A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000

Almeida, Nalvo F.; Yan, Shuangchun; Lindeberg, Magdalen; Studholme, David J.; Schneider, David J.; Condon, Bradford; Liu, Haijie; Viana, Carlos Juliano M.; Warren, Andrew S.; Evans, Clive; Kemen, Eric; MacLean, Dan; Angot, Aurelie; Martin, Gregory B.; Jones, Jonathan D. G.; Collmer, Alan; Setúbal, João Carlos; Vinatzer, Boris A.

doi:10.1094/mpmi-22-1-0052

Cited by 126 publications

(107 citation statements)

References 54 publications

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“…Interestingly, DC3000 contains HopA1, and genome sequencing of P. syringae pv tomato strain T1 provides evidence for a hopA1 pseudogene (Almeida et al, 2009). This gene would encode a virtually identical HopA1 protein as in DC3000 except for a premature stop codon at amino acid position 55.…”

Section: Hopa1 Distribution In P Syringae Strainsmentioning

confidence: 99%

“…The P. syringae species is divided into a large number of distinct pathovars based on the plant host from which a strain was originally isolated. This and the ease of molecular manipulation of this facultative bacterial plant pathogen has made P. syringae an important experimental system in the study of plant innate immunity, the effector complement of plant pathogens, and the evolution and genomics of host range (Fouts et al, 2002;Rohmer et al, 2004;Chang et al, 2005;Almeida et al, 2009). In particular, P. syringae pv tomato strain DC3000 (DC3000) and P. syringae pv maculicola strain 4326 were found to be pathogenic on Arabidopsis (Dong et al, 1991;Whalen et al, 1991) and were used to characterize effector genes from other P. syringae pathovars and cognate Arabidopsis R genes.…”

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confidence: 99%

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Resistance to the Pseudomonas syringae Effector HopA1 Is Governed by the TIR-NBS-LRR Protein RPS6 and Is Enhanced by Mutations in SRFR1

et al. 2009

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The Pseudomonas syringae-Arabidopsis (Arabidopsis thaliana) interaction is an extensively studied plant-pathogen system. Arabidopsis possesses approximately 150 putative resistance genes encoding nucleotide binding site (NBS) and leucine-rich repeat (LRR) domain-containing proteins. The majority of these belong to the Toll/Interleukin-1 receptor (TIR)-NBS-LRR (TNL) class. Comparative studies with the coiled-coil-NBS-LRR genes RPS2, RPM1, and RPS5 and isogenic P. syringae strains expressing single corresponding avirulence genes have been particularly fruitful in dissecting specific and common resistance signaling components. However, the major TNL class is represented by a single known P. syringae resistance gene, RPS4. We previously identified hopA1 from P. syringae pv syringae strain 61 as an avirulence gene that signals through ENHANCED DISEASE SUSCEPTIBILITY1, indicating that the corresponding resistance gene RPS6 belongs to the TNL class. Here we report the identification of RPS6 based on a forward-genetic screen and map-based cloning. Among resistance proteins of known function, the deduced amino acid sequence of RPS6 shows highest similarity to the TNL resistance protein RAC1 that determines resistance to the oomycete pathogen Albugo candida. Similar to RPS4 and other TNL genes, RPS6 generates alternatively spliced transcripts, although the alternative transcript structures are RPS6 specific. We previously characterized SRFR1 as a negative regulator of avrRps4-triggered immunity. Interestingly, mutations in SRFR1 also enhanced HopA1-triggered immunity in rps6 mutants. In conclusion, the cloning of RPS6 and comparisons with RPS4 will contribute to a closer dissection of the TNL resistance pathway in Arabidopsis.

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Section: Hopa1 Distribution In P Syringae Strainsmentioning

confidence: 99%

mentioning

confidence: 99%

Resistance to the Pseudomonas syringae Effector HopA1 Is Governed by the TIR-NBS-LRR Protein RPS6 and Is Enhanced by Mutations in SRFR1

et al. 2009

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“…tomato DC3000 [4], P. syringae pv. tomato T1 [1], P. syringae pv. phaseolicola 1448A [21], P. syringae pv.…”

Section: Vol 191 2009 Achromobactin Biosynthesis By Pseudomonas Syrmentioning

confidence: 99%

Analysis of Achromobactin Biosynthesis byPseudomonas syringaepv. syringae B728a

Berti

Thomas

2009

J Bacteriol

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Pseudomonas syringae pv. syringae B728a is known to produce the siderophore pyoverdine under iron-limited conditions. It has also been proposed that this pathovar has the ability to produce a second siderophore, achromobactin. Here we present genetic and biochemical evidence supporting the hypothesis that P. syringae pv. syringae B728a produces both of these siderophores. We show that strains unable to synthesize either pyoverdine or achromobactin are unable to grow under iron-limiting conditions, which is consistent with these two molecules being the only siderophores synthesized by P. syringae pv. syringae B728a. Enzymes associated with achromobactin biosynthesis were purified and analyzed for substrate recognition. We showed that AcsD, AcsA, and AcsC together are able to condense citrate, ethanolamine, 2,4-diaminobutyrate, and ␣-ketoglutarate into achromobactin. Replacement of ethanolamine with ethylene diamine or 1,3-diaminopropane in these reactions resulted in the formation of achromobactin analogs that were biologically active. This work provides insights into the biosynthetic steps in the formation of achromobactin and is the first in vitro reconstitution of achromobactin biosynthesis.

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“…As more bacterial species are sequenced, a draft sequence of genomes closely related to a finished reference genome will serve as a valuable resource for comparative analysis. Draft genome sequences have been used for rapid development of diagnostic tools, comparison of gene repertoires, and identification of putative virulence factors (2,4,11).…”

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confidence: 99%

Genomic Analysis of Xanthomonas oryzae Isolates from Rice Grown in the United States Reveals Substantial Divergence from Known X. oryzae Pathovars

Triplett

Hamilton

Buell

et al. 2011

Appl Environ Microbiol

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The species Xanthomonas oryzae is comprised of two designated pathovars, both of which cause economically significant diseases of rice in Asia and Africa. Although X. oryzae is not considered endemic in the United States, an X. oryzae-like bacterium was isolated from U.S. rice and southern cutgrass in the late 1980s. The U.S. strains were weakly pathogenic and genetically distinct from characterized X. oryzae pathovars. In the current study, a draft genome sequence from two U.S. Xanthomonas strains revealed that the U.S. strains form a novel clade within the X. oryzae species, distinct from all strains known to cause significant yield loss. Comparative genome analysis revealed several putative gene clusters specific to the U.S. strains and supported previous reports that the U.S. strains lack transcriptional activator-like (TAL) effectors. In addition to phylogenetic and comparative analyses, the genome sequence was used for designing robust U.S. strain-specific primers, demonstrating the usefulness of a draft genome sequence in the rapid development of diagnostic tools.The species Xanthomonas oryzae is comprised of pathovars oryzae and oryzicola, the causative agents of bacterial leaf blight (BLB) and bacterial leaf streak (BLS) on rice, respectively (27). Although the two pathovars are closely related, BLB is a vascular disease characterized by marginal leaf lesions, while BLS affects parenchyma cells and results in leaf streaking. Both pathovars can cause substantial losses to rice production (27). X. oryzae has been designated a USDA select agent in the United States, and movement is restricted by several international quarantines (25,29).The finished genomes of three Asian X. oryzae pv. oryzae strains and one X. oryzae pv. oryzicola strain are available, facilitating in-depth comparative genomic analyses (22,28,35) (GenBank accession no. AAQN01000001). Whole-genome alignment revealed that the sequenced X. oryzae pv. oryzae strains, MAFF 311018, KACC 10331, and PXO99A, are very closely related (24, 35). X. oryzae pv. oryzicola clusters with the X. oryzae group, forming a branch distinct from X. oryzae pv. oryzae strains (24, 35). All the X. oryzae genomes are characterized by large numbers of insertion sequence (IS) elements, the major contributors to sequence diversity within the species (28, 35), and by various numbers of secreted transcriptional activator-like (TAL) effectors required for full virulence (35,42). The African X. oryzae pv. oryzae strains are different from Asian strains and more closely related to Asian X. oryzae pv. oryzicola. A specific and intriguing feature of African X. oryzae pv. oryzae strains is that the genome contains a smaller number of TAL effector and IS elements than the Asian strains (8). Bacterial blight has also been observed in South America (12, 23); strains isolated in Colombia are closely related to Asian strains (15,17).Although X. oryzae is not historically considered indigenous to the United States (27), strains of a yellow bacterium causing mild BLB-like symptoms we...

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A Draft Genome Sequence of Pseudomonas syringae pv. tomato T1 Reveals a Type III Effector Repertoire Significantly Divergent from That of Pseudomonas syringae pv. tomato DC3000

Cited by 126 publications

References 54 publications

Resistance to the Pseudomonas syringae Effector HopA1 Is Governed by the TIR-NBS-LRR Protein RPS6 and Is Enhanced by Mutations in SRFR1

Resistance to the Pseudomonas syringae Effector HopA1 Is Governed by the TIR-NBS-LRR Protein RPS6 and Is Enhanced by Mutations in SRFR1

Analysis of Achromobactin Biosynthesis byPseudomonas syringaepv. syringae B728a

Genomic Analysis of Xanthomonas oryzae Isolates from Rice Grown in the United States Reveals Substantial Divergence from Known X. oryzae Pathovars

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