A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels

Duan, Nuo; Wu, Shijia; Yu, Ye; Ma, Xiaoyuan; Xia, Yu; Chen, Xiujuan; Huang, Yukun; Wang, Zhouping

doi:10.1016/j.aca.2013.09.047

Cited by 74 publications

(32 citation statements)

References 32 publications

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“…The further research indicated that the detection limit of the electrochemical immunoassay was 13 cfu/mL for E. coli O157:H7 (3 σ ). This value is markedly smaller than 5000 [20], 3270 cfu/mL [2], 1200 [21], 150 cfu/mL [9], 83.7 cfu/mL [22], 30 cfu/mL [4], and 22 cfu/mL [8], and higher than 7 cfu/mL [23], 3 cfu/mL [24], and 2 cfu/mL [25]. The detection limit of 4.5 fg/ μ L [26] and 800 cells/mL [27] was also realized.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels

Chen

Yin

Lou³

2014

Journal of Analytical Methods in Chemistry

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Silica coated silver (Ag@SiO2) nanoparticles were prepared and characterized by transmission electron microscope (TEM) and UV-vis absorption, and the nanoparticles were used as labels in sandwich-type immunosensor of Escherichia coli O157:H7 (E. coli O157:H7). The labels involved in immunoreaction were dissolved by mixed acid of hydrofluoric acid and nitric acid, and the released Ag+ ions were electrochemical stripping analyzed (via differential pulse voltammetry, DPV) at poly(acrylic acid)/poly(diallyldimethylammonium chloride)/carbon nanotubes (PAA/PDCNT) modified glass carbon electrode (GCE), which obviously enhanced the signal of Ag+ stripping. Then, the number of E. coli O157:H7 could be indirectly reflected by the signal intensity of labeled Ag+. And the results showed that the DPV signals were proportional to the logarithm of the E. coli O157:H7 concentration in the range from 20 cfu/mL to 8.0 × 103 cfu/mL with the detection limit of 13 cfu/mL.

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Section: Resultsmentioning

confidence: 99%

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels

Chen

Yin

Lou³

2014

Journal of Analytical Methods in Chemistry

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“…Listeria , E. coli , Vibrio , E. sakazakii , and Streptococcus ).The same researchers used aptamer-conjugated, dual-color quantum dots to simultaneously detect Vibrio parahaemolyticus and Salmonella typhimurium . 23 Fluorescence-based nanoprobes containing aptamers show good promise for multiplex and sensitive detection of bacteria. However, their use in complex matrices may result in quenching of the fluorophores.…”

Section: Aptamer-based Detection Of Bacteriamentioning

confidence: 99%

Integrating recognition elements with nanomaterials for bacteria sensing

et al. 2017

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“…Few years back, we used aptasensors and dual fluorescence from green QDs and red QDs nanoparticles as donors and amorphous carbon nanotubes as acceptors could give the LOD of 25 and 35 cfu/mL in food sample for Vibrio parahaemolyticus and S. typhimurium, respectively (Duan et al 2013d). Interestingly, this method provided improved simultaneous and visual detection of pathogens based on QDs fluorescence which is linearly proportional to the target concentration.…”

Section: Salmonella Speciesmentioning

confidence: 99%

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

et al. 2017

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Foodborne ailments constitute a public health challenge and pose an incredible economic burden in healthcare system around the globe. This dilemma has urged authorities and other entities working in field of food quality control and supply chain to play a pivotal role in ensuring food safety. Analytical strategies have been developed using numerous systematic evolution of ligands by exponential enrichment (SELEX) methods to assure food safety. High-affinity and high-sensitivity ssDNA and RNA aptamers against pathogens have emerged as a novel strategy, as compared to the more resource-demanding and complicated biochemical test-based approaches. Thus, this review aims to focus on some methods used in the selection of specific bare, modified, and conjugated aptamers and on the further analysis of selected aptamers using flow cytometer or post-SELEX modifications for enhanced detection of frequently diagnosed foodborne bacteria such as Bacillus sp., Campylobacter jejuni, Escherichia sp., Salmonella sp., Staphylococcus aureus, Shigella sp., Listeria monocytogenes, and Streptococcus pyogenes and/or targeting their cell components towards attaining fast, sensitive, and selective methods for the detection of pathogens in food(s) or other sources.

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A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels

Cited by 74 publications

References 32 publications

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels

Integrating recognition elements with nanomaterials for bacteria sensing

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

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A dual-color flow cytometry protocol for the simultaneous detection of Vibrio parahaemolyticus and Salmonella typhimurium using aptamer conjugated quantum dots as labels

Cited by 74 publications

References 32 publications

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO2Nanoparticles as Labels

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO2Nanoparticles as Labels

Integrating recognition elements with nanomaterials for bacteria sensing

An Update on Aptamer-Based Multiplex System Approaches for the Detection of Common Foodborne Pathogens

Contact Info

Product

Resources

About

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels

Electrochemical Immunoassay ofEscherichia coliO157:H7 Using Ag@SiO₂Nanoparticles as Labels