A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction

Couto, Manuela Soares; Cordeiro, Rossana de Aguiar; Rocha, Marcos Fábio Gadelha; Grangeiro, Thalles B.; Junior, Natanael Pinheiro Leitão; Bandeira, Tereza de Jesus Pinheiro Gomes; Sidrim, José Júlio Costa; Brilhante, Raimunda Sâmia Nogueira

doi:10.1016/j.diagmicrobio.2009.05.010

Cited by 7 publications

(3 citation statements)

References 15 publications

(18 reference statements)

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“…Melioidosis presents in many forms, including acute, subacute, and chronic infections. Detection of B. pseudomallei by PCR assay has been applied to clinical samples, with some gene targets offering high specificity but variable sensitivity (32)(33)(34)(35), as well as frequent false-negative results because of low pathogen levels in blood (36). Our study took a different approach to melioidosis diagnostics-that of looking at host gene expression changes rather than pathogen identification.…”

Section: Discussionmentioning

confidence: 99%

Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis

et al. 2020

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Melioidosis is caused by Burkholderia pseudomallei and is predominantly seen in tropical regions. The clinical signs and symptoms of the disease are nonspecific and often result in misdiagnosis, failure of treatment, and poor clinical outcome. Septicemia with septic shock is the most common cause of death, with mortality rates above 40%. Bacterial culture is the gold standard for diagnosis, but it has low sensitivity and takes days to produce definitive results. Early laboratory diagnosis can help guide physicians to provide treatment specific to B. pseudomallei. In our study, we adapted host gene expression signatures obtained from microarray data of B. pseudomallei-infected cases to develop a real-time PCR diagnostic test using two differentially expressed genes, AIM2 (absent in melanoma 2) and FAM26F (family with sequence similarity 26, member F). We tested blood from 33 patients with B. pseudomallei infections and 29 patients with other bacterial infections to validate the test and determine cutoff values for use in a cascading diagnostic algorithm. Differentiation of septicemic melioidosis from other sepsis cases had a sensitivity of 82%, specificity of 93%, and negative and positive predictive values (NPV and PPV) of 82% and 93%, respectively. Separation of cases likely to be melioidosis from those unlikely to be melioidosis in nonbacteremic situations showed a sensitivity of 40%, specificity of 54%, and NPV and PPV of 44% and 50%, respectively. We suggest that our AIM2 and FAM26F expression combination algorithm could be beneficial for early melioidosis diagnosis, offering a result within 24 h of admission.

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Section: Discussionmentioning

confidence: 99%

Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis

et al. 2020

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“…Four siblings acquired acute infection after recreational exposure at the town dam and three of them died within a week [ 7 , 8 , 9 , 10 ]. By 2017, 30 cases had been diagnosed in Ceará [ 2 , 11 , 15 , 16 , 17 , 18 , 23 ]. Characterisation of clinical and environmental B. pseudomallei from Ceará showed that considerable genetic diversity is present [ 30 ].…”

Section: Review Of Published Melioidosis Cases and The Presence Ofmentioning

confidence: 99%

Melioidosis in South America

et al. 2018

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Melioidosis is an emerging disease in the Americas. This paper reviews confirmed cases, the presence of Burkholderia pseudomallei and the organization of national surveillance policies for melioidosis in South America. Confirmed cases in humans have been reported from Ecuador, Venezuela, Colombia, Brazil, and Peru. The bacterium has been isolated from the environment in Brazil and Peru. The state of Ceará, northeastern region of Brazil, is the only place where specific public strategies and policies for melioidosis have been developed. We also discuss the urgent need for health authorities in South America to pay greater attention to this disease, which has the potential to have a high impact on public health, and the importance of developing coordinated strategies amongst countries in this region.

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“…The remaining three follow-up studies used the non-nested procedure to detect Bp/Bm, and reported accuracies of 100% (Couto et al, 2009;Brilhante et al, 2012;Nandagopal et al, 2012). Two non-nested follow-up studies tested the 16-23s rRNA ITS assays for their ability to detect Bp/Bm in clinical samples, and reported clinical accuracies approaching 100% (Couto et al, 2009;Nandagopal et al, 2012).…”

Section: B Pseudomallei and B Malleimentioning

confidence: 99%

PCR-based Methodologies Used to Detect and Differentiate theBurkholderia pseudomalleicomplex:B. pseudomallei,B. mallei, andB. thailandensis

Lowe

March²,

Bunnell³

et al. 2014

Current Issues in Molecular Biology

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Methods for the rapid detection and differentiation of the Burkholderia pseudomallei complex comprising B. pseudomallei, B. mallei, and B. thailandensis, have been the topic of recent research due to the high degree of phenotypic and genotypic similarities of these species. B. pseudomallei and B. mallei are recognized by the CDC as tier 1 select agents. The high mortality rates of glanders and melioidosis, their potential use as bioweapons, and their low infectious dose, necessitate the need for rapid and accurate detection methods. Although B. thailandensis is generally avirulent in mammals, this species displays very similar phenotypic characteristics to that of B. pseudomallei. Optimal identification of these species remains problematic, due to the difficulty in developing a sensitive, selective, and accurate assay. The development of PCR technologies has revolutionized diagnostic testing and these detection methods have become popular due to their speed, sensitivity, and accuracy. The purpose of this review is to provide a comprehensive overview and evaluation of the advancements in PCR-based detection and differentiation methodologies for the B. pseudomallei complex, and examine their potential uses in diagnostic and environmental testing.

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A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction

Cited by 7 publications

References 15 publications

Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis

Adapting Microarray Gene Expression Signatures for Early Melioidosis Diagnosis

Melioidosis in South America

PCR-based Methodologies Used to Detect and Differentiate theBurkholderia pseudomalleicomplex:B. pseudomallei,B. mallei, andB. thailandensis

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