A formalin-inactivated vaccine provides good protection against Vibrio harveyi infection in orange-spotted grouper ( Epinephelus coioides )

Nguyen, Hai Trong; Nguyen, Thuy Thi Thu; Tsai, Ming‐An; Ya-Zhen, E.; Wang, Pei-Chyi; Chen, Shih‐Chu

doi:10.1016/j.fsi.2017.04.008

Cited by 52 publications

(28 citation statements)

References 36 publications

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“…However, only the rOmpK‐OmpU‐vaccinated group showed a significant upregulation of these pro‐inflammatory cytokines as compared to the ISA‐injected fish, while the single protein rOmpK or rOmpU treatment groups showed a tendentious increase in pro‐inflammatory cytokines ( p < 0.1). The enhancement of IL‐1β and IL‐8 expression following vaccination is consistent with that observed in previous studies (Caipang, Hynes, Puangkaew, Brinchmann, & Kiron, ; Fast, Johnson, & Jones, ; Nguyen, Nguyen, Tsai et al., ). IL‐1β initiates inflammatory responses, and primes leucocytes for respiratory burst and phagocytosis following exposure to bacterial pathogens; this gene was immediately upregulated in the Atlantic cod after vaccination (Caipang et al., ).…”

Section: Discussionsupporting

confidence: 91%

“…The virulent V. harveyi strain AOD99026‐2sk5 ( Vh ) identified in our previous study (Nguyen, Nguyen, Tsai et al., ), V. parahaemolyticus strain OT‐105002‐434 ( Vp ) and V. alginolyticus strain OT103002‐273 ( Va ) were isolated from diseased groupers and cultured on tryptic soy agar (Difco, France) supplemented with 1.5% sodium chloride (TSN). The plasmid pET151 directional TOPO expression kit was obtained from Invitrogen (USA).…”

Section: Methodsmentioning

confidence: 99%

“…The specific primers used for RT‐qPCR in this study are listed in Table . The β‐actin gene, shown to be the most stable reference gene, has also been widely used for studying immune gene expression in groupers; thus, it was utilized as an internal control gene (Dan, Zhang, Li, & Li, ; Huang et al., ; Nguyen, Nguyen, Wang, Wang, & Chen, ; Nguyen, Nguyen, Tsai et al., ). RT‐qPCR was performed in the CFX96 Touch™ Real‐Time PCR Detection System (Bio‐Rad, USA) using the iQSYBR Green Supermix (Bio‐Rad, USA) in 10‐μl reaction volumes, and each sample was run in duplicates.…”

Section: Methodsmentioning

confidence: 99%

“…Six weeks after PI, the V. harveyi rOmpK‐, rOmpU‐, rOmpK‐OmpU‐, ISA‐ and PBS‐injected fishes ( n = 20) were intraperitoneally injected with 0.1 ml of the Vh strain (1.6 × 10 8 CFU/ml) (Nguyen, Nguyen, Wang et al., ; Nguyen, Nguyen, Tsai et al., ); testing was carried out in duplicate. An additional group of twenty groupers were kept in the same culturing system as a nonchallenge control group.…”

Section: Methodsmentioning

confidence: 99%

“…The development of a vaccine against vibriosis has been successful to some degree. Different types of vaccines have been prepared and tested, including inactivated (Nguyen, Nguyen, Tsai et al., ), recombinant (Li et al., ), attenuated (Hu, Cheng, Zhang, & Sun, ) and DNA (Hu & Sun, ) vaccines; most of them showed good protective capacity in experimental trials. However, these vaccines either contained mineral oil adjuvants or had a rather narrow protection spectrum against specific bacterial strains; none of them have been commercialized.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Nguyen

Chen

et al. 2018

Journal of Fish Diseases

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Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable Montanide ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization. Notably, enhanced immune responses and significant protective efficacy against V. harveyi infections were observed in the fusion protein vaccine-injected fishes with relative per cent survival value of 81.8%. Additionally, the rOmpK-OmpU antisera presented a high bactericidal effect on not only V. harveyi, but also Vibrio parahaermolyticus and Vibrio alginolyticus. Our results demonstrated that the fusion protein rOmpK-OmpU was an effective vaccine candidate that exhibited potentially great versatility for controlling vibrio infections.

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Section: Discussionsupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Nguyen

Chen

et al. 2018

Journal of Fish Diseases

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Vibriosis in Fish: A Review on Disease Development and Prevention

et al. 2018

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Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease . Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed. 4INA-SALWANY ET AL.

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Immuno-Protective Efficiency of the Bivalent Inactivated Vaccine Against Vibrio scophthalmi and Aeromonas salmonicida Infections in Turbot (Scophthalmus maximus L.)

Zhou¹,

Zheng²,

Ding³

et al. 2023

J. Ocean Univ. China

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A formalin-inactivated vaccine provides good protection against Vibrio harveyi infection in orange-spotted grouper ( Epinephelus coioides )

Cited by 52 publications

References 36 publications

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Vibriosis in Fish: A Review on Disease Development and Prevention

Immuno-Protective Efficiency of the Bivalent Inactivated Vaccine Against Vibrio scophthalmi and Aeromonas salmonicida Infections in Turbot (Scophthalmus maximus L.)

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