A facile and dynamic assay for the detection of peptide aggregation

Dong, Lin; Ren, Ruxia; Tan, Qianjing; Wu, Qiuyi; Li, Fangfang; Li, Lin; Liu, Shuwen

doi:10.1007/s00216-015-9271-4

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…To confirm this observation, the interaction between the bacterial membrane and C16‐KWKW was studied further by employing tryptophan fluorescence spectroscopic analysis (Caesar, Esbjorner, Lincoln, & Norden, ; Lin et al, ). In the fluorescence spectra, the amino acid tryptophan has a wavelength of maximum absorption of 280 nm and an emission peak ranging from approximately 300 to 350 nm depending on changes in the local environment.…”

Section: Resultsmentioning

confidence: 99%

Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with a dual antibacterial and anti‐inflammatory action

Yang

Wang

et al. 2019

British J Pharmacology

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Background and Purpose Propionibacterium acnes is a Gram‐positive bacterium associated with the skin disorder acne. In this study, as fatty acids are considered to be important in the life habitat of P. acnes, we tested our lipopeptide library in an attempt to create potent P. acnes‐specific antimicrobial agents. Experimental Approach The antimicrobial activity of various lipopeptides was determined by measuring their minimal inhibitory concentration (MIC). Lipids from P. acnes were used to explore their mode of action. RAW264.7 cells stimulated with LPS and P. acnes respectively were used to measure their anti‐inflammatory activity. Mice ears injected with P. acnes were used to assess the antimicrobial and anti‐inflammatory effects of the peptides tested in vivo. Key Results The most potent candidate, C16‐KWKW, was observed to be more active against P. acnes than against other non‐targeted bacterial strains, such as Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. The mode of action of C16‐KWKW was observed to be through interference with the integrity of the bacterial membrane, thereby impairing membrane permeability and causing leakage of inner contents of bacterial cells. Furthermore, C16‐KWKW inhibited the expression of pro‐inflammatory cytokines, such as IL‐1β, TNF‐α, and inducible NOS stimulated by both LPS and P. acnes, thus showing potential anti‐inflammatory activity, which was further verified in the in vivo animal studies. Conclusions and Implications C16‐KWKW is a lipopeptide displaying both anti‐P. acnes and anti‐inflammatory effects in vitro and in vivo and shows potential as a treatment for acne vulgaris induced by P. acnes.

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Section: Resultsmentioning

confidence: 99%

Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with a dual antibacterial and anti‐inflammatory action

Yang

Wang

et al. 2019

British J Pharmacology

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“…To confirm this observation, the interaction between the bacterial membrane and C16-KWKW was further studied by employing tryptophan fluorescence spectroscopic analysis (Caesar, Esbjorner, Lincoln, & Norden, 2006;Lin et al, 2016). In the fluorescence spectra, the amino acid tryptophan has a wavelength of maximum absorption of 280 nm and an emission peak ranging from 300 to 350 nm depending on the change in the local environment.…”

Section: R E T R a C T E Dmentioning

confidence: 98%

Retracted: Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with dual antibacterial and anti‐inflammatory action

Yang

Wang

et al. 2019

British J Pharmacology

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Background and Purpose: Propionibacterium acnes (P. acnes) is a Gram-positive bacterium associated with the skin disorder acne. In this study, we determined the importance of fatty acids in the life habitat of P. acnes; we tested our lipopeptide library in an attempt to create potent P. acnes-specific antimicrobial agents. Experimental Approach: Antimicrobial activity was determined by the minimal inhibitory concentration (MIC). Lipids from P. acnes were used to explore the mode of action. RAW264.7 cells respectively stimulated with LPS and P. acnes were used to measure the anti-inflammatory activity. Mice ears injected with P. acnes were used to assess the antimicrobial and anti-inflammatory effects of the peptides tested in vivo. Key Results: The most potent candidate, C16-KWKW, was observed to be more active against P. acnes, with an MIC of 2 μg•ml −1 , than against other non-targeted bacterial strains, such as Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. The mode of action of C16-KWKW was observed to be through interference with the integrity of bacterial membrane, thereby impairing membrane permeability and causing leakage of the inner contents of bacterial cells. In addition, C16-KWKW inhibited the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, and inducible NOS, stimulated by both LPS and P. acnes, thus showing potential anti-inflammatory activity, which was further assessed in animal studies in vivo. Conclusions and Implications: C16-KWKW is a lipopeptide displaying both anti-P. acnes and anti-inflammatory effects in vitro and in vivo, and exhibits potential as a treatment for acne vulgaris induced by P. acnes. 1 | INTRODUCTION Acne vulgaris is a chronic skin disorder that often develops on the face and upper trunk and is characterized by the formation of comedones, papules, nodules, pustules, and cysts (Aslam, Fleischer, & Feldman, 2015). In addition, the disease is always associated with an emotional and psychological impact, which can result in anxiety, reduced selfesteem, and depression or even thoughts of suicide (Tasoula et al., 2012). It affected more than 630 million people globally in 2015, thus being ranked as the eighth most common disease in the world (Disease, Injury, & Prevalence, 2016). Current research shows that several factors are involved in the development of this condition including an overgrowth of the skin commensal bacteria, obstruction of the follicle, excess production of sebum, and inflammation of the

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“…As widely described, the intrinsic fluorescence emission of tryptophan can be used to probe the changes that occur in the tertiary conformation of oligomeric species formed during the peptide and protein aggregation process. − Hence, we compared the fluorescence properties of the tryptophan residues in the PAs with those of the parent peptide in water. As shown in Figure , the emission spectrum of peptide E1P47 in water showed a maximum centered at 356 nm indicating the hydrophilic environment of the W residues.…”

Section: Resultsmentioning

confidence: 99%

Peptide Amphiphilic-Based Supramolecular Structures with Anti-HIV-1 Activity

et al. 2021

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In a previous work, we defined a novel HIV-1 fusion inhibitor peptide (E1P47) with a broad spectrum of activity against viruses from different clades, subtypes, and tropisms. With the aim to enhance its efficacy, in the present work we address the design and synthesis of several peptide amphiphiles (PAs) based on the E1P47 peptide sequence to target the lipid rafts of the cell membrane where the cell–cell fusion process takes place. We report the synthesis of novel PAs having a hydrophobic moiety covalently attached to the peptide sequence through a hydrophilic spacer of polyethylene glycol. Characterization of self-assembly in condensed phase and aqueous solution as well as their interaction with model membranes was analyzed by several biophysical methods. Our results demonstrated that the length of the spacer of polyethylene glycol, the position of the peptide conjugation as well as the type of the hydrophobic residue determine the antiviral activity of the construct. Peptide amphiphiles with one alkyl tail either in C-terminus ( C -PA monoalkyl ) or in N-terminus ( N -PA monoalkyl ) showed the highest anti-HIV-1 activities in the cellular model of TZM-bl cells or in a preclinical model of the human mucosal tissue explants.

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A facile and dynamic assay for the detection of peptide aggregation

Cited by 3 publications

References 21 publications

Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with a dual antibacterial and anti‐inflammatory action

Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with a dual antibacterial and anti‐inflammatory action

Retracted: Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with dual antibacterial and anti‐inflammatory action

Peptide Amphiphilic-Based Supramolecular Structures with Anti-HIV-1 Activity

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