A drivable optrode for use in chronic electrophysiology and optogenetic experiments

Stocke, Sanaya K.; Samuelsen, Chad L.

doi:10.1016/j.jneumeth.2020.108979

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…A drivable part which can lead the probe tip to break through the wrap and continue to function becomes a good choice. The drivable optoprobes are inspired by the micro-actuated structure of the electrodes [ 118 , 119 ], which can be customized according to the different optical components ( Figure 3 a,b) [ 97 , 116 ] or simpler 3D-printed molds [ 120 , 121 , 122 ]. The 3D-printed mold can also precisely control the adjustment step by twisting of the screw, shown in Figure 3 c, and the minimum step is as low as 320 μm [ 122 ].…”

Section: Optoprobesmentioning

confidence: 99%

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A Review: Research Progress of Neural Probes for Brain Research and Brain–Computer Interface

2022

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Neural probes, as an invasive physiological tool at the mesoscopic scale, can decipher the code of brain connections and communications from the cellular or even molecular level, and realize information fusion between the human body and external machines. In addition to traditional electrodes, two new types of neural probes have been developed in recent years: optoprobes based on optogenetics and magnetrodes that record neural magnetic signals. In this review, we give a comprehensive overview of these three kinds of neural probes. We firstly discuss the development of microelectrodes and strategies for their flexibility, which is mainly represented by the selection of flexible substrates and new electrode materials. Subsequently, the concept of optogenetics is introduced, followed by the review of several novel structures of optoprobes, which are divided into multifunctional optoprobes integrated with microfluidic channels, artifact-free optoprobes, three-dimensional drivable optoprobes, and flexible optoprobes. At last, we introduce the fundamental perspectives of magnetoresistive (MR) sensors and then review the research progress of magnetrodes based on it.

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Section: Optoprobesmentioning

confidence: 99%

“…( c ) The fully prepared drivable optrode with the 3D-printed acrylic microdrive and magnified view of the optrode tip. ( A ): Front view of the drivable optrode; ( B ): Magnified image of the optrode tip [ 122 ]. Reprinted from, copyright (2021), with permission from Elsevier.…”

Section: Figurementioning

confidence: 99%

A Review: Research Progress of Neural Probes for Brain Research and Brain–Computer Interface

2022

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“…Drivable electrodes were designed for chronic recording, which can puncture glial encapsulation to approach targeted neurons (Brosch et al 2021, Voroslakos et al 2021b. Larger rodents and non-human primates allow the application of relatively large electrodes, and as a result, most existing designs of drivable electrodes are too large and heavy for application in free-moving mice (Du Hoffmann et al 2011, Polo-Castillo et al 2019, Stocke and Samuelsen 2021. Using a mouse for biological research has many advantages, such as the ability to experimentally manipulate the mouse genome.…”

Section: Discussionmentioning

confidence: 99%

Low-cost and easy-fabrication lightweight drivable electrode array for multiple-regions electrophysiological recording in free-moving mice

et al. 2022

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Objective. Extracellular electrophysiology has been widely applied to neural circuit dissections. However, long-term multiregional recording in free-moving mice remains a challenge. Low-cost and easy-fabrication of elaborate drivable electrodes is required for their prevalence. Approach. A three-layer nested construct (OD ~1.80 mm, length ~10 mm, <0.1g) was recruited as a drivable component, which consisted of an ethylene-vinyl acetate copolymer (EVA) heat-shrinkable tube, non-closed loop ceramic bushing, and stainless ferrule with a bulge twining silver wire. The supporting and working components were equipped with drivable components to be assembled into a drivable microwire electrode array with a nested structure (drivable MEANS). Two drivable microwire electrode arrays were independently implanted for chronic recording in different brain areas at respective angles. An optic fiber was easily loaded into the drivable MEANS to achieve optogenetic modulation and electrophysiological recording simultaneously. Main results. The drivable MEANS had lightweight (~ 0.37 g), small (~ 15 mm ×15 mm × 4 mm), and low cost (≤ $64.62). Two drivable MEANS were simultaneously implanted in mice, and high-quality electrophysiological recordings could be applied ≥ 5 months after implantation in freely behaving animals. Electrophysiological recordings and analysis of the lateral septum (LS) and lateral hypothalamus (LH) in food-seeking behavior demonstrated that our drivable MEANS can be used to dissect the function of neural circuits. An optical fiber-integrated drivable MEANS (~ 0.47 g) was used to stimulate and record LS neurons, which suggested that changes in working components can achieve more functions than electrophysiological recordings, such as optical stimulation, drug release, and calcium imaging. Significance. Drivable MEANS is an easily fabricated, lightweight drivable microwire electrode array for multiple-region electrophysiological recording in free-moving mice. Our design is likely to be a valuable platform for both current and prospective users, as well as for developers of multifunctional electrodes for free-moving mice.

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Experimental Verification for Numerical Simulation of Thalamic Stimulation-Evoked Calcium-Sensitive Fluorescence and Electrophysiology with Self-Assembled Multifunctional Optrode

et al. 2023

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Owing to its capacity to eliminate a long-standing methodological limitation, fiber photometry can assist research gaining novel insight into neural systems. Fiber photometry can reveal artifact-free neural activity under deep brain stimulation (DBS). Although evoking neural potential with DBS is an effective method for mediating neural activity and neural function, the relationship between DBS-evoked neural Ca2+ change and DBS-evoked neural electrophysiology remains unknown. Therefore, in this study, a self-assembled optrode was demonstrated as a DBS stimulator and an optical biosensor capable of concurrently recording Ca2+ fluorescence and electrophysiological signals. Before the in vivo experiment, the volume of tissue activated (VTA) was estimated, and the simulated Ca2+ signals were presented using Monte Carlo (MC) simulation to approach the realistic in vivo environment. When VTA and the simulated Ca2+ signals were combined, the distribution of simulated Ca2+ fluorescence signals matched the VTA region. In addition, the in vivo experiment revealed a correlation between the local field potential (LFP) and the Ca2+ fluorescence signal in the evoked region, revealing the relationship between electrophysiology and the performance of neural Ca2+ concentration behavior. Concurrent with the VTA volume, simulated Ca2+ intensity, and the in vivo experiment, these data suggested that the behavior of neural electrophysiology was consistent with the phenomenon of Ca2+ influx to neurons.

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A drivable optrode for use in chronic electrophysiology and optogenetic experiments

Cited by 3 publications

References 25 publications

A Review: Research Progress of Neural Probes for Brain Research and Brain–Computer Interface

A Review: Research Progress of Neural Probes for Brain Research and Brain–Computer Interface

Low-cost and easy-fabrication lightweight drivable electrode array for multiple-regions electrophysiological recording in free-moving mice

Experimental Verification for Numerical Simulation of Thalamic Stimulation-Evoked Calcium-Sensitive Fluorescence and Electrophysiology with Self-Assembled Multifunctional Optrode

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