A CZE-UV Method for Saturated and Unsaturated Fatty Acids Determination in Hops

Duarte, Lucas Mattos; Amorim, Tatiane Lima; Adriano, Luiz Henrique Cantarino; Oliveira, Marcone Augusto Leal de

doi:10.1080/03610470.2019.1678377

Cited by 5 publications

(16 citation statements)

References 35 publications

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“…In 2020, on the other hand, Duarte et al evaluated full sample preparation for determining the total of C16:0, C18:0, C18:1c, and C18:2cc in hops [9]. For this, four conditions were assessed, three of which carried out an extraction followed by a saponification reaction in samples of sprayed hops, and one procedure carried out the direct saponification of the sprayed hops [9]. In procedure (A), the lipid extraction was adapted from the method by Hara and Radin, in which extracting solution composed of hexane/isopropanol 3:2 (v/v) and 0.5 M Na 2 SO 4 solution was added to the pulverized hops.…”

Section: Indirect Uv Detectionmentioning

confidence: 99%

“…In procedure (A), the lipid extraction was adapted from the method by Hara and Radin, in which extracting solution composed of hexane/isopropanol 3:2 (v/v) and 0.5 M Na 2 SO 4 solution was added to the pulverized hops. The organic extract had the solvent evaporated in a rotary evaporator at 40 • C [9]. Procedure (B) consisted of the Folch and Stanley method, in which a 2:1 (v/v) chloroform/MeOH extractor solution was added to the pulverized hops, followed by adding 0.9% (m/v) NaCl.…”

Section: Indirect Uv Detectionmentioning

confidence: 99%

“…CE equipment and analyzed under optimized conditions, as mentioned above. Procedure (D) involved only this saponification step in the sprayed hop samples [9].…”

Section: Indirect Uv Detectionmentioning

confidence: 99%

“…Since the 1990s, Capillary electrophoresis (CE) has been standing out in the scientific community as an up-and-coming alternative technique for determining the composition of FAs in food and biological matrices [2,8]. Compared to the other separation techniques, CE offers advantages such as shorter analysis time, the absence of the sample derivatization step, lower solvent consumption, nondedicated columns, and high analytical throughput [6,9,10].…”

Section: Introductionmentioning

confidence: 99%

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Fatty acids analysis by capillary electrophoresis: Fundamentals, advantages and applications

Geraldo,

Velasco,

Berlande

et al. 2023

Electrophoresis

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This review covers the know‐how of the Grupo de Química Analítica e Quimiometria regarding the analysis of fatty acids by capillary electrophoresis acquired over its 20 years of existence. Therefore, the fundamentals, advantages, and applications of this technique for analyzing different fatty acids in samples such as food, oils, cosmetics, and biological matrices are presented and discussed. Capillary electrophoresis is, thus, shown as an interesting and valuable separation technique for the target analysis of these analytes as an alternative to the gas chromatography coupled to flame ionization detection, as it offers advantages over the latter such as low analysis times, low sample and reagent consumption, the use of a nondedicated column, and simpler sample preparation. In addition, the methods shown in this literature review can be useful for quality control, adulteration, and health‐related research by regulatory agencies.

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Section: Indirect Uv Detectionmentioning

confidence: 99%

Section: Indirect Uv Detectionmentioning

confidence: 99%

“…CE equipment and analyzed under optimized conditions, as mentioned above. Procedure (D) involved only this saponification step in the sprayed hop samples [9].…”

Section: Indirect Uv Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fatty acids analysis by capillary electrophoresis: Fundamentals, advantages and applications

Geraldo,

Velasco,

Berlande

et al. 2023

Electrophoresis

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“…There are various ways to analyse the hops volatile aroma compounds. The important features of each sample preparation technique has been summarized in Table 3, apart from that, the hydrodistillation (Pistelli et al, 2018), gas chromatography with quadrupole accurate mass time‐of‐flight mass spectrometry (Yan et al, 2018), gas chromatography with accurate mass time‐of‐flight mass spectrometry (Yan et al, 2019), capillary zone electrophoresis with direct absorption ultraviolet detection (Duarte et al, 2020), simultaneous headspace gas chromatography‐mass spectrometry ion mobility spectrometry (Brendel et al, 2020), ultra‐microscale‐fractionation technique coupling ultra‐performance liquid chromatography mass spectrometry (McCallum et al, 2020) and pulsed electric field (Ntourtoglou et al, 2020) have been applied in hops aroma analyses.…”

Section: Chemical Constituentsmentioning

confidence: 99%

Chemical constituents and bioactivities of hops (Humulus lupulus L.) and their effects on beer‐related microorganisms

Sun

Wang

Yuan

et al. 2022

Food and Energy Security

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The female inflorescence of hops is traditionally used in the brewing industry to impart bitterness, aroma, flavour and longer shelf life to beer, where the bittering acids are the main compounds that enhance the quality of the beer. In recent years, a wide variety of bioactive compounds have been reported to be present in female flowers. Due to the growing consumer interests in natural ingredients, a great deal of research has been carried out in the last few years to find new sources of functional biomolecules. This paper reviews the recent applications of hops in the food, pharmaceutical and cosmetic industries and provides an overview of the bioactive potential of hops. In addition, the analytical techniques of hops compound are reviewed. The biological activities of hops compound such as antibacterial, antifungal, cardioprotective, antioxidant, anti‐inflammatory and anticancer are reviewed and the recent effects of hop bittering derivatives on beer‐related microbial activity are also presented. It is thus clear that hops have a high potential as bioactive components for use as preservatives in fresh foods to extend shelf life and as cosmetic formulations for skin care products as well as nutraceutical and health implications.

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Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

et al. 2020

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Fatty acids determination is of paramount importance for quality control and suitable labeling of edible oils, required by regulatory agencies in several countries, and fast methods for this determination are worldly desired. This review article aimed to explore the available analytical methods for vegetable and marine oils analyses employing CE, which can be a straightforward and faster alternative than GC methods for fatty acid determination, considering some purposes. CE usually offers the possibility of a rapid analysis with a simple preparation of the sample, without requiring specific columns, which are inherent advantages of the technique. Instrumental conditions and the key points about fatty acids determination employing the technique are highlighted, and the main challenges and perspectives are also approached. Potential use of CE for edible oil analyses has been demonstrated for research and routine, which can be of interest for industries, regulatory agencies, and edible oil researchers. Therefore, we have explored the analytical approaches described in the last decades, intending to spread the interest of CE methods for fatty acid monitoring, label accuracy assessment, and food authenticity evaluation of edible oils.

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A CZE-UV Method for Saturated and Unsaturated Fatty Acids Determination in Hops

Cited by 5 publications

References 35 publications

Fatty acids analysis by capillary electrophoresis: Fundamentals, advantages and applications

Fatty acids analysis by capillary electrophoresis: Fundamentals, advantages and applications

Chemical constituents and bioactivities of hops (Humulus lupulus L.) and their effects on beer‐related microorganisms

Capillary electromigration methods for fatty acids determination in vegetable and marine oils: A review

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