A functional variant of the long noncoding RNA AL110200 is associated with the risk of ischaemic stroke recurrence

Abstract

Background and purpose: This study aimed to test the hypothesis that long noncoding RNA (lncRNA) AL110200 exerts a proinflammatory effect on atherosclerosis and that the variant rs901681 contributes to ischaemic stroke incidence and recurrence. Methods:The expression of AL110200 was analyzed in THP-1 cells treated with oxidized low-density lipoprotein and in human peripheral blood in a coronary heart disease and control population to determine the role of AL110200 in atherosclerosis. The effect of AL110200 on cell adhesion and invasion was tested. The plasma level of leukotriene B4 and rs901681 genotype distribution were assessed in 220 participants. In 1004 ischaemic stroke patients and 1434 controls, the association between rs901681 and stroke incidence was analyzed by logistic regression, and the association of rs901681 and stroke prognosis was analyzed using Kaplan-Meier analysis and the Cox proportional hazards model.Results: Increased expression of AL110200 was observed in THP-1 cells under oxidized low-density lipoprotein treatment. Knockdown of AL110200 reduced the adhesive and invasive ability of THP-1 cells. AL110200 expression in peripheral blood was significantly higher in the coronary heart disease group than in the controls. The GG genotype of rs901681 is associated with reduced plasma leukotriene B4. In the ischaemic stroke population, rs901681 was not associated with ischaemic stroke incidence (p = 0.686).Patients carrying rs901681 GG had a lower risk for stroke recurrence at age ≥60 years (p = 0.001), cardiovascular stroke death (p = 0.022) and all-cause mortality (p = 0.034) in the all-age group.Conclusions: AL110200 might exert a proinflammatory effect on atherosclerosis, and the variant rs901681 might be a strong predictor of stroke prognosis in ischaemic stroke patients.

Neutrophil count multiplied by D-dimer combined with pneumonia may better predict short-term outcomes in patients with acute ischemic stroke

“…NDM were divided into 5 quintiles, and multivariate regression analysis was conducted between NDM (quintile), Babinski sign+, pneumonia, cardiac disease and poor outcomes of AIS. The first grade of NDM (quintile) was < = 1.82, The second grade NDM (quintile) (1) was 1.83-2.41, The third grade NDM (quintile) (2) was 2.42-3.27, The fourth grade NDM (quintile) (3) was 3.28-4.49, The fifth grade NDM (quintile) (4) was 4.95+. The first grade of NDM (quintile) was the categorical covariable.…”

“…Acute ischemic stroke (AIS) is a disease with high morbidity and mortality, causes considerable disability-adjusted life-years worldwide [1,2]. More seriously, numerous AIS patients have poor short-term outcomes after the onset of disease.…”

SNHG15 is a negative regulator of inflammation by mediating TRAF2 ubiquitination in stroke-induced immunosuppression

“…Long noncoding RNAs (lncRNAs) are a set of non-protein-coding transcripts greater than 200 bases in length [ 12 ], and are involved in a series of physiological and pathological processes, including cell differentiation, embryonic development and regulation of the cell cycle [ 13 ]. A number of lncRNAs have been reported to have diagnostic and therapeutic values in ischemic stroke [ 14 – 16 ]. Our previous study showed differential expression of lncRNAs in peripheral blood mononuclear cells (PBMCs) in acute ischemic stroke, revealing that a panel of altered lncRNAs (linc-DHFRL1-4, SNHG15, and linc-FAM98A-3) may serve as a novel diagnostic tool [ 17 ].…”

New Drug Targets to Prevent Death Due to Stroke: A Review Based on Results of Protein-Protein Interaction Network, Enrichment, and Annotation Analyses

“…A review of the existing literature shows that a number of genes may increase risk of mortality due to IS, including SERPINE1 (serine protein inhibitor E1) or plasminogen activator inhibitor 1 (PAI-1), ITGB3 (integrin beta-3), MPG (DNA-3-methyladenine glycosylase), PROCR gene (soluble endothelial protein C receptor), HABP2 (Hyaluronan Binding Protein 2), COL3A1 (type III collagen), FBG, RETN (resistin), VKORC1 (Vitamin K epoxide reductase complex subunit 1), INADL (InaD-Like Protein, PALS1-Associated Tight Junction Protein), and FXIIIA Val34Leu genetic variant [30][31][32][33][34][35][36][37][38][39][40] Moreover, the micro-RNA (miR)-10a rs3809783 A>T (MIR10A) and miR-34b/c rs4938723 T>C genetic variant (MIR34B) and the long noncoding RNA (lncRNA) AL110200 are associated with increased mortality rates due to IS [41,42].…”