeng, Lingwen; Singh, Rama S.

doi:10.1038/hdy.1995.144

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…For example, few nuclear loci should be monophyletic by chance in species of Echinometra, and thus any loci discovered that are monophyletic represent good candidates for the action of directional selection distinguishing species. This approach may provide a search criterion for candidate loci involved in speciation in closely related taxa for which, unlike Drosophila, defined introgressed lines cannot easily be created (Zeng and Singh 1995).…”

Section: A Spectrum Of Coalescence Ratiosmentioning

confidence: 99%

Predicting Nuclear Gene Coalescence From Mitochondrial Data: The Three-Times Rule

2007

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Abstract. Coalescence theory predicts when genetic drift at nuclear loci will result in fixation of sequence differences to produce monophyletic gene trees. However, the theory is difficult to apply to particular taxa because it hinges on genetically effective population size, which is generally unknown. Neutral theory also predicts that evolution of monophyly will be four times slower in nuclear than in mitochondrial genes primarily because genetic drift is slower at nuclear loci. Variation in mitochondrial DNA (mtDNA) within and between species has been studied extensively, but can these mtDNA data be used to predict coalescence in nuclear loci? Comparison of neutral theories of coalescence of mitochondrial and nuclear loci suggests a simple rule of thumb. The ''three-times rule'' states that, on average, most nuclear loci will be monophyletic when the branch length leading to the mtDNA sequences of a species is three times longer than the average mtDNA sequence diversity observed within that species.A test using mitochondrial and nuclear intron data from seven species of whales and dolphins suggests general agreement with predictions of the three-times rule. We define the coalescence ratio as the mitochondrial branch length for a species divided by intraspecific mtDNA diversity. We show that species with high coalescence ratios show nuclear monophyly, whereas species with low ratios have polyphyletic nuclear gene trees. As expected, species with intermediate coalescence ratios show a variety of patterns. Especially at very high or low coalescence ratios, the threetimes rule predicts nuclear gene patterns that can help detect the action of selection. The three-times rule may be useful as an empirical benchmark for evaluating evolutionary processes occurring at multiple loci. The application of molecular sequence data to systematics, population biology, and forensic identification of species often depends on whether monophyly has evolved at particular loci between taxa (Hudson 1992;Moritz 1994;Moore 1995;Palumbi and Cipriano 1998). Monophyly of genes is basic to some views of the species concept (Baum and Shaw 1995) and has been used to define evolutionarily significant units for management of threatened species (Moritz 1994). A population that has been separate long enough to develop gene monophyly has probably developed novel combinations of alleles at many genetic loci and may possess unique evolutionary features including adaptation to local environment that should be considered by resource managers (Moritz 1994). The shape of intra-and interspecific gene trees has also been used to infer the demographic history of species (Takahata 1995) and speciation patterns (M. P. Hare, F. Cipriano, and S. Palumbi, unpubl. ms.). Monophyly is also important if molecular tools are used in the identification of managed species in commercial markets (whales: Baker et al. 1993;Cipriano and Palumbi 1999; seals: Malik et al. 1997; canned fish: Rehbein et al. 1997; cannabis: Jagadish et al. 1996; caviar: Cohen 1997). This is...

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Section: A Spectrum Of Coalescence Ratiosmentioning

confidence: 99%

Predicting Nuclear Gene Coalescence From Mitochondrial Data: The Three-Times Rule

2007

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“…The male hybrid sterility is the most common post-zygotic reproductive isolation among related animal species, and, therefore, it has received special attention in the speciation studies (Zeng & Singh, 1995). In the last decade, the genetic basis of reproductive isolation has been shown to be surprisingly polygenic and recently several studies of reproductive isolation have been realized using molecular analysis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the courtship and of the hybrid male sterility among Drosophila buzzatii cluster species (Diptera, Drosophilidae)

2002

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In the Drosophila repleta group the establishment of subgroups and complexes made on the basis of morphological and cytological evidences is supported by tests of reproductive isolation. Among species in the repleta group, the buzzatii cluster, due to its polymorphism and polytipism, is an excellent material for ecological and speciation studies. Some interspecific crosses involving Drosophila seriema, Drosophila sp. B, D. koepferae and D. buzzatii strains were completely sterile while others involving strains from these species produced F1 hybrids that did not yield F2. In the present work, data on courtship duration and copula occurrence obtained in the analysis of flies from parental sterile crosses and on spermatozoon mobility observed in F1 hybrids that did not yield F2 are presented. Copula did not occur during one hour of observation and the spermatozoon also did not show mobility at any of the analyzed stages (3, 7, 9 and 10 days old). There was a high variation in courtship average duration and in the percentage of males that courted the females. The reproductive isolation mechanisms indicated by these observations were pre and post-zygotic, as supported by the absence of copula and male sterility. Data obtained also showed the occurrence of different degrees of reproductive compatibility among the strains classified as the same species but from distinct geographic localities.

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“…Therefore, cytoplasmic factors are not involved in hybrid male sterility in the cross between D. pseudoananassae and D. malerkotliana. Zeng and Singh (1995) have introduced a scheme to determine the role of any X-linked or dominant autosomal MHS genes in hybrid male sterility, which is shown in Fig. 2.…”

Section: Pseudoananassae and D Parabipectinata Species Pairmentioning

confidence: 99%

Genetic interactions underlying hybrid male sterility in the Drosophila bipectinata species complex

Mishra

Singh

2006

Genes Genet. Syst.

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Understanding genetic mechanisms underlying hybrid male sterility is one of the most challenging problems in evolutionary biology especially speciation. By using the interspecific hybridization method roles of Y chromosome, Major Hybrid Sterility (MHS) genes and cytoplasm in sterility of hybrid males have been investigated in a promising group, the Drosophila bipectinata species complex that consists of four closely related species: D. pseudoananassae, D. bipectinata, D. parabipectinata and D. malerkotliana . The interspecific introgression analyses show that neither cytoplasm nor MHS genes are involved but X-Y interactions may be playing major role in hybrid male sterility between D. pseudoananassae and the other three species. The results of interspecific introgression analyses also show considerable decrease in the number of males in the backcross offspring and all males have atrophied testes. There is a significant positive correlation between sex -ratio distortion and severity of sterility in backcross males. These findings provide evidence that D. pseudoananassae is remotely related with other three species of the D. bipectinata species complex.

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A general method for identifying major hybrid male sterility genes in Drosophila

Cited by 7 publications

References 41 publications

Predicting Nuclear Gene Coalescence From Mitochondrial Data: The Three-Times Rule

Predicting Nuclear Gene Coalescence From Mitochondrial Data: The Three-Times Rule

Evaluation of the courtship and of the hybrid male sterility among Drosophila buzzatii cluster species (Diptera, Drosophilidae)

Genetic interactions underlying hybrid male sterility in the Drosophila bipectinata species complex

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