A First-Derivative Spectrophotometric Method for the Determination of Ciprofloxacin Hydrochloride in Ophthalmic Solution

Cazedey, Edith Cristina Laignier; Bonfílio, Rudy; Araújo, Magali Benjamim; Salgado, Hérida Regina Nunes

doi:10.5923/j.pc.20120206.06

Cited by 41 publications

(23 citation statements)

References 31 publications

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“…[62][63][64][65] However, it is a non-selective method and is not suitable for the detection of degradation products and impurities. [66] Spectrophotometric methods are also found in literature for other fluoroquinolones, such as ciprofloxacin, [67] norfloxacin, [68,69] orbifloxacin, [70] lomefloxacin, [71] gatifloxacin, [72] and sparfloxacin. [73,74] Costa et al (2014) developed and validated a microbiological agar diffusion method for the potency analysis of besifloxacin in ophthalmic suspension.…”

Section: Uv (289 Nm)mentioning

confidence: 99%

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Tótoli

Salgado

2018

Critical Reviews in Analytical Chemistry

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Bacterial conjunctivitis has high impact on the health of the population, since it represents more than a third of ocular pathologies reported by health services worldwide. There is a high incidence of bacterial resistance to the antimicrobials most commonly used for the treatment of conjunctivitis. In this context, besifloxacin stands out, since it is a fluoroquinolone developed exclusively for topical ophthalmic use, presenting a low risk of developing resistance due to its reduced systemic exposure. Bausch & Lomb markets it as ophthalmic suspension, under the trade name Besivance™. Literature review on besifloxacin is presented, covering its pharmaceutical and clinical characteristics, and the analytical methods used to measure the drug in pharmaceutical products and biological samples. High performance liquid chromatography is the most used method for this purpose. A discussion on Green Chemistry is also presented, focusing the importance of the development of green analytical methods for the analysis of drugs.

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Section: Uv (289 Nm)mentioning

confidence: 99%

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Tótoli

Salgado

2018

Critical Reviews in Analytical Chemistry

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“…Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-, 7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] a corticosteroid, is used to relieve bacterial ocular infections that require corticosteroid [1]. Many authors have reported a lot of different analytical methods for both drugs individually utilizing different techniques like HPLC [2][3][4][5][6][7][8][9], spectrophotometry and spectrofluorometric [10][11][12][13], titrimetric [14] methods for ciprofloxacin while for dexamethasone different methods involving HPLC, GC and TLC are reported [15][16][17][18][19][20][21].…”

Section: Ciprofloxacinmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Razzaq

Ashfaq

Mariam³

et al. 2017

J. Chil. Chem. Soc.

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A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min -1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL -1 for ciprofloxacin (r 2 ≥ 0.999) and 1-7 mg mL -1 for dexamethasone (r 2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture. 1-INTRODUCTION Ciprofloxacin(1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-quinoline-3-carboxylic acid), a well-known antibiotic, is used to relieve bacterial infections of the eyes, corneal ulcers along with some common bacterial attacks. Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10, 13,16-trimethyl-,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] also the enforcement agencies in general to monitor the quality of the marketed products. 2-EXPERIMENTAL Chemicals and ReagentsCiprofloxacin (99.87%) and dexamethasone (99.96%) reference standards were received from Schazoo Zaka Laboratories (Lahore, Pakistan) and were used as such without further refinement. Commercial products (Zoxan D and Ciprodex) containing 3 mg mL -1 ciprofloxacin and 1 mg mL -1 dexamethasone were analyzed during the current research work. All the chemicals used were of either HPLC grade or highest grade available commercially and were used as such. Double distilled water, other liquids used in mobile phase and all the injecting solutions were subject to filtration employing 0.45 mm nylon filters (Millipore, USA). Equipment and Chromatographic ConditionsAll the chromatographic work was conducted on LC-20A system (Shimadzu, Japan) using PDA detector at 270 nm. Each time 20 mL volume of analytes were injected using the fixed loop and peak areas of ciprofloxacin and dexamethasone were integrated using system built software. For separation, Hypersil BDS C8 column (250 X 4.6 mm, 5 mm) was used at room temperature. Pre-mixed mobile phase was used which was prepared by mixing 59:41 v/v ratio of methanol and 0.018M phosphate buffer (pH 3.0) and was flowe...

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“…at λ of 275 nm in aqueous solution [19][20]22]. The similarity in physicochemical properties makes LEV-CPR ratio remained constant throughout the sample preparation.…”

Section: Introductionmentioning

confidence: 98%

A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

et al. 2017

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A First-Derivative Spectrophotometric Method for the Determination of Ciprofloxacin Hydrochloride in Ophthalmic Solution

Cited by 41 publications

References 31 publications

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

A Rapid and Simple High-Performance Liquid Chromatographic Method for Determination of Levofloxacin in Human Plasma

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