A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

Rocha, Bruno Alves; Costa, Bruno Ruiz Brandão da; Albuquerque, Nayara Cristina Perez de; Oliveira, Anderson Rodrigo Moraes de; Souza, Juliana Maria Oliveira; Al-Tameemi, Maha; Campiglia, Andrés D.; Barbosa, Fernando

doi:10.1016/j.talanta.2016.03.098

Cited by 98 publications

(27 citation statements)

References 46 publications

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“…Further optimizations would be necessary to increase the sensitivity of our method such as adding an isolator column between the injector and the analytical column to prevent the BPS background contamination generated by the LC-MS/MS system [35] or using dansyl derivatization to improve the ionization and elution of BPS and BPS-G [36,37]. Nevertheless, this method is not designed for human biomonitoring and its LLOQs are totally suitable for our TK investigations.…”

Section: Sensitivitymentioning

confidence: 99%

“…Finally, we demonstrated the suitability of our analytical method for determining the time course of both BPS and BPS-G concentrations in plasma in the same run without resorting to enzymatic hydrolysis. This direct method offers two main advantages since it can be considered as more reliable than enzymatic hydrolysis and is also less time-consuming and definitely cheaper [15,37].…”

Section: 43)mentioning

confidence: 99%

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Development of an on-line solid phase extraction ultra-high-performance liquid chromatography technique coupled to tandem mass spectrometry for quantification of bisphenol S and bisphenol S glucuronide: Applicability to toxicokinetic investigations

Grandin

Picard‐Hagen

Gayrard

et al. 2017

Journal of Chromatography A

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Regulatory measures and public concerns regarding bisphenol A (BPA) have led to its replacement by structural analogues, such as Bisphenol S (BPS), in consumer products. At present, no toxicokinetic investigations have been conducted to assess the factors determining human internal exposure to BPS for subsequent risk assessment. Toxicokinetic studies require reliable analytical methods to measure the plasma concentrations of BPS and its main conjugated metabolite, BPS-glucuronide (BPS-G). An efficient on-line SPE-UPLC-MS/MS method for the simultaneous quantification of BPS and BPS-G in ovine plasma was therefore developed and validated in accordance with the European Medicines Agency guidelines for bioanalytical method validation. This method has a limit of quantification of 3ngmL for BPS and 10ngmL for BPS-G, an analytical capacity of 200 samples per day, and is particularly well suited to toxicokinetic studies. Use of this method in toxicokinetic studies in sheep showed that BPS, like BPA, is efficiently metabolized into its glucuronide form. However, the clearances and distributions of BPS and BPS-G were lower than those of the corresponding unconjugated and glucuroconjugated forms of BPA.

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Section: Sensitivitymentioning

confidence: 99%

Section: 43)mentioning

confidence: 99%

Development of an on-line solid phase extraction ultra-high-performance liquid chromatography technique coupled to tandem mass spectrometry for quantification of bisphenol S and bisphenol S glucuronide: Applicability to toxicokinetic investigations

Grandin

Picard‐Hagen

Gayrard

et al. 2017

Journal of Chromatography A

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“…LPME is a solvent-minimized sample pre-treatment compared to LLE. LPME can be categorized into three principal classes, single-drop microextraction (SDME), hollow-fibre protected liquid phase microextration (HF-LPME) (Kawaguchi et al 2008) and dispersive liquid-liquid microextraction (DLLME) (Cunha & Fernandes 2010;Liu et al 2013;Loh et al 2017;Rocha et al 2016). All these LPME techniques have been demonstrated to offer sensitive and selective detection of BPA in various sample matrices.…”

Section: Introductionmentioning

confidence: 99%

Simple Extraction of Bisphenol A in Beverages and Water by Membrane-Protected Liquid Phase Microextraction

Loh¹,

Khalik²,

Loh³

2020

JSM

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Bisphenol A (BPA) is a xenoestrogen that widely used in manufacture of plastics. Concern is mounting up when the application of BPA is widespread. A simple sample pre-treatment technique termed membrane-protected liquid phase microextraction (MP-LPME) combined with high performance liquid chromatography (HPLC)-fluorescence detection (FD) was developed for the determination of BPA in beverages and environmental water samples. The MP-LPME technique utilized a nylon membrane to hold the extractant (1-octanol) and prevent the extractant from dissolution into sample solution during agitation. Under optimal extraction conditions, the MP-LPME-HPLC-FD demonstrated ultratrace detection of BPA and insignificant matrix effect extraction with good relative recoveries in the range of 87.1-99.7% from spiked beverage and environmental water samples. The membrane can be reapplied in the MP-LPME after the first usage and offered relative recovery of > 94%. The MP-LPME technique is eco-friendly as it consumes only minute amounts of organic solvent which is supportive to green analysis.

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“…Bisphenol A (BPA) belongs to the large group of bisphenolic compounds. It is an important precursor in the production of phenol resins, polyesters, epoxy resins and polycarbonate plastics (Rocha et al, ). It can be found in milk bottles, internal protective coatings of metal food containers and paper products (Calafat, Ye, Wong, Reidy, & Needham, ; Liao & Kannan, ; Vandenberg, Hauser, Marcus, Olea, & Welshons, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of bisphenol A on gap junctions in HaCaT cells as mediated by the estrogen receptor pathway

Zhang

Liu

et al. 2018

J of Applied Toxicology

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Bisphenol A (BPA) is widely used as the raw material for the production of plastics and paper products. People can be exposed to BPA through dermal contact, particularly for cashiers in contact with thermal paper every day. BPA is a known endocrine disruptor that has been shown to be carcinogenic. Many tumors show weak gap junctional intercellular communication (GJIC) function. The aim of this study was to investigate the effects and possible mechanisms of BPA's action on GJIC of human HaCaT skin cells. The results showed that BPA increased cell proliferation rates, prolonged GJIC photobleaching fluorescence recovery times and reduced overall fluorescence recovery rates at 0.01, 0.1 and 1 μm. BPA downregulated connexin (Cx)26 mRNA level at 0.1 μm. Estrogen receptor (ER) antagonist ICI 182 780 at 5 nm partially blocked the above effects of BPA indicating involvement of the ER pathway with BPA exposure. However, BPA did not influence Cx43 mRNA and protein levels. Our immunofluorescence data showed that Cx26 was expressed in the cytoplasm and plasma membrane, and was involved in the formation of gap junctions between adjacent cells, while Cx43 was expressed only in the cytoplasm. Therefore, our data indicate that Cx26 gap junctions may be involved in the GJIC inhibition caused by BPA. In conclusion, our results indicate that BPA can promote human skin cell proliferation, inhibit skin cell GJIC function but not formation and downregulate Cx26 mRNA levels partially through the ER pathway. We hypothesize that BPA can exhibit carcinogenicity by inhibiting GJIC.

show abstract

A fast method for bisphenol A and six analogues (S, F, Z, P, AF, AP) determination in urine samples based on dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

Cited by 98 publications

References 46 publications

Development of an on-line solid phase extraction ultra-high-performance liquid chromatography technique coupled to tandem mass spectrometry for quantification of bisphenol S and bisphenol S glucuronide: Applicability to toxicokinetic investigations

Development of an on-line solid phase extraction ultra-high-performance liquid chromatography technique coupled to tandem mass spectrometry for quantification of bisphenol S and bisphenol S glucuronide: Applicability to toxicokinetic investigations

Simple Extraction of Bisphenol A in Beverages and Water by Membrane-Protected Liquid Phase Microextraction

Effects of bisphenol A on gap junctions in HaCaT cells as mediated by the estrogen receptor pathway

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