A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva

Verbeke, Frederick; borght, Kevin Van der; Spiegeleer, Anton De; Debunne, Nathan; Wynendaele, Evelien

doi:10.1016/j.jpba.2022.114594

Cited by 2 publications

(5 citation statements)

References 24 publications

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“…QS mechanisms are the basis of how bacteria react and regulate stress that can ensure survival and homeostasis of a functional bacterial community 17 , 57 . Both cortisol and glucose are known to interact with QS whilst individual taxa are able to counteract by releasing particular autoinducer peptides, which often enhances their virulence and growth-speed 56 , 58 , 59 . Furthermore, some microbial metabolites can neutralise the action of the QS peptides aiming to promote homeostasis within the community, such metabolites are D-Galactose and D-arabinose 60 .…”

Section: Discussionmentioning

confidence: 99%

The oral microbiome is associated with HPA axis response to a psychosocial stressor

Charalambous,

Mériaux,

Guebels

et al. 2024

Sci Rep

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Intense psychosocial stress during early life has a detrimental effect on health-disease balance in later life. Simultaneously, despite its sensitivity to stress, the developing microbiome contributes to long-term health. Following stress exposure, HPA-axis activation regulates the “fight or flight” response with the release of glucose and cortisol. Here, we investigated the interaction between the oral microbiome and the stress response. We used a cohort of 115 adults, mean age 24, who either experienced institutionalisation and adoption (n = 40) or were non-adopted controls (n = 75). Glucose and cortisol measurements were taken from participants following an extended socially evaluated cold pressor test (seCPT) at multiple time points. The cohort´s oral microbiome was profiled via 16S-V4 sequencing on microbial DNA from saliva and buccal samples. Using mixed-effect linear regressions, we identified 12 genera that exhibited an interaction with host’s cortisol-glucose response to stress, strongly influencing intensity and clearance of cortisol and glucose following stress exposure. Particularly, the identified taxa influenced the glucose and cortisol release profiles and kinetics following seCPT exposure. In conclusion, our study provided evidence for the oral microbiome modifying the effect of stress on the HPA-axis and human metabolism, as shown in glucose-cortisol time series data.

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Section: Discussionmentioning

confidence: 99%

The oral microbiome is associated with HPA axis response to a psychosocial stressor

Charalambous,

Mériaux,

Guebels

et al. 2024

Sci Rep

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“…For example, taking N-methylene-Cbf-14, even though the proton ions displayed identical m/z values ranging from 611.0705 to 611.0709 for impurities two, four, and six, the alkylidene sites in the Cbf-14 amino acid could be determined using secondary mass spectrometry. As shown in Figure 5, impurity two was identified as methylene-Cbf-14 at N-terminal due to the presence of a product ion at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)). Impurity four was recognized as methylene-Cbf-14 at K 9 , as evidenced by product ions at m/z 741.4825 (methylene-b10) and m/z 732.9859 ([methylene-b10]-NH 3 ), while lacking product ions at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)) or m/z 948.6092 (Cbf-14 (6-12)).…”

Section: Analyzing the Mechanisms Of Peptide Degradation Impurity For...mentioning

confidence: 99%

“…As shown in Figure 5, impurity two was identified as methylene-Cbf-14 at N-terminal due to the presence of a product ion at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)). Impurity four was recognized as methylene-Cbf-14 at K 9 , as evidenced by product ions at m/z 741.4825 (methylene-b10) and m/z 732.9859 ([methylene-b10]-NH 3 ), while lacking product ions at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)) or m/z 948.6092 (Cbf-14 (6-12)). Impurity six was identified as methylene-Cbf-14 at K 5 , supported by the presence of product ions at m/z 741.4816 (methylene-b10) and m/z 948.6092 (Cbf-14 (6-12)) but the absence of a product ion at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)).…”

Section: Analyzing the Mechanisms Of Peptide Degradation Impurity For...mentioning

confidence: 99%

“…Impurity four was recognized as methylene-Cbf-14 at K 9 , as evidenced by product ions at m/z 741.4825 (methylene-b10) and m/z 732.9859 ([methylene-b10]-NH 3 ), while lacking product ions at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)) or m/z 948.6092 (Cbf-14 (6-12)). Impurity six was identified as methylene-Cbf-14 at K 5 , supported by the presence of product ions at m/z 741.4816 (methylene-b10) and m/z 948.6092 (Cbf-14 (6-12)) but the absence of a product ion at m/z 640.9136 (Cbf-14 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)). Regarding N-(2hydroxyethylidene)-Cbf-14, impurities 10 and 11 were predicted based on the primary and secondary mass spectra information displayed in Figure 4b,d and electronic supplementary material (ESM, Figure S1g,h), respectively.…”

Section: Analyzing the Mechanisms Of Peptide Degradation Impurity For...mentioning

confidence: 99%

“…In alignment with International Conference on Harmonization (ICH) guidelines [ 10 ], impurities exceeding 0.1% of the API necessitate identification and characterization as part of a risk assessment, concerning their impact on patient safety and product efficacy. Typically, pharmaceutical research involving peptides employs various technologies, including liquid chromatography coupled with high-resolution mass spectrometry (LC–HRMS) [ 11 ], capillary electrophoresis (CE) [ 12 ], nuclear magnetic resonance (NMR) spectroscopy [ 13 ], circular dichroism (CD) spectroscopy [ 14 ], X-ray diffraction (XRD) [ 15 ], etc. During the development of Cbf-14 gel, an unusual degradation of APIs following long-term storage was encountered.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Compatibility Study of Peptide and Glycerol Using Chromatographic and Spectroscopic Techniques: Application to a Novel Antimicrobial Peptide Cbf-14 Gel

Yang,

Huo,

Jin

et al. 2023

Pharmaceutics

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The interactions between active pharmaceutical ingredients (APIs) and excipients may lead to API degradation, thereby affecting the safety and efficacy of drug products. Cbf-14 is a synthetic peptide derived from Cathelicidin-BF, showing potential for bacterial and fungal infections. In order to assess impurities in Cbf-14 gel, we developed a two-dimensional liquid chromatography coupled with quadrupole/time-of-flight mass spectrometric method. A total of eleven peptide degradation impurities were identified and characterized. Furthermore, the compatibility tests were conducted to evaluate the interactions of Cbf-14 with glycerol and methylcellulose, respectively. The results revealed that the impurities originated from condensation reactions between Cbf-14 and aldehydes caused by glycerol degradation. Several aldehydes were employed to validate this hypothesis. The formation mechanisms were elucidated as Maillard reactions between primary amino groups of Cbf-14 and aldehydes derived from glycerol degradation. Additionally, the compatibility of Cbf-14 with glycerol from different sources and with varying storage times was investigated. Notably, the interaction products in the gel increased with extended storage time, even when fresh glycerol for injection was added. This study offers unique insights into the compatibility study of peptides and glycerol, contributing to the ongoing quality study of Cbf-14 gel. It also serves as a reference for the design of other peptide preparations and excipients selections.

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A fit-for-purpose LC-MS/MS method for the analysis of selected Streptococcal quorum sensing peptides in human saliva

Cited by 2 publications

References 24 publications

The oral microbiome is associated with HPA axis response to a psychosocial stressor

The oral microbiome is associated with HPA axis response to a psychosocial stressor

Compatibility Study of Peptide and Glycerol Using Chromatographic and Spectroscopic Techniques: Application to a Novel Antimicrobial Peptide Cbf-14 Gel

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