A Functional Link between Mitochondria and the Cell Wall in Stress Responses

Hofmann, Nancy R.

doi:10.1105/tpc.16.00691

Cited by 6 publications

(5 citation statements)

References 5 publications

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“…The plant cell wall is the first site to perceive and respond to abiotic stress 51 . The stress signal triggers cell wall remodelling so as to maintain the flexibility and protect the cell against ionic imbalance 52 . Major genes involved in cell wall organisation are dirigent protein, phosphatidylinositol phosphate kinase and lipid transfer proteins.…”

Section: Resultsmentioning

confidence: 99%

Differential Regulation of Genes Involved in Root Morphogenesis and Cell Wall Modification is Associated with Salinity Tolerance in Chickpea

Kaashyap

Ford

Kudapa

et al. 2018

Sci Rep

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Salinity is a major constraint for intrinsically salt sensitive grain legume chickpea. Chickpea exhibits large genetic variation amongst cultivars, which show better yields in saline conditions but still need to be improved further for sustainable crop production. Based on previous multi-location physiological screening, JG 11 (salt tolerant) and ICCV 2 (salt sensitive) were subjected to salt stress to evaluate their physiological and transcriptional responses. A total of ~480 million RNA-Seq reads were sequenced from root tissues which resulted in identification of 3,053 differentially expressed genes (DEGs) in response to salt stress. Reproductive stage shows high number of DEGs suggesting major transcriptional reorganization in response to salt to enable tolerance. Importantly, cationic peroxidase, Aspartic ase, NRT1/PTR, phosphatidylinositol phosphate kinase, DREB1E and ERF genes were significantly up-regulated in tolerant genotype. In addition, we identified a suite of important genes involved in cell wall modification and root morphogenesis such as dirigent proteins, expansin and casparian strip membrane proteins that could potentially confer salt tolerance. Further, phytohormonal cross-talk between ERF and PIN-FORMED genes which modulate the root growth was observed. The gene set enrichment analysis and functional annotation of these genes suggests they may be utilised as potential candidates for improving chickpea salt tolerance.

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Section: Resultsmentioning

confidence: 99%

Differential Regulation of Genes Involved in Root Morphogenesis and Cell Wall Modification is Associated with Salinity Tolerance in Chickpea

Kaashyap

Ford

Kudapa

et al. 2018

Sci Rep

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“…At a first glance, the role of β-glucans as a putative predictor of mediation seems suspicious. However, studies on cell wall signaling suggest that mitochondria and the cell wall cooperate to mediate stress responses [64]. Stresses can also affect the rate of respiratory electron transport, and mitochondria act as hubs for signaling to other cellular organelles [65].…”

Section: Discussionmentioning

confidence: 99%

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Bednarek

Żebrowski

Orłowska

2020

IJMS

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Tissue culture is an essential tool for the regeneration of uniform plant material. However, tissue culture conditions can be a source of abiotic stress for plants, leading to changes in the DNA sequence and methylation patterns. Despite the growing evidence on biochemical processes affected by abiotic stresses, how these altered biochemical processes affect DNA sequence and methylation patterns remains largely unknown. In this study, the methylation-sensitive Amplified Fragment Length Polymorphism (metAFLP) approach was used to investigate de novo methylation, demethylation, and sequence variation in barley regenerants derived by anther culture. Additionally, we used Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) spectroscopy to identify the spectral features of regenerants, which were then analyzed by mediation analysis. The infrared spectrum ranges (710–690 and 1010–940 cm−1) identified as significant in the mediation analysis were most likely related to β-glucans, cellulose, and S-adenosyl-L-methionine (SAM). Additionally, the identified compounds participated as predictors in moderated mediation analysis, explaining the role of demethylation of CHG sites (CHG_DMV) in in vitro tissue culture-induced sequence variation, depending on the duration of tissue culture. The data demonstrate that ATR-FTIR spectroscopy is a useful tool for studying the biochemical compounds that may affect DNA methylation patterns and sequence variation, if combined with quantitative characteristics determined using metAFLP molecular markers and mediation analysis. The role of β-glucans, cellulose, and SAM in DNA methylation, and in cell wall, mitochondria, and signaling, are discussed to highlight the putative cellular mechanisms involved in sequence variation.

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“…Among these correlated proteins, desiccation‐related PCC13‐62‐like protein (DCP) showed significant upregulation. In plants, stress signals perceived by the cell induce cell wall remodeling to sustain flexibility and maintain ionic homeostasis (Hofmann, 2016). Differential expression of DCP in the present study indicates that tolerant genotypes undertook cell wall remodeling and membrane stabilization to protect them from the adverse effects of drought stress.…”

Section: Discussionmentioning

confidence: 99%

Integrated multi‐omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)

et al. 2023

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Drought is one of the major constraints limiting chickpea productivity. To unravel complex mechanisms regulating drought response in chickpea, we generated transcriptomics, proteomics, and metabolomics datasets from root tissues of four contrasting drought‐responsive chickpea genotypes: ICC 4958, JG 11, and JG 11+ (drought‐tolerant), and ICC 1882 (drought‐sensitive) under control and drought stress conditions. Integration of transcriptomics and proteomics data identified enriched hub proteins encoding isoflavone 4′‐O‐methyltransferase, UDP‐d‐glucose/UDP‐d‐galactose 4‐epimerase, and delta‐1‐pyrroline‐5‐carboxylate synthetase. These proteins highlighted the involvement of pathways such as antibiotic biosynthesis, galactose metabolism, and isoflavonoid biosynthesis in activating drought stress response mechanisms. Subsequently, the integration of metabolomics data identified six metabolites (fructose, galactose, glucose, myoinositol, galactinol, and raffinose) that showed a significant correlation with galactose metabolism. Integration of root‐omics data also revealed some key candidate genes underlying the drought‐responsive “QTL‐hotspot” region. These results provided key insights into complex molecular mechanisms underlying drought stress response in chickpea.

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A Functional Link between Mitochondria and the Cell Wall in Stress Responses

Cited by 6 publications

References 5 publications

Differential Regulation of Genes Involved in Root Morphogenesis and Cell Wall Modification is Associated with Salinity Tolerance in Chickpea

Differential Regulation of Genes Involved in Root Morphogenesis and Cell Wall Modification is Associated with Salinity Tolerance in Chickpea

Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture

Integrated multi‐omics analysis reveals drought stress response mechanism in chickpea (Cicer arietinum L.)

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