A FRAP-based evaluation of protein diffusion in polyelectrolyte multilayers

Sustr, David; Duschl, Claus; Volodkin, Dmitry

doi:10.1016/j.eurpolymj.2015.03.063

Cited by 24 publications

(24 citation statements)

References 23 publications

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“…In further studies, this approach was mainly applied for studying the diffusion of proteins in HA/PLL PEMs [ 108 , 109 , 110 ]. It was found that, similarly to the polymers that comprise PEMs, proteins loaded into PEMs also represent several fractions that have different degree of the binding to polymer network and therefore have different diffusivity ( Figure 4 B) [ 109 ].…”

Section: Analysis Of Polymer Dynamics Inside Pems: Methodsmentioning

confidence: 99%

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Campbell

Vikulina

2020

Polymers

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Rapid development of versatile layer-by-layer technology has resulted in important breakthroughs in the understanding of the nature of molecular interactions in multilayer assemblies made of polyelectrolytes. Nowadays, polyelectrolyte multilayers (PEM) are considered to be non-equilibrium and highly dynamic structures. High interest in biomedical applications of PEMs has attracted attention to PEMs made of biopolymers. Recent studies suggest that biopolymer dynamics determines the fate and the properties of such PEMs; however, deciphering, predicting and controlling the dynamics of polymers remains a challenge. This review brings together the up-to-date knowledge of the role of molecular dynamics in multilayers assembled from biopolymers. We discuss how molecular dynamics determines the properties of these PEMs from the nano to the macro scale, focusing on its role in PEM formation and non-enzymatic degradation. We summarize the factors allowing the control of molecular dynamics within PEMs, and therefore to tailor polymer multilayers on demand.

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Section: Analysis Of Polymer Dynamics Inside Pems: Methodsmentioning

confidence: 99%

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Campbell

Vikulina

2020

Polymers

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“…However, more deep assessment of the effect of the chemical modification of the multilayers is required for better evaluation of the impact of the chemical cues such as fibrionectin adsorption to the multilayers. This is an issue to be considered in our future research based on a set of physicochemical instruments and approaches for the characterization of the multilayers we currently employ such as probing the molecular diffusion into the multilayers and imaging …”

Section: Resultsmentioning

confidence: 99%

“…This is an issue to be considered in our future research based on a set of physicochemical instruments and approaches for the characterization of the multilayers we currently employ such as probing the molecular diffusion into the multilayers and imaging. [40,55,66] When properly exploited, this extended "cell-friendly window" can be used for targeted release (making use of the reservoir properties of the film) to locally deliver biomolecules to desired cells. Bioactive molecules can be loaded directly into the preformed PEMs (postloading) [38] or trapped as constituents of the film during the LbL assembly process (embedding).…”

Section: Future Perspectivesmentioning

confidence: 99%

A “Cell‐Friendly” Window for the Interaction of Cells with Hyaluronic Acid/Poly‐l‐Lysine Multilayers

Madaboosi

Uhlig

Schmidt

et al. 2017

Macromolecular Bioscience

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Polyelectrolyte multilayers assembled from hyaluronic acid (HA) and poly-l-lysine (PLL) are most widely studied showing excellent reservoir characteristics to host molecules of diverse nature; however, thick (HA/PLL) films are often found cell repellent. By a systematic study of the adhesion and proliferation of various cells as a function of bilayer number "n" a correlation with the mechanical and chemical properties of films is developed. The following cell lines have been studied: mouse 3T3 and L929 fibroblasts, human foreskin primary fibroblasts VH-Fib, human embryonic kidney HEK-293, human bone cell line U-2-OS, Chinese hamster ovary CHO-K and mouse embryonic stem cells. All cells adhere and spread well in a narrow "cell-friendly" window identify in the range of n = 12-15. At n < 12, the film is inhomogeneous and at n > 15, the film is cell repellent for all cell lines. Cellular adhesion correlates with the mechanical properties of the films showing that softer films at higher "n" number exhibiting a significant decrease of the Young's modulus below 100 kPa are weakly adherent to cells. This trend cannot be reversed even by coating a strong cell-adhesive protein fibronectin onto the film. This indicates that mechanical cues plays a major role for cell behavior, also in respect to biochemical ones.

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“…This opens new avenues for the use of the multilayers as artificial extracellular matrices. Up to now the analysis of the protein-multilayer interactions has been done at room temperature [67][68][69] but not at a physiologically relevant temperature (37 1C). The mechanism of the protein-multilayer interaction is still unclear and first attempts to understand it showed that the PLL diffusion into the film may be responsible for the protein diffusion, 67 however, a deeper understanding will be concerned in our future studies.…”

Section: Discussionmentioning

confidence: 99%

Temperature effect on the build-up of exponentially growing polyelectrolyte multilayers. An exponential-to-linear transition point

Vikulina

Anissimov

Singh

et al. 2016

Phys. Chem. Chem. Phys.

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In this study, the effect of temperature on the build-up of exponentially growing polyelectrolyte multilayer films was investigated. It aims at understanding the multilayer growth mechanism as crucially important for the fabrication of tailor-made multilayer films. Model poly(L-lysine)/hyaluronic acid (PLL/HA) multilayers were assembled in the temperature range of 25-85 °C by layer-by-layer deposition using a dipping method. The film growth switches from the exponential to the linear regime at the transition point as a result of limited polymer diffusion into the film. With the increase of the build-up temperature the film growth rate is enhanced in both regimes; the position of the transition point shifts to a higher number of deposition steps confirming the diffusion-mediated growth mechanism. Not only the faster polymer diffusion into the film but also more porous/permeable film structure are responsible for faster film growth at higher preparation temperature. The latter mechanism is assumed from analysis of the film growth rate upon switching of the preparation temperature during the film growth. Interestingly, the as-prepared films are equilibrated and remain intact (no swelling or shrinking) during temperature variation in the range of 25-45 °C. The average activation energy for complexation between PLL and HA in the multilayers calculated from the Arrhenius plot has been found to be about 0.3 kJ mol(-1) for monomers of PLL. Finally, the following processes known to be dependent on temperature are discussed with respect to the multilayer growth: (i) polymer diffusion, (ii) polymer conformational changes, and (iii) inter-polymer interactions.

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A FRAP-based evaluation of protein diffusion in polyelectrolyte multilayers

Cited by 24 publications

References 23 publications

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

Layer-By-Layer Assemblies of Biopolymers: Build-Up, Mechanical Stability and Molecular Dynamics

A “Cell‐Friendly” Window for the Interaction of Cells with Hyaluronic Acid/Poly‐l‐Lysine Multilayers

Temperature effect on the build-up of exponentially growing polyelectrolyte multilayers. An exponential-to-linear transition point

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