A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin

Abstract

11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.

Structural Mechanism of Ring-opening Reaction of Glucose by Human Serum Albumin

“…The rHSA⅐glucose and rHSA⅐fructose complexes were prepared at a molar ratio of 1:15. The final HSA concentrations in the complexes were about 100 mg/ml, similar to previous studies (22)(23)(24). The complex was then crystallized by sitting drop vapor diffusion at room temperature by mixing 1 l of the protein complex with 1 l of the precipitant solution containing 25-30% (w/v) polyethylene glycol 3350 in 50 mM potassium phosphate at pH 7.5.…”

Fatty acid binding to serum albumin: Molecular simulation approaches

“…Fusidic acid [21], oxyphenbutazone [17], propofol [9] 6 DAUDA d [29], diflunisal [17], halothane [9], ibuprofen [17] 7…”

“…Aspirin [18], azapropazone [17], AZT b [20], citric acid [16], CMPF c [17], dansyl-L-arginine [26], dansyl-L-asparagine [26], dansyl-L-glutamate [26], dansyl-L-phenylalanine [26], DAUDA d [29], diflunisal [17], halothane [9], indomethacin [17], indoxyl sulfate [17], iodipamide [17], iophenoxic acid [27], lysophosphatidylethanolamine [22], oxyphenbutazone [17], phenylbutazone [17], salicylic acid [18,20], triiodobenzoic acid [7], warfarin [12,17] a The numbering of the FA binding sites was sourced from Bhattacharya et al [10]. b 3'-Azido-3'-deoxythymidine.…”

Mechanistic investigation of domain specific unfolding of human serum albumin and the effect of sucrose

“…The complete understandings of the unfolding process require a detailed domain specific study and thus establish the complete denaturation landscape. Human serum albumin (HSA, Scheme ) is such a multidomain protein with three different distinguishable domains and has been studied widely in the last few decades . It consists of 585 amino acids residue and is the major protein found in our circulatory system .…”

“…Human serum albumin (HSA, Scheme 1) is such a multidomain protein with three different distinguishable domains and has been studied widely in the last few decades. [4][5][6][7][8][9][10][11][12][13][14] It consists of 585 amino acids residue and is the major protein found in our circulatory system. 15 The principal function of HSA is to transport a variety of metabolites and drugs in human body.…”