A First Expression, Purification and Characterization of Endo-β-1,3-Glucanase from Penicillium expansum

Abstract

β-1,3-glucanase plays an important role in the biodegradation, reconstruction, and development of β-1,3-glucan. An endo-β-1,3-glucanase which was encoded by PeBgl1 was expressed, purified and characterized from Penicillium expansum for the first time. The PeBgl1 gene was amplified and transformed into the competent cells of E. coli Rosetta strain with the help of the pET-30a cloning vector. The recombinant protein PeBgl1 was expressed successfully at the induction conditions of 0.8 mmol/L IPTG at 16 °C for 16 h and then was purified by nickel ion affinity chromatography. The optimum reaction temperature of PeBgl1 was 55 °C and it had maximal activity at pH 6.0 according to the enzymatic analysis. Na2HPO4-NaH2PO4 buffer (pH 6.0) and NaCl have inhibitory and enhancing effects on the enzyme activities, respectively. SDS, TritonX-100 and some metal ions (Mg2+, Ca2+, Ba2+, Cu2+, and Zn2+) have an inhibitory effect on the enzyme activity. The results showed that PeBgl1 protein has good enzyme activity at 50–60 °C and at pH 5.0–9.0, and it is not a metal dependent enzyme, which makes it robust for storage and transportation, ultimately holding great promise in green biotechnology and biorefining.

Glucanase activity produced by rhizospheric Streptomyces tritolerans ARJ 32 and Streptomyces collinus ARJ 38 and the analysis of their encoding genes

“…The β-1,3-glucanase activity of Streptomyces spp. was measured by the dinitrosalicylic acid (DNS) method with slight modifications (Wang et al 2023). Streptomyces isolates were inoculated on liquid International Streptomyces Project (ISP4) medium (10 g soluble starch; 2 g (NH4)2SO4; 2 g CaCO3; 1 g K2HPO4; 1 g MgSO4•7H2O; 1 g NaCl; 0,001 FeSO4•7H2O; 0,001 g MnCl2; 0,001 g ZnSO4•7H2O) supplemented with 1% of βglucan (b/v) and incubated on a shaker at 120 rpm at room temperature for 14 days.…”